A galectin-specific signature in the gut delineates Crohn's disease and ulcerative colitis from other human inflammatory intestinal disorders
Inflammatory bowel diseases (IBD) are chronic and relapsing inflammatory conditions of the gastrointestinal tract including Crohn's disease (CD) and ulcerative colitis (UC). Galectins, defined by shared consensus amino acid sequence and affinity for β-galactosides, are critical modulators of th...
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paper:paper_09516433_v42_n1_p93_PapaGobbi2023-06-08T15:54:59Z A galectin-specific signature in the gut delineates Crohn's disease and ulcerative colitis from other human inflammatory intestinal disorders Toscano, Marta Alicia Biomarkers Crohn disease Galectins Inflammatory bowel diseases Ulcerative colitis ecalectin galectin galectin 3 galectin 4 messenger RNA 1-nitrohydroxyphenyl-N-benzoylalanine benzamide derivative galectin galectin 3 galectin 4 LGALS9 protein, human messenger RNA tyrosine adult aged Article colon biopsy controlled study Crohn disease discriminant analysis enteropathy female human human tissue inflammation intestine major clinical study male priority journal protein expression quantitative analysis remission reverse transcription polymerase chain reaction ulcerative colitis analogs and derivatives biopsy biosynthesis Crohn disease gastrointestinal tract gene expression regulation genetics intestine intestine mucosa metabolism pathology ulcerative colitis Adult Benzamides Biopsy Colitis, Ulcerative Crohn Disease Female Galectin 3 Galectin 4 Galectins Gastrointestinal Tract Gene Expression Regulation Humans Inflammation Intestinal Mucosa Intestines Male RNA, Messenger Tyrosine Inflammatory bowel diseases (IBD) are chronic and relapsing inflammatory conditions of the gastrointestinal tract including Crohn's disease (CD) and ulcerative colitis (UC). Galectins, defined by shared consensus amino acid sequence and affinity for β-galactosides, are critical modulators of the inflammatory response. However, the relevance of the galectin network in the pathogenesis of human IBD has not yet been explored. Here, we analyzed the expression of relevant members of the galectin family in intestinal biopsies, and identified their contribution as novel mucosal markers in IBD. Colonic biopsies were obtained from 59 IBD patients (22 CD and 37 UC), 9 patients with gut rejection after transplantation, 8 adult celiac patients, and 32 non-IBD donors. Galectin mRNA expression was analyzed by RT-PCR and qPCR using specific primers for individual galectins. A linear discriminant analysis (LDA) was used to analyze galectin expression in individual intestinal samples. Expression of common mucosal-associated galectins (Gal-1, -3, -4, -9) is dysregulated in inflamed tissues of IBD patients compared with non-inflamed IBD or control samples. LDA discriminated between different inflammation grades in active IBD and showed that remission IBD samples were clusterized with control samples. Galectin profiling could not distinguish CD and UC. Furthermore, inflamed IBD was discriminated from inflamed tissue of rejected gut in transplanted patients and duodenum of celiac patients, which could not be distinguished from control duodenum samples. The integrative analysis of galectins discriminated IBD from other intestinal inflammatory conditions and could be used as potential mucosal biomarker. © 2016 International Union of Biochemistry and Molecular Biology. Fil:Toscano, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09516433_v42_n1_p93_PapaGobbi http://hdl.handle.net/20.500.12110/paper_09516433_v42_n1_p93_PapaGobbi |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Biomarkers Crohn disease Galectins Inflammatory bowel diseases Ulcerative colitis ecalectin galectin galectin 3 galectin 4 messenger RNA 1-nitrohydroxyphenyl-N-benzoylalanine benzamide derivative galectin galectin 3 galectin 4 LGALS9 protein, human messenger RNA tyrosine adult aged Article colon biopsy controlled study Crohn disease discriminant analysis enteropathy female human human tissue inflammation intestine major clinical study male priority journal protein expression quantitative analysis remission reverse transcription polymerase chain reaction ulcerative colitis analogs and derivatives biopsy biosynthesis Crohn disease gastrointestinal tract gene expression regulation genetics intestine intestine mucosa metabolism pathology ulcerative colitis Adult Benzamides Biopsy Colitis, Ulcerative Crohn Disease Female Galectin 3 Galectin 4 Galectins Gastrointestinal Tract Gene Expression Regulation Humans Inflammation Intestinal Mucosa Intestines Male RNA, Messenger Tyrosine |
| spellingShingle |
Biomarkers Crohn disease Galectins Inflammatory bowel diseases Ulcerative colitis ecalectin galectin galectin 3 galectin 4 messenger RNA 1-nitrohydroxyphenyl-N-benzoylalanine benzamide derivative galectin galectin 3 galectin 4 LGALS9 protein, human messenger RNA tyrosine adult aged Article colon biopsy controlled study Crohn disease discriminant analysis enteropathy female human human tissue inflammation intestine major clinical study male priority journal protein expression quantitative analysis remission reverse transcription polymerase chain reaction ulcerative colitis analogs and derivatives biopsy biosynthesis Crohn disease gastrointestinal tract gene expression regulation genetics intestine intestine mucosa metabolism pathology ulcerative colitis Adult Benzamides Biopsy Colitis, Ulcerative Crohn Disease Female Galectin 3 Galectin 4 Galectins Gastrointestinal Tract Gene Expression Regulation Humans Inflammation Intestinal Mucosa Intestines Male RNA, Messenger Tyrosine Toscano, Marta Alicia A galectin-specific signature in the gut delineates Crohn's disease and ulcerative colitis from other human inflammatory intestinal disorders |
| topic_facet |
Biomarkers Crohn disease Galectins Inflammatory bowel diseases Ulcerative colitis ecalectin galectin galectin 3 galectin 4 messenger RNA 1-nitrohydroxyphenyl-N-benzoylalanine benzamide derivative galectin galectin 3 galectin 4 LGALS9 protein, human messenger RNA tyrosine adult aged Article colon biopsy controlled study Crohn disease discriminant analysis enteropathy female human human tissue inflammation intestine major clinical study male priority journal protein expression quantitative analysis remission reverse transcription polymerase chain reaction ulcerative colitis analogs and derivatives biopsy biosynthesis Crohn disease gastrointestinal tract gene expression regulation genetics intestine intestine mucosa metabolism pathology ulcerative colitis Adult Benzamides Biopsy Colitis, Ulcerative Crohn Disease Female Galectin 3 Galectin 4 Galectins Gastrointestinal Tract Gene Expression Regulation Humans Inflammation Intestinal Mucosa Intestines Male RNA, Messenger Tyrosine |
| description |
Inflammatory bowel diseases (IBD) are chronic and relapsing inflammatory conditions of the gastrointestinal tract including Crohn's disease (CD) and ulcerative colitis (UC). Galectins, defined by shared consensus amino acid sequence and affinity for β-galactosides, are critical modulators of the inflammatory response. However, the relevance of the galectin network in the pathogenesis of human IBD has not yet been explored. Here, we analyzed the expression of relevant members of the galectin family in intestinal biopsies, and identified their contribution as novel mucosal markers in IBD. Colonic biopsies were obtained from 59 IBD patients (22 CD and 37 UC), 9 patients with gut rejection after transplantation, 8 adult celiac patients, and 32 non-IBD donors. Galectin mRNA expression was analyzed by RT-PCR and qPCR using specific primers for individual galectins. A linear discriminant analysis (LDA) was used to analyze galectin expression in individual intestinal samples. Expression of common mucosal-associated galectins (Gal-1, -3, -4, -9) is dysregulated in inflamed tissues of IBD patients compared with non-inflamed IBD or control samples. LDA discriminated between different inflammation grades in active IBD and showed that remission IBD samples were clusterized with control samples. Galectin profiling could not distinguish CD and UC. Furthermore, inflamed IBD was discriminated from inflamed tissue of rejected gut in transplanted patients and duodenum of celiac patients, which could not be distinguished from control duodenum samples. The integrative analysis of galectins discriminated IBD from other intestinal inflammatory conditions and could be used as potential mucosal biomarker. © 2016 International Union of Biochemistry and Molecular Biology. |
| author |
Toscano, Marta Alicia |
| author_facet |
Toscano, Marta Alicia |
| author_sort |
Toscano, Marta Alicia |
| title |
A galectin-specific signature in the gut delineates Crohn's disease and ulcerative colitis from other human inflammatory intestinal disorders |
| title_short |
A galectin-specific signature in the gut delineates Crohn's disease and ulcerative colitis from other human inflammatory intestinal disorders |
| title_full |
A galectin-specific signature in the gut delineates Crohn's disease and ulcerative colitis from other human inflammatory intestinal disorders |
| title_fullStr |
A galectin-specific signature in the gut delineates Crohn's disease and ulcerative colitis from other human inflammatory intestinal disorders |
| title_full_unstemmed |
A galectin-specific signature in the gut delineates Crohn's disease and ulcerative colitis from other human inflammatory intestinal disorders |
| title_sort |
galectin-specific signature in the gut delineates crohn's disease and ulcerative colitis from other human inflammatory intestinal disorders |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09516433_v42_n1_p93_PapaGobbi http://hdl.handle.net/20.500.12110/paper_09516433_v42_n1_p93_PapaGobbi |
| work_keys_str_mv |
AT toscanomartaalicia agalectinspecificsignatureinthegutdelineatescrohnsdiseaseandulcerativecolitisfromotherhumaninflammatoryintestinaldisorders AT toscanomartaalicia galectinspecificsignatureinthegutdelineatescrohnsdiseaseandulcerativecolitisfromotherhumaninflammatoryintestinaldisorders |
| _version_ |
1768542514330992640 |