An antiviral principle present in a purified fraction from Melia azedarach L. leaf aqueous extract restrains herpes simplex virus type 1 propagation
Meliacine (MA), an antiviral principle isolated from leaves of Melia azedarach L., exhibits potent antiviral activity against herpes simplex virus type 1 (HSV-1) by inhibiting specific infected-cell polypeptides (ICPs) produced late in infection. Some of these are involved in DNA synthesis and in th...
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2002
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0951418X_v16_n4_p348_Alche http://hdl.handle.net/20.500.12110/paper_0951418X_v16_n4_p348_Alche |
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paper:paper_0951418X_v16_n4_p348_Alche2023-06-08T15:54:54Z An antiviral principle present in a purified fraction from Melia azedarach L. leaf aqueous extract restrains herpes simplex virus type 1 propagation Alche, Laura Edith Barquero, Andrea Alejandra Coto, Celia Esther Antiviral Herpes simplex virus type 1 Melia azedarach L. Meliacine antivirus agent beta galactosidase meliacine polypeptide unclassified drug virus DNA animal cell antiviral activity aqueous solution article controlled study cytoplasm cytoplasm vesicle DNA cleavage DNA packaging DNA synthesis drug mechanism drug synthesis enzyme activity Herpes simplex virus 1 medicinal plant melia azedarach molecular weight nonhuman plant leaf time Vero cell virus envelope virus inhibition virus nucleocapsid virus particle virus replication Animals Antiviral Agents Cell Transformation, Viral Cercopithecus aethiops DNA, Viral Herpesvirus 1, Human Meliaceae Plant Extracts Plant Leaves Vero Cells Viral Proteins Virus Replication Human herpesvirus 1 Melia azedarach Simplexvirus Meliacine (MA), an antiviral principle isolated from leaves of Melia azedarach L., exhibits potent antiviral activity against herpes simplex virus type 1 (HSV-1) by inhibiting specific infected-cell polypeptides (ICPs) produced late in infection. Some of these are involved in DNA synthesis and in the assembly of nucleocapsids. The present report provides additional evidence to elucidate the mode of action of MA against HSV-1. Time-of-addition experiments confirmed that MA affects a late event in the multiplication cycle of HSV-1. We showed that MA diminished the synthesis of viral DNA and inhibited the spread of infectious viral particles when HSV-1 that expresses β-galactosidase activity was used. In addition, the lack of a protein with an apparent MW of 55 KD was detected in MA-treated cell extracts. Ultrastructural analysis of infected cells showed that, in the case of MA treatment, a large number of unenveloped nucleocapsids accumulated in the cytoplasm and a minor proportion of mature virus was found in cytoplasmic vesicles. These findings suggest that MA exerts an antiviral action on both the synthesis of viral DNA and the maturation and egress of HSV-1 during the infection of Vero cells. Copyright © 2002 John Wiley & Sons, Ltd. Fil:Alché, L.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barquero, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coto, C.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2002 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0951418X_v16_n4_p348_Alche http://hdl.handle.net/20.500.12110/paper_0951418X_v16_n4_p348_Alche |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antiviral Herpes simplex virus type 1 Melia azedarach L. Meliacine antivirus agent beta galactosidase meliacine polypeptide unclassified drug virus DNA animal cell antiviral activity aqueous solution article controlled study cytoplasm cytoplasm vesicle DNA cleavage DNA packaging DNA synthesis drug mechanism drug synthesis enzyme activity Herpes simplex virus 1 medicinal plant melia azedarach molecular weight nonhuman plant leaf time Vero cell virus envelope virus inhibition virus nucleocapsid virus particle virus replication Animals Antiviral Agents Cell Transformation, Viral Cercopithecus aethiops DNA, Viral Herpesvirus 1, Human Meliaceae Plant Extracts Plant Leaves Vero Cells Viral Proteins Virus Replication Human herpesvirus 1 Melia azedarach Simplexvirus |
| spellingShingle |
Antiviral Herpes simplex virus type 1 Melia azedarach L. Meliacine antivirus agent beta galactosidase meliacine polypeptide unclassified drug virus DNA animal cell antiviral activity aqueous solution article controlled study cytoplasm cytoplasm vesicle DNA cleavage DNA packaging DNA synthesis drug mechanism drug synthesis enzyme activity Herpes simplex virus 1 medicinal plant melia azedarach molecular weight nonhuman plant leaf time Vero cell virus envelope virus inhibition virus nucleocapsid virus particle virus replication Animals Antiviral Agents Cell Transformation, Viral Cercopithecus aethiops DNA, Viral Herpesvirus 1, Human Meliaceae Plant Extracts Plant Leaves Vero Cells Viral Proteins Virus Replication Human herpesvirus 1 Melia azedarach Simplexvirus Alche, Laura Edith Barquero, Andrea Alejandra Coto, Celia Esther An antiviral principle present in a purified fraction from Melia azedarach L. leaf aqueous extract restrains herpes simplex virus type 1 propagation |
| topic_facet |
Antiviral Herpes simplex virus type 1 Melia azedarach L. Meliacine antivirus agent beta galactosidase meliacine polypeptide unclassified drug virus DNA animal cell antiviral activity aqueous solution article controlled study cytoplasm cytoplasm vesicle DNA cleavage DNA packaging DNA synthesis drug mechanism drug synthesis enzyme activity Herpes simplex virus 1 medicinal plant melia azedarach molecular weight nonhuman plant leaf time Vero cell virus envelope virus inhibition virus nucleocapsid virus particle virus replication Animals Antiviral Agents Cell Transformation, Viral Cercopithecus aethiops DNA, Viral Herpesvirus 1, Human Meliaceae Plant Extracts Plant Leaves Vero Cells Viral Proteins Virus Replication Human herpesvirus 1 Melia azedarach Simplexvirus |
| description |
Meliacine (MA), an antiviral principle isolated from leaves of Melia azedarach L., exhibits potent antiviral activity against herpes simplex virus type 1 (HSV-1) by inhibiting specific infected-cell polypeptides (ICPs) produced late in infection. Some of these are involved in DNA synthesis and in the assembly of nucleocapsids. The present report provides additional evidence to elucidate the mode of action of MA against HSV-1. Time-of-addition experiments confirmed that MA affects a late event in the multiplication cycle of HSV-1. We showed that MA diminished the synthesis of viral DNA and inhibited the spread of infectious viral particles when HSV-1 that expresses β-galactosidase activity was used. In addition, the lack of a protein with an apparent MW of 55 KD was detected in MA-treated cell extracts. Ultrastructural analysis of infected cells showed that, in the case of MA treatment, a large number of unenveloped nucleocapsids accumulated in the cytoplasm and a minor proportion of mature virus was found in cytoplasmic vesicles. These findings suggest that MA exerts an antiviral action on both the synthesis of viral DNA and the maturation and egress of HSV-1 during the infection of Vero cells. Copyright © 2002 John Wiley & Sons, Ltd. |
| author |
Alche, Laura Edith Barquero, Andrea Alejandra Coto, Celia Esther |
| author_facet |
Alche, Laura Edith Barquero, Andrea Alejandra Coto, Celia Esther |
| author_sort |
Alche, Laura Edith |
| title |
An antiviral principle present in a purified fraction from Melia azedarach L. leaf aqueous extract restrains herpes simplex virus type 1 propagation |
| title_short |
An antiviral principle present in a purified fraction from Melia azedarach L. leaf aqueous extract restrains herpes simplex virus type 1 propagation |
| title_full |
An antiviral principle present in a purified fraction from Melia azedarach L. leaf aqueous extract restrains herpes simplex virus type 1 propagation |
| title_fullStr |
An antiviral principle present in a purified fraction from Melia azedarach L. leaf aqueous extract restrains herpes simplex virus type 1 propagation |
| title_full_unstemmed |
An antiviral principle present in a purified fraction from Melia azedarach L. leaf aqueous extract restrains herpes simplex virus type 1 propagation |
| title_sort |
antiviral principle present in a purified fraction from melia azedarach l. leaf aqueous extract restrains herpes simplex virus type 1 propagation |
| publishDate |
2002 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0951418X_v16_n4_p348_Alche http://hdl.handle.net/20.500.12110/paper_0951418X_v16_n4_p348_Alche |
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