Natural resistance of BALB/c mice to herpes simplex virus-type 1 intraperitoneal infection is abrogated by a plant extract with in vitro anti- herpes activity
Adult BALB/c mice, genetically resistant to HSV-1 i.p. infection, were inoculated intraperitoneally with 1000 PFU of HSV-1 and treated by the same route with four consecutive doses of Melia azedarach L. aqueous green leaves extract (MA) each containing 80 antiviral units against HSV-1 replication in...
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1998
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0951418X_v12_n4_p250_Claus http://hdl.handle.net/20.500.12110/paper_0951418X_v12_n4_p250_Claus |
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paper:paper_0951418X_v12_n4_p250_Claus2023-06-08T15:54:54Z Natural resistance of BALB/c mice to herpes simplex virus-type 1 intraperitoneal infection is abrogated by a plant extract with in vitro anti- herpes activity Herpes virus infection Immunomodulator Melia azedarach L. Mice Natural resistance Plant antiviral Viral encephalitis antivirus agent immunomodulating agent melia asedarach extract plant extract unclassified drug age animal cell animal experiment animal model animal tissue antiviral activity article controlled study female herpes simplex virus 1 male mouse nonhuman peritoneum exudate peritonitis plant leaf sex difference virus detection virus immunity virus replication virus resistance Adult BALB/c mice, genetically resistant to HSV-1 i.p. infection, were inoculated intraperitoneally with 1000 PFU of HSV-1 and treated by the same route with four consecutive doses of Melia azedarach L. aqueous green leaves extract (MA) each containing 80 antiviral units against HSV-1 replication in Vero cells. Mice were injected 1 day and 6 h before infection and 1 and 2 days after infection. Mortality among untreated 45 day old female and male mice was around 35% for both sexes, whereas MA treatment increased mortality to 95% in females and to 50% in males. Virus was isolated from brains of treated animals. Enhanced susceptibility to HSV-1 infection upon treatment with MA extract was also observed in male mice 25 days old, before hormonal maturation, indicating that the impairment of natural resistance to HSV-1 infection is sex-linked. Treatment with MA extracts did not interfere with established specific immune protection nor with the induction of immune response to HSV-1. Virus was recovered from peritoneal exudate cells only in MA-treated animals. The abrogation of natural resistance of inbred mice to i.p. HSV-1 infection by pretreatment and early administration of the plant extract indicates that the in vivo expression of immunomodulating activities on receptor cells interfered with its potent antiviral activity displayed in vitro against HSV-1 replication. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0951418X_v12_n4_p250_Claus http://hdl.handle.net/20.500.12110/paper_0951418X_v12_n4_p250_Claus |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Herpes virus infection Immunomodulator Melia azedarach L. Mice Natural resistance Plant antiviral Viral encephalitis antivirus agent immunomodulating agent melia asedarach extract plant extract unclassified drug age animal cell animal experiment animal model animal tissue antiviral activity article controlled study female herpes simplex virus 1 male mouse nonhuman peritoneum exudate peritonitis plant leaf sex difference virus detection virus immunity virus replication virus resistance |
| spellingShingle |
Herpes virus infection Immunomodulator Melia azedarach L. Mice Natural resistance Plant antiviral Viral encephalitis antivirus agent immunomodulating agent melia asedarach extract plant extract unclassified drug age animal cell animal experiment animal model animal tissue antiviral activity article controlled study female herpes simplex virus 1 male mouse nonhuman peritoneum exudate peritonitis plant leaf sex difference virus detection virus immunity virus replication virus resistance Natural resistance of BALB/c mice to herpes simplex virus-type 1 intraperitoneal infection is abrogated by a plant extract with in vitro anti- herpes activity |
| topic_facet |
Herpes virus infection Immunomodulator Melia azedarach L. Mice Natural resistance Plant antiviral Viral encephalitis antivirus agent immunomodulating agent melia asedarach extract plant extract unclassified drug age animal cell animal experiment animal model animal tissue antiviral activity article controlled study female herpes simplex virus 1 male mouse nonhuman peritoneum exudate peritonitis plant leaf sex difference virus detection virus immunity virus replication virus resistance |
| description |
Adult BALB/c mice, genetically resistant to HSV-1 i.p. infection, were inoculated intraperitoneally with 1000 PFU of HSV-1 and treated by the same route with four consecutive doses of Melia azedarach L. aqueous green leaves extract (MA) each containing 80 antiviral units against HSV-1 replication in Vero cells. Mice were injected 1 day and 6 h before infection and 1 and 2 days after infection. Mortality among untreated 45 day old female and male mice was around 35% for both sexes, whereas MA treatment increased mortality to 95% in females and to 50% in males. Virus was isolated from brains of treated animals. Enhanced susceptibility to HSV-1 infection upon treatment with MA extract was also observed in male mice 25 days old, before hormonal maturation, indicating that the impairment of natural resistance to HSV-1 infection is sex-linked. Treatment with MA extracts did not interfere with established specific immune protection nor with the induction of immune response to HSV-1. Virus was recovered from peritoneal exudate cells only in MA-treated animals. The abrogation of natural resistance of inbred mice to i.p. HSV-1 infection by pretreatment and early administration of the plant extract indicates that the in vivo expression of immunomodulating activities on receptor cells interfered with its potent antiviral activity displayed in vitro against HSV-1 replication. |
| title |
Natural resistance of BALB/c mice to herpes simplex virus-type 1 intraperitoneal infection is abrogated by a plant extract with in vitro anti- herpes activity |
| title_short |
Natural resistance of BALB/c mice to herpes simplex virus-type 1 intraperitoneal infection is abrogated by a plant extract with in vitro anti- herpes activity |
| title_full |
Natural resistance of BALB/c mice to herpes simplex virus-type 1 intraperitoneal infection is abrogated by a plant extract with in vitro anti- herpes activity |
| title_fullStr |
Natural resistance of BALB/c mice to herpes simplex virus-type 1 intraperitoneal infection is abrogated by a plant extract with in vitro anti- herpes activity |
| title_full_unstemmed |
Natural resistance of BALB/c mice to herpes simplex virus-type 1 intraperitoneal infection is abrogated by a plant extract with in vitro anti- herpes activity |
| title_sort |
natural resistance of balb/c mice to herpes simplex virus-type 1 intraperitoneal infection is abrogated by a plant extract with in vitro anti- herpes activity |
| publishDate |
1998 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0951418X_v12_n4_p250_Claus http://hdl.handle.net/20.500.12110/paper_0951418X_v12_n4_p250_Claus |
| _version_ |
1768544874362044416 |