Leptin upregulates aquaporin 9 expression in human placenta in vitro
Aquaporins are integral membrane proteins that have permeability functions in many tissues. Aquaporin 9 may transport not only water but also small molecules, such as glycerol, monocarboxylates, purines and pyrimidines. Aquaporin 9 is expressed in syncytiotrophoblast of human term placenta, and it m...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09513590_v34_n2_p175_VilarinoGarcia http://hdl.handle.net/20.500.12110/paper_09513590_v34_n2_p175_VilarinoGarcia |
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paper:paper_09513590_v34_n2_p175_VilarinoGarcia2023-06-08T15:54:53Z Leptin upregulates aquaporin 9 expression in human placenta in vitro AQP9 GDM Leptin pregnancy transporter Trophoblastic cells aquaporin 9 beta tubulin leptin messenger RNA AQP9 protein, human aquaporin leptin Article controlled study female gene expression human human cell human tissue in vitro study placenta priority journal protein expression protein function regulatory mechanism transcription regulation trophoblast upregulation adult cesarean section cytology gene expression regulation genetics glycosylation immunoblotting metabolism osmolarity placenta pregnancy protein processing reproducibility reverse transcription polymerase chain reaction term birth tissue culture technique upregulation Adult Aquaporins Cesarean Section Female Gene Expression Regulation, Developmental Glycosylation Humans Immunoblotting Leptin Osmolar Concentration Placenta Pregnancy Protein Processing, Post-Translational Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction Term Birth Tissue Culture Techniques Trophoblasts Up-Regulation Aquaporins are integral membrane proteins that have permeability functions in many tissues. Aquaporin 9 may transport not only water but also small molecules, such as glycerol, monocarboxylates, purines and pyrimidines. Aquaporin 9 is expressed in syncytiotrophoblast of human term placenta, and it may contribute to the embryonic/fetal growth and survival. We have previously found that Aquaporin 9 expression levels seem to be increased in placenta from gestational diabetes. Since leptin plasma levels and leptin expression are increased in placenta from gestational diabetes, we aimed to study the possible role of leptin on Aquaporin 9 expression in human placenta in vitro. The present work shows that leptin produces a dose-dependent increase of Aquaporin 9 expression, resulting in an increase in Aquaporin-9 protein in human trophoblast explants. © 2017 Informa UK Limited, trading as Taylor & Francis Group. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09513590_v34_n2_p175_VilarinoGarcia http://hdl.handle.net/20.500.12110/paper_09513590_v34_n2_p175_VilarinoGarcia |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
AQP9 GDM Leptin pregnancy transporter Trophoblastic cells aquaporin 9 beta tubulin leptin messenger RNA AQP9 protein, human aquaporin leptin Article controlled study female gene expression human human cell human tissue in vitro study placenta priority journal protein expression protein function regulatory mechanism transcription regulation trophoblast upregulation adult cesarean section cytology gene expression regulation genetics glycosylation immunoblotting metabolism osmolarity placenta pregnancy protein processing reproducibility reverse transcription polymerase chain reaction term birth tissue culture technique upregulation Adult Aquaporins Cesarean Section Female Gene Expression Regulation, Developmental Glycosylation Humans Immunoblotting Leptin Osmolar Concentration Placenta Pregnancy Protein Processing, Post-Translational Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction Term Birth Tissue Culture Techniques Trophoblasts Up-Regulation |
| spellingShingle |
AQP9 GDM Leptin pregnancy transporter Trophoblastic cells aquaporin 9 beta tubulin leptin messenger RNA AQP9 protein, human aquaporin leptin Article controlled study female gene expression human human cell human tissue in vitro study placenta priority journal protein expression protein function regulatory mechanism transcription regulation trophoblast upregulation adult cesarean section cytology gene expression regulation genetics glycosylation immunoblotting metabolism osmolarity placenta pregnancy protein processing reproducibility reverse transcription polymerase chain reaction term birth tissue culture technique upregulation Adult Aquaporins Cesarean Section Female Gene Expression Regulation, Developmental Glycosylation Humans Immunoblotting Leptin Osmolar Concentration Placenta Pregnancy Protein Processing, Post-Translational Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction Term Birth Tissue Culture Techniques Trophoblasts Up-Regulation Leptin upregulates aquaporin 9 expression in human placenta in vitro |
| topic_facet |
AQP9 GDM Leptin pregnancy transporter Trophoblastic cells aquaporin 9 beta tubulin leptin messenger RNA AQP9 protein, human aquaporin leptin Article controlled study female gene expression human human cell human tissue in vitro study placenta priority journal protein expression protein function regulatory mechanism transcription regulation trophoblast upregulation adult cesarean section cytology gene expression regulation genetics glycosylation immunoblotting metabolism osmolarity placenta pregnancy protein processing reproducibility reverse transcription polymerase chain reaction term birth tissue culture technique upregulation Adult Aquaporins Cesarean Section Female Gene Expression Regulation, Developmental Glycosylation Humans Immunoblotting Leptin Osmolar Concentration Placenta Pregnancy Protein Processing, Post-Translational Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction Term Birth Tissue Culture Techniques Trophoblasts Up-Regulation |
| description |
Aquaporins are integral membrane proteins that have permeability functions in many tissues. Aquaporin 9 may transport not only water but also small molecules, such as glycerol, monocarboxylates, purines and pyrimidines. Aquaporin 9 is expressed in syncytiotrophoblast of human term placenta, and it may contribute to the embryonic/fetal growth and survival. We have previously found that Aquaporin 9 expression levels seem to be increased in placenta from gestational diabetes. Since leptin plasma levels and leptin expression are increased in placenta from gestational diabetes, we aimed to study the possible role of leptin on Aquaporin 9 expression in human placenta in vitro. The present work shows that leptin produces a dose-dependent increase of Aquaporin 9 expression, resulting in an increase in Aquaporin-9 protein in human trophoblast explants. © 2017 Informa UK Limited, trading as Taylor & Francis Group. |
| title |
Leptin upregulates aquaporin 9 expression in human placenta in vitro |
| title_short |
Leptin upregulates aquaporin 9 expression in human placenta in vitro |
| title_full |
Leptin upregulates aquaporin 9 expression in human placenta in vitro |
| title_fullStr |
Leptin upregulates aquaporin 9 expression in human placenta in vitro |
| title_full_unstemmed |
Leptin upregulates aquaporin 9 expression in human placenta in vitro |
| title_sort |
leptin upregulates aquaporin 9 expression in human placenta in vitro |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09513590_v34_n2_p175_VilarinoGarcia http://hdl.handle.net/20.500.12110/paper_09513590_v34_n2_p175_VilarinoGarcia |
| _version_ |
1768546077645996032 |