A rapid method for the screening of plasmids from Clostridium acetobutylicum and other Clostridium sp.

A rapid method was developed for plasmid DNA screening from micro volume cultures of Clostridium acetobutylicum. It involves protoplastization by lysozyme, nuclease inhibition by incorporating EDTA or DEP, followed by lysis with high concentration of SDS. The whole lysate is applied directly to elec...

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Autores principales: Schijman, Alejandro Gabriel, Floccari, Mirtha Elena, Méndez, Beatriz Silvia
Publicado: 1987
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0951208X_v1_n3_p163_Schijman
http://hdl.handle.net/20.500.12110/paper_0951208X_v1_n3_p163_Schijman
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling paper:paper_0951208X_v1_n3_p163_Schijman2023-06-08T15:54:52Z A rapid method for the screening of plasmids from Clostridium acetobutylicum and other Clostridium sp. Schijman, Alejandro Gabriel Floccari, Mirtha Elena Méndez, Beatriz Silvia A rapid method was developed for plasmid DNA screening from micro volume cultures of Clostridium acetobutylicum. It involves protoplastization by lysozyme, nuclease inhibition by incorporating EDTA or DEP, followed by lysis with high concentration of SDS. The whole lysate is applied directly to electrophoretic analysis. © 1987 Chapman & Hall. Fil:Schijman, A.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Floccari, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Méndez, B.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1987 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0951208X_v1_n3_p163_Schijman http://hdl.handle.net/20.500.12110/paper_0951208X_v1_n3_p163_Schijman
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
description A rapid method was developed for plasmid DNA screening from micro volume cultures of Clostridium acetobutylicum. It involves protoplastization by lysozyme, nuclease inhibition by incorporating EDTA or DEP, followed by lysis with high concentration of SDS. The whole lysate is applied directly to electrophoretic analysis. © 1987 Chapman & Hall.
author Schijman, Alejandro Gabriel
Floccari, Mirtha Elena
Méndez, Beatriz Silvia
spellingShingle Schijman, Alejandro Gabriel
Floccari, Mirtha Elena
Méndez, Beatriz Silvia
A rapid method for the screening of plasmids from Clostridium acetobutylicum and other Clostridium sp.
author_facet Schijman, Alejandro Gabriel
Floccari, Mirtha Elena
Méndez, Beatriz Silvia
author_sort Schijman, Alejandro Gabriel
title A rapid method for the screening of plasmids from Clostridium acetobutylicum and other Clostridium sp.
title_short A rapid method for the screening of plasmids from Clostridium acetobutylicum and other Clostridium sp.
title_full A rapid method for the screening of plasmids from Clostridium acetobutylicum and other Clostridium sp.
title_fullStr A rapid method for the screening of plasmids from Clostridium acetobutylicum and other Clostridium sp.
title_full_unstemmed A rapid method for the screening of plasmids from Clostridium acetobutylicum and other Clostridium sp.
title_sort rapid method for the screening of plasmids from clostridium acetobutylicum and other clostridium sp.
publishDate 1987
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0951208X_v1_n3_p163_Schijman
http://hdl.handle.net/20.500.12110/paper_0951208X_v1_n3_p163_Schijman
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