Cytolocalization of the PhoP response regulator in Salmonella enterica: Modulation by extracellular Mg2+ and by the SCV environment

The PhoP/PhoQ two-component system plays an essential role regulating numerous virulence phenotypes in Salmonella enterica. Previous work showed that PhoQ, the sensor protein, switches between the kinase- and the phosphatase-dominant state in response to environmental Mg2+ availability. This switch...

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Autores principales:	Spagnuolo, Carla Cecilia, Jares, Elizabeth Andrea
Publicado:	2008
Materias:	bacterial protein magnesium ion mutant protein PhoP protein protein phoq unclassified drug animal cell article cell vacuole cellular distribution controlled study cytoplasm fluorescence microscopy in vitro study macrophage nonhuman priority journal protein expression protein localization Salmonella enterica transcription initiation Animals Bacterial Proteins Cell Line Cytoplasm Macrophages Magnesium Mice Microscopy, Fluorescence Bacteria (microorganisms) Salmonella Strawberry crinkle virus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0950382X_v70_n2_p479_Sciara http://hdl.handle.net/20.500.12110/paper_0950382X_v70_n2_p479_Sciara
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_0950382X_v70_n2_p479_Sciara
record_format	dspace
spelling	paper:paper_0950382X_v70_n2_p479_Sciara2023-06-08T15:54:26Z Cytolocalization of the PhoP response regulator in Salmonella enterica: Modulation by extracellular Mg2+ and by the SCV environment Spagnuolo, Carla Cecilia Jares, Elizabeth Andrea bacterial protein magnesium ion mutant protein PhoP protein protein phoq unclassified drug animal cell article cell vacuole cellular distribution controlled study cytoplasm fluorescence microscopy in vitro study macrophage nonhuman priority journal protein expression protein localization Salmonella enterica transcription initiation Animals Bacterial Proteins Cell Line Cytoplasm Macrophages Magnesium Mice Microscopy, Fluorescence Salmonella enterica Bacteria (microorganisms) Salmonella Salmonella enterica Strawberry crinkle virus The PhoP/PhoQ two-component system plays an essential role regulating numerous virulence phenotypes in Salmonella enterica. Previous work showed that PhoQ, the sensor protein, switches between the kinase- and the phosphatase-dominant state in response to environmental Mg2+ availability. This switch defines the PhoP phosphorylation status and, as a result, the transcriptional activity of this regulator. In this work, using the FlAsH labelling technique, we examine PhoP cytolocalization in response to extracellular Mg2+ limitation in vitro and to the Salmonella-containing vacuole (SCV) environment in macrophage cells. We demonstrate that in these PhoP/PhoQ-inducing environments PhoP displays preferential localization to one cell pole, while being homogeneously distributed in the bacterial cytoplasm in repressing conditions. Polar localization is lost in the absence of PhoQ or when a non-phosphorylatable PhoPD52A mutant is expressed. However, when PhoP transcriptional activation is achieved in a Mg2+- and PhoQ-independent manner, PhoP regains asymmetric polar localization. In addition, we show that, in the analysed conditions, PhoQ cellular distribution does not parallel PhoP location pattern. These findings reveal that PhoP cellular location is dynamic and conditioned by its environmentally defined transcriptional status, showing a new insight in the PhoP/PhoQ system mechanism. © 2008 The Authors. Fil:Spagnuolo, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Jares-Erijman, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2008 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0950382X_v70_n2_p479_Sciara http://hdl.handle.net/20.500.12110/paper_0950382X_v70_n2_p479_Sciara
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	bacterial protein magnesium ion mutant protein PhoP protein protein phoq unclassified drug animal cell article cell vacuole cellular distribution controlled study cytoplasm fluorescence microscopy in vitro study macrophage nonhuman priority journal protein expression protein localization Salmonella enterica transcription initiation Animals Bacterial Proteins Cell Line Cytoplasm Macrophages Magnesium Mice Microscopy, Fluorescence Salmonella enterica Bacteria (microorganisms) Salmonella Salmonella enterica Strawberry crinkle virus
spellingShingle	bacterial protein magnesium ion mutant protein PhoP protein protein phoq unclassified drug animal cell article cell vacuole cellular distribution controlled study cytoplasm fluorescence microscopy in vitro study macrophage nonhuman priority journal protein expression protein localization Salmonella enterica transcription initiation Animals Bacterial Proteins Cell Line Cytoplasm Macrophages Magnesium Mice Microscopy, Fluorescence Salmonella enterica Bacteria (microorganisms) Salmonella Salmonella enterica Strawberry crinkle virus Spagnuolo, Carla Cecilia Jares, Elizabeth Andrea Cytolocalization of the PhoP response regulator in Salmonella enterica: Modulation by extracellular Mg2+ and by the SCV environment
topic_facet	bacterial protein magnesium ion mutant protein PhoP protein protein phoq unclassified drug animal cell article cell vacuole cellular distribution controlled study cytoplasm fluorescence microscopy in vitro study macrophage nonhuman priority journal protein expression protein localization Salmonella enterica transcription initiation Animals Bacterial Proteins Cell Line Cytoplasm Macrophages Magnesium Mice Microscopy, Fluorescence Salmonella enterica Bacteria (microorganisms) Salmonella Salmonella enterica Strawberry crinkle virus
description	The PhoP/PhoQ two-component system plays an essential role regulating numerous virulence phenotypes in Salmonella enterica. Previous work showed that PhoQ, the sensor protein, switches between the kinase- and the phosphatase-dominant state in response to environmental Mg2+ availability. This switch defines the PhoP phosphorylation status and, as a result, the transcriptional activity of this regulator. In this work, using the FlAsH labelling technique, we examine PhoP cytolocalization in response to extracellular Mg2+ limitation in vitro and to the Salmonella-containing vacuole (SCV) environment in macrophage cells. We demonstrate that in these PhoP/PhoQ-inducing environments PhoP displays preferential localization to one cell pole, while being homogeneously distributed in the bacterial cytoplasm in repressing conditions. Polar localization is lost in the absence of PhoQ or when a non-phosphorylatable PhoPD52A mutant is expressed. However, when PhoP transcriptional activation is achieved in a Mg2+- and PhoQ-independent manner, PhoP regains asymmetric polar localization. In addition, we show that, in the analysed conditions, PhoQ cellular distribution does not parallel PhoP location pattern. These findings reveal that PhoP cellular location is dynamic and conditioned by its environmentally defined transcriptional status, showing a new insight in the PhoP/PhoQ system mechanism. © 2008 The Authors.
author	Spagnuolo, Carla Cecilia Jares, Elizabeth Andrea
author_facet	Spagnuolo, Carla Cecilia Jares, Elizabeth Andrea
author_sort	Spagnuolo, Carla Cecilia
title	Cytolocalization of the PhoP response regulator in Salmonella enterica: Modulation by extracellular Mg2+ and by the SCV environment
title_short	Cytolocalization of the PhoP response regulator in Salmonella enterica: Modulation by extracellular Mg2+ and by the SCV environment
title_full	Cytolocalization of the PhoP response regulator in Salmonella enterica: Modulation by extracellular Mg2+ and by the SCV environment
title_fullStr	Cytolocalization of the PhoP response regulator in Salmonella enterica: Modulation by extracellular Mg2+ and by the SCV environment
title_full_unstemmed	Cytolocalization of the PhoP response regulator in Salmonella enterica: Modulation by extracellular Mg2+ and by the SCV environment
title_sort	cytolocalization of the phop response regulator in salmonella enterica: modulation by extracellular mg2+ and by the scv environment
publishDate	2008
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0950382X_v70_n2_p479_Sciara http://hdl.handle.net/20.500.12110/paper_0950382X_v70_n2_p479_Sciara
work_keys_str_mv	AT spagnuolocarlacecilia cytolocalizationofthephopresponseregulatorinsalmonellaentericamodulationbyextracellularmg2andbythescvenvironment AT jareselizabethandrea cytolocalizationofthephopresponseregulatorinsalmonellaentericamodulationbyextracellularmg2andbythescvenvironment
_version_	1768546593011662848

Cytolocalization of the PhoP response regulator in Salmonella enterica: Modulation by extracellular Mg2+ and by the SCV environment

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