DNA supercoiling and thermal regulation of unsaturated fatty acid synthesis in Bacillus subtilis
Bacillus subtilis growing at 37° C synthesizes, almost exclusively, saturated fatty acids. However, when a culture growing at 37°C is transferred to 20°C, the synthesis of unsaturated fatty acids is induced. The addition of the DNA gyrase inhibitor novobiocin specifically prevented the induction of...
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1994
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0950382X_v11_n5_p933_Grau http://hdl.handle.net/20.500.12110/paper_0950382X_v11_n5_p933_Grau |
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paper:paper_0950382X_v11_n5_p933_Grau2023-06-08T15:54:25Z DNA supercoiling and thermal regulation of unsaturated fatty acid synthesis in Bacillus subtilis Glikin, Gerardo Claudio novobiocin unsaturated fatty acid article bacillus subtilis dna supercoiling fatty acid synthesis nonhuman priority journal temperature Bacillus subtilis Bacterial Proteins DNA Topoisomerases, Type II DNA, Bacterial DNA, Superhelical Fatty Acids, Unsaturated Gene Expression Regulation, Bacterial Novobiocin Support, Non-U.S. Gov't Temperature Bacillus subtilis Bacillus subtilis growing at 37° C synthesizes, almost exclusively, saturated fatty acids. However, when a culture growing at 37°C is transferred to 20°C, the synthesis of unsaturated fatty acids is induced. The addition of the DNA gyrase inhibitor novobiocin specifically prevented the induction of unsaturated fatty acid synthesis at 20° C. Furthermore, it was determined that plasmid DNA isolated from cells growing at 20°C was significantly more negatively supercoiled than the equivalent DNA isolated from cells growing at 37°C. The overall results agree with the hypothesis that an increase in DNA supercoiling associated with a temperature downshift could regulate the unsaturated fatty acids synthesis in B. subtilis. Copyright © 1994, Wiley Blackwell. All rights reserved Fil:Glikin, G.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1994 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0950382X_v11_n5_p933_Grau http://hdl.handle.net/20.500.12110/paper_0950382X_v11_n5_p933_Grau |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
novobiocin unsaturated fatty acid article bacillus subtilis dna supercoiling fatty acid synthesis nonhuman priority journal temperature Bacillus subtilis Bacterial Proteins DNA Topoisomerases, Type II DNA, Bacterial DNA, Superhelical Fatty Acids, Unsaturated Gene Expression Regulation, Bacterial Novobiocin Support, Non-U.S. Gov't Temperature Bacillus subtilis |
| spellingShingle |
novobiocin unsaturated fatty acid article bacillus subtilis dna supercoiling fatty acid synthesis nonhuman priority journal temperature Bacillus subtilis Bacterial Proteins DNA Topoisomerases, Type II DNA, Bacterial DNA, Superhelical Fatty Acids, Unsaturated Gene Expression Regulation, Bacterial Novobiocin Support, Non-U.S. Gov't Temperature Bacillus subtilis Glikin, Gerardo Claudio DNA supercoiling and thermal regulation of unsaturated fatty acid synthesis in Bacillus subtilis |
| topic_facet |
novobiocin unsaturated fatty acid article bacillus subtilis dna supercoiling fatty acid synthesis nonhuman priority journal temperature Bacillus subtilis Bacterial Proteins DNA Topoisomerases, Type II DNA, Bacterial DNA, Superhelical Fatty Acids, Unsaturated Gene Expression Regulation, Bacterial Novobiocin Support, Non-U.S. Gov't Temperature Bacillus subtilis |
| description |
Bacillus subtilis growing at 37° C synthesizes, almost exclusively, saturated fatty acids. However, when a culture growing at 37°C is transferred to 20°C, the synthesis of unsaturated fatty acids is induced. The addition of the DNA gyrase inhibitor novobiocin specifically prevented the induction of unsaturated fatty acid synthesis at 20° C. Furthermore, it was determined that plasmid DNA isolated from cells growing at 20°C was significantly more negatively supercoiled than the equivalent DNA isolated from cells growing at 37°C. The overall results agree with the hypothesis that an increase in DNA supercoiling associated with a temperature downshift could regulate the unsaturated fatty acids synthesis in B. subtilis. Copyright © 1994, Wiley Blackwell. All rights reserved |
| author |
Glikin, Gerardo Claudio |
| author_facet |
Glikin, Gerardo Claudio |
| author_sort |
Glikin, Gerardo Claudio |
| title |
DNA supercoiling and thermal regulation of unsaturated fatty acid synthesis in Bacillus subtilis |
| title_short |
DNA supercoiling and thermal regulation of unsaturated fatty acid synthesis in Bacillus subtilis |
| title_full |
DNA supercoiling and thermal regulation of unsaturated fatty acid synthesis in Bacillus subtilis |
| title_fullStr |
DNA supercoiling and thermal regulation of unsaturated fatty acid synthesis in Bacillus subtilis |
| title_full_unstemmed |
DNA supercoiling and thermal regulation of unsaturated fatty acid synthesis in Bacillus subtilis |
| title_sort |
dna supercoiling and thermal regulation of unsaturated fatty acid synthesis in bacillus subtilis |
| publishDate |
1994 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0950382X_v11_n5_p933_Grau http://hdl.handle.net/20.500.12110/paper_0950382X_v11_n5_p933_Grau |
| work_keys_str_mv |
AT glikingerardoclaudio dnasupercoilingandthermalregulationofunsaturatedfattyacidsynthesisinbacillussubtilis |
| _version_ |
1768541570092498944 |