A Highly Sensitive Fluorogenic Probe for Imaging Glycoproteins and Mucine Activity in Live Cells in the Near-Infrared Region
A novel fluorescent molecular probe is reported, which is able to detect glycoproteins, especially mucins, with high sensitivity and with a turn-on response along with a large Stokes shift (>130 nm), within the biologically active window. The probe contains an aminotricarbocyanine as the fluoresc...
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2018
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09476539_v24_n24_p6344_SamaniegoLopez http://hdl.handle.net/20.500.12110/paper_09476539_v24_n24_p6344_SamaniegoLopez |
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paper:paper_09476539_v24_n24_p6344_SamaniegoLopez2023-06-08T15:54:00Z A Highly Sensitive Fluorogenic Probe for Imaging Glycoproteins and Mucine Activity in Live Cells in the Near-Infrared Region boronolectin cyanine dyes/pigments fluorescent probes glycoprotein Fluorescence Glycoproteins Infrared devices Boronolectin Cyanine Dyes/pigments Excellent solubility Fluorescent probes Fluorescent reporter Near infrared region Near-infrared emissions Probes boron derivative fluorescent dye glycoprotein mucin chemical structure chemistry drug effect epithelium cell human near infrared spectroscopy procedures Boron Compounds Epithelial Cells Fluorescent Dyes Glycoproteins Humans Molecular Structure Mucins Spectroscopy, Near-Infrared A novel fluorescent molecular probe is reported, which is able to detect glycoproteins, especially mucins, with high sensitivity and with a turn-on response along with a large Stokes shift (>130 nm), within the biologically active window. The probe contains an aminotricarbocyanine as the fluorescent reporter with a linked benzoboroxole as the recognition unit, which operates through a dynamic covalent reaction between the boronic hemiester residue of the receptor and cis-diols of the analyte. The superior selectivity of the probe is displayed by the labeling of mucins present in Calu-3 cells. The new benzoboroxole fluorescent derivative gathers together key properties to make it a highly rated molecular probe: specificity, excellent solubility in water, and off–on near infrared emission. This probe is expected to be an excellent tool for imaging intracellular mucin to evaluate mucus-related diseases as well as a sensing strategy towards glycosylated structures with a high potential for theranostics approaches in biological samples. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09476539_v24_n24_p6344_SamaniegoLopez http://hdl.handle.net/20.500.12110/paper_09476539_v24_n24_p6344_SamaniegoLopez |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
boronolectin cyanine dyes/pigments fluorescent probes glycoprotein Fluorescence Glycoproteins Infrared devices Boronolectin Cyanine Dyes/pigments Excellent solubility Fluorescent probes Fluorescent reporter Near infrared region Near-infrared emissions Probes boron derivative fluorescent dye glycoprotein mucin chemical structure chemistry drug effect epithelium cell human near infrared spectroscopy procedures Boron Compounds Epithelial Cells Fluorescent Dyes Glycoproteins Humans Molecular Structure Mucins Spectroscopy, Near-Infrared |
spellingShingle |
boronolectin cyanine dyes/pigments fluorescent probes glycoprotein Fluorescence Glycoproteins Infrared devices Boronolectin Cyanine Dyes/pigments Excellent solubility Fluorescent probes Fluorescent reporter Near infrared region Near-infrared emissions Probes boron derivative fluorescent dye glycoprotein mucin chemical structure chemistry drug effect epithelium cell human near infrared spectroscopy procedures Boron Compounds Epithelial Cells Fluorescent Dyes Glycoproteins Humans Molecular Structure Mucins Spectroscopy, Near-Infrared A Highly Sensitive Fluorogenic Probe for Imaging Glycoproteins and Mucine Activity in Live Cells in the Near-Infrared Region |
topic_facet |
boronolectin cyanine dyes/pigments fluorescent probes glycoprotein Fluorescence Glycoproteins Infrared devices Boronolectin Cyanine Dyes/pigments Excellent solubility Fluorescent probes Fluorescent reporter Near infrared region Near-infrared emissions Probes boron derivative fluorescent dye glycoprotein mucin chemical structure chemistry drug effect epithelium cell human near infrared spectroscopy procedures Boron Compounds Epithelial Cells Fluorescent Dyes Glycoproteins Humans Molecular Structure Mucins Spectroscopy, Near-Infrared |
description |
A novel fluorescent molecular probe is reported, which is able to detect glycoproteins, especially mucins, with high sensitivity and with a turn-on response along with a large Stokes shift (>130 nm), within the biologically active window. The probe contains an aminotricarbocyanine as the fluorescent reporter with a linked benzoboroxole as the recognition unit, which operates through a dynamic covalent reaction between the boronic hemiester residue of the receptor and cis-diols of the analyte. The superior selectivity of the probe is displayed by the labeling of mucins present in Calu-3 cells. The new benzoboroxole fluorescent derivative gathers together key properties to make it a highly rated molecular probe: specificity, excellent solubility in water, and off–on near infrared emission. This probe is expected to be an excellent tool for imaging intracellular mucin to evaluate mucus-related diseases as well as a sensing strategy towards glycosylated structures with a high potential for theranostics approaches in biological samples. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim |
title |
A Highly Sensitive Fluorogenic Probe for Imaging Glycoproteins and Mucine Activity in Live Cells in the Near-Infrared Region |
title_short |
A Highly Sensitive Fluorogenic Probe for Imaging Glycoproteins and Mucine Activity in Live Cells in the Near-Infrared Region |
title_full |
A Highly Sensitive Fluorogenic Probe for Imaging Glycoproteins and Mucine Activity in Live Cells in the Near-Infrared Region |
title_fullStr |
A Highly Sensitive Fluorogenic Probe for Imaging Glycoproteins and Mucine Activity in Live Cells in the Near-Infrared Region |
title_full_unstemmed |
A Highly Sensitive Fluorogenic Probe for Imaging Glycoproteins and Mucine Activity in Live Cells in the Near-Infrared Region |
title_sort |
highly sensitive fluorogenic probe for imaging glycoproteins and mucine activity in live cells in the near-infrared region |
publishDate |
2018 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09476539_v24_n24_p6344_SamaniegoLopez http://hdl.handle.net/20.500.12110/paper_09476539_v24_n24_p6344_SamaniegoLopez |
_version_ |
1768544874179592192 |