Two-Photon Excitation of a Plasmonic Nanoswitch Monitored by Single-Molecule Fluorescence Microscopy
Visible-light excitation of the surface plasmon band of silver nanoplates can effectively localize and concentrate the incident electromagnetic field enhancing the photochemical performance of organic molecules. Herein, the first single-molecule study of the plasmon-assisted isomerization of a photo...
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2016
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09476539_v22_n21_p7281_Impellizzeri http://hdl.handle.net/20.500.12110/paper_09476539_v22_n21_p7281_Impellizzeri |
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paper:paper_09476539_v22_n21_p7281_Impellizzeri2023-06-08T15:53:58Z Two-Photon Excitation of a Plasmonic Nanoswitch Monitored by Single-Molecule Fluorescence Microscopy Simoncelli, Sabrina Aramendía, Pedro Francisco nanoparticles photochromes surface plasmon TIRFM Chemical activation Electric excitation Electromagnetic fields Fluorescence Fluorescence microscopy Isomers Light Molecules Nanoparticles Nanostructures Photochromism Plasmons Refractive index Silver Stochastic systems Photochromes Photochromic transformations Single-molecule fluorescence microscopy Single-molecule studies Surface plasmons TIRFM Total internal reflection fluorescence microscopy Visible light excitation Laser excitation Visible-light excitation of the surface plasmon band of silver nanoplates can effectively localize and concentrate the incident electromagnetic field enhancing the photochemical performance of organic molecules. Herein, the first single-molecule study of the plasmon-assisted isomerization of a photochrome-fluorophore dyad, designed to switch between a nonfluorescent and a fluorescent state in response to the photochromic transformation, is reported. The photochemistry of the switchable assembly, consisting of a photochromic benzooxazine chemically conjugated to a coumarin moiety, is examined in real time with total internal reflection fluorescence microscopy in the presence of silver nanoplates excited with a 633 nm laser. The metallic nanostructures significantly enhance the visible light-induced performance of the photoconversion, which normally requires ultraviolet excitation. The resulting ring-open isomer is strongly fluorescent and can also be excited at 633 nm. These stochastic emission events are used to monitor photochromic activation and show quadratic dependence on incident power. The utilization of a single laser wavelength for both photochromic activation and excitation effectively mimics a pseudo two-colours system. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Fil:Simoncelli, S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Aramendia, P.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09476539_v22_n21_p7281_Impellizzeri http://hdl.handle.net/20.500.12110/paper_09476539_v22_n21_p7281_Impellizzeri |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
nanoparticles photochromes surface plasmon TIRFM Chemical activation Electric excitation Electromagnetic fields Fluorescence Fluorescence microscopy Isomers Light Molecules Nanoparticles Nanostructures Photochromism Plasmons Refractive index Silver Stochastic systems Photochromes Photochromic transformations Single-molecule fluorescence microscopy Single-molecule studies Surface plasmons TIRFM Total internal reflection fluorescence microscopy Visible light excitation Laser excitation |
| spellingShingle |
nanoparticles photochromes surface plasmon TIRFM Chemical activation Electric excitation Electromagnetic fields Fluorescence Fluorescence microscopy Isomers Light Molecules Nanoparticles Nanostructures Photochromism Plasmons Refractive index Silver Stochastic systems Photochromes Photochromic transformations Single-molecule fluorescence microscopy Single-molecule studies Surface plasmons TIRFM Total internal reflection fluorescence microscopy Visible light excitation Laser excitation Simoncelli, Sabrina Aramendía, Pedro Francisco Two-Photon Excitation of a Plasmonic Nanoswitch Monitored by Single-Molecule Fluorescence Microscopy |
| topic_facet |
nanoparticles photochromes surface plasmon TIRFM Chemical activation Electric excitation Electromagnetic fields Fluorescence Fluorescence microscopy Isomers Light Molecules Nanoparticles Nanostructures Photochromism Plasmons Refractive index Silver Stochastic systems Photochromes Photochromic transformations Single-molecule fluorescence microscopy Single-molecule studies Surface plasmons TIRFM Total internal reflection fluorescence microscopy Visible light excitation Laser excitation |
| description |
Visible-light excitation of the surface plasmon band of silver nanoplates can effectively localize and concentrate the incident electromagnetic field enhancing the photochemical performance of organic molecules. Herein, the first single-molecule study of the plasmon-assisted isomerization of a photochrome-fluorophore dyad, designed to switch between a nonfluorescent and a fluorescent state in response to the photochromic transformation, is reported. The photochemistry of the switchable assembly, consisting of a photochromic benzooxazine chemically conjugated to a coumarin moiety, is examined in real time with total internal reflection fluorescence microscopy in the presence of silver nanoplates excited with a 633 nm laser. The metallic nanostructures significantly enhance the visible light-induced performance of the photoconversion, which normally requires ultraviolet excitation. The resulting ring-open isomer is strongly fluorescent and can also be excited at 633 nm. These stochastic emission events are used to monitor photochromic activation and show quadratic dependence on incident power. The utilization of a single laser wavelength for both photochromic activation and excitation effectively mimics a pseudo two-colours system. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
| author |
Simoncelli, Sabrina Aramendía, Pedro Francisco |
| author_facet |
Simoncelli, Sabrina Aramendía, Pedro Francisco |
| author_sort |
Simoncelli, Sabrina |
| title |
Two-Photon Excitation of a Plasmonic Nanoswitch Monitored by Single-Molecule Fluorescence Microscopy |
| title_short |
Two-Photon Excitation of a Plasmonic Nanoswitch Monitored by Single-Molecule Fluorescence Microscopy |
| title_full |
Two-Photon Excitation of a Plasmonic Nanoswitch Monitored by Single-Molecule Fluorescence Microscopy |
| title_fullStr |
Two-Photon Excitation of a Plasmonic Nanoswitch Monitored by Single-Molecule Fluorescence Microscopy |
| title_full_unstemmed |
Two-Photon Excitation of a Plasmonic Nanoswitch Monitored by Single-Molecule Fluorescence Microscopy |
| title_sort |
two-photon excitation of a plasmonic nanoswitch monitored by single-molecule fluorescence microscopy |
| publishDate |
2016 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09476539_v22_n21_p7281_Impellizzeri http://hdl.handle.net/20.500.12110/paper_09476539_v22_n21_p7281_Impellizzeri |
| work_keys_str_mv |
AT simoncellisabrina twophotonexcitationofaplasmonicnanoswitchmonitoredbysinglemoleculefluorescencemicroscopy AT aramendiapedrofrancisco twophotonexcitationofaplasmonicnanoswitchmonitoredbysinglemoleculefluorescencemicroscopy |
| _version_ |
1768546452498284544 |