Wheat seeds harbour bacterial endophytes with potential as plant growth promoters and biocontrol agents of Fusarium graminearum

The role of endophytic communities of seeds is still poorly characterised. The purpose of this work was to survey the presence of bacterial endophytes in the seeds of a commercial wheat cultivar widely sown in Argentina and to look for plant growth promotion features and biocontrol abilities against...

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Detalles Bibliográficos
Publicado:	2016
Materias:	Barley Bioassays Fusarium Paenibacillus Pantoea Wheat-endophytes Bacteriology Bioassay Fungi Grain (agricultural product) RNA Seed Biocontrol bacterium barley bioassay biocontrol agent biofilm chlorophyll cultivar endophyte fungus genetic analysis growth regulator inoculation seed siderophore wheat Argentina Bacteria (microorganisms) Gammaproteobacteria Gibberella zeae Hordeum Triticum aestivum bacterial DNA indoleacetic acid indoleacetic acid derivative phosphate phytohormone ribosome DNA RNA 16S antibiosis chemistry classification DNA sequence genetics growth, development and aging isolation and purification metabolism microbiology plant seed promoter region Antibiosis Bacteria Biofilms DNA, Bacterial DNA, Ribosomal Endophytes Indoleacetic Acids Phosphates Plant Growth Regulators Promoter Regions, Genetic RNA, Ribosomal, 16S Seeds Sequence Analysis, DNA Siderophores Triticum
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09445013_v186-187_n_p37_DiazHerrera http://hdl.handle.net/20.500.12110/paper_09445013_v186-187_n_p37_DiazHerrera
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_09445013_v186-187_n_p37_DiazHerrera
record_format	dspace
spelling	paper:paper_09445013_v186-187_n_p37_DiazHerrera2023-06-08T15:53:49Z Wheat seeds harbour bacterial endophytes with potential as plant growth promoters and biocontrol agents of Fusarium graminearum Barley Bioassays Fusarium Paenibacillus Pantoea Wheat-endophytes Bacteriology Bioassay Fungi Grain (agricultural product) RNA Seed Barley Fusarium Paenibacillus Pantoea Wheat-endophytes Biocontrol bacterium barley bioassay biocontrol agent biofilm chlorophyll cultivar endophyte fungus genetic analysis growth regulator inoculation seed siderophore wheat Argentina Bacteria (microorganisms) Fusarium Gammaproteobacteria Gibberella zeae Hordeum Paenibacillus Pantoea Triticum aestivum bacterial DNA indoleacetic acid indoleacetic acid derivative phosphate phytohormone ribosome DNA RNA 16S siderophore antibiosis Argentina bacterium biofilm chemistry classification DNA sequence endophyte Fusarium genetics growth, development and aging Hordeum isolation and purification metabolism microbiology plant seed promoter region wheat Antibiosis Argentina Bacteria Biofilms DNA, Bacterial DNA, Ribosomal Endophytes Fusarium Hordeum Indoleacetic Acids Phosphates Plant Growth Regulators Promoter Regions, Genetic RNA, Ribosomal, 16S Seeds Sequence Analysis, DNA Siderophores Triticum The role of endophytic communities of seeds is still poorly characterised. The purpose of this work was to survey the presence of bacterial endophytes in the seeds of a commercial wheat cultivar widely sown in Argentina and to look for plant growth promotion features and biocontrol abilities against Fusarium graminearum among them. Six isolates were obtained from wheat seeds following a culture-dependent protocol. Four isolates were assignated to Paenibacillus genus according to their 16S rRNA sequencing. The only gammaproteobacteria isolated, presumably an Enterobactereaceae of Pantoea genus, was particularly active as IAA and siderophore producer, and also solubilised phosphate and was the only one that grew on N-free medium. Several of these isolates demonstrated ability to restrain F. graminearum growth on dual culture and in a bioassay using barley and wheat kernels. An outstanding ability to form biofilm on an inert surface was corroborated for those Paenibacillus which displayed greater biocontrol of F. graminearum, and the inoculation with one of these isolates in combination with the Pantoea isolate resulted in greater chlorophyll content in barley seedlings. Our results show a significant ecological potential of some components of the wheat seed endophytic community. © 2016 Elsevier GmbH. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09445013_v186-187_n_p37_DiazHerrera http://hdl.handle.net/20.500.12110/paper_09445013_v186-187_n_p37_DiazHerrera
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Barley Bioassays Fusarium Paenibacillus Pantoea Wheat-endophytes Bacteriology Bioassay Fungi Grain (agricultural product) RNA Seed Barley Fusarium Paenibacillus Pantoea Wheat-endophytes Biocontrol bacterium barley bioassay biocontrol agent biofilm chlorophyll cultivar endophyte fungus genetic analysis growth regulator inoculation seed siderophore wheat Argentina Bacteria (microorganisms) Fusarium Gammaproteobacteria Gibberella zeae Hordeum Paenibacillus Pantoea Triticum aestivum bacterial DNA indoleacetic acid indoleacetic acid derivative phosphate phytohormone ribosome DNA RNA 16S siderophore antibiosis Argentina bacterium biofilm chemistry classification DNA sequence endophyte Fusarium genetics growth, development and aging Hordeum isolation and purification metabolism microbiology plant seed promoter region wheat Antibiosis Argentina Bacteria Biofilms DNA, Bacterial DNA, Ribosomal Endophytes Fusarium Hordeum Indoleacetic Acids Phosphates Plant Growth Regulators Promoter Regions, Genetic RNA, Ribosomal, 16S Seeds Sequence Analysis, DNA Siderophores Triticum
spellingShingle	Barley Bioassays Fusarium Paenibacillus Pantoea Wheat-endophytes Bacteriology Bioassay Fungi Grain (agricultural product) RNA Seed Barley Fusarium Paenibacillus Pantoea Wheat-endophytes Biocontrol bacterium barley bioassay biocontrol agent biofilm chlorophyll cultivar endophyte fungus genetic analysis growth regulator inoculation seed siderophore wheat Argentina Bacteria (microorganisms) Fusarium Gammaproteobacteria Gibberella zeae Hordeum Paenibacillus Pantoea Triticum aestivum bacterial DNA indoleacetic acid indoleacetic acid derivative phosphate phytohormone ribosome DNA RNA 16S siderophore antibiosis Argentina bacterium biofilm chemistry classification DNA sequence endophyte Fusarium genetics growth, development and aging Hordeum isolation and purification metabolism microbiology plant seed promoter region wheat Antibiosis Argentina Bacteria Biofilms DNA, Bacterial DNA, Ribosomal Endophytes Fusarium Hordeum Indoleacetic Acids Phosphates Plant Growth Regulators Promoter Regions, Genetic RNA, Ribosomal, 16S Seeds Sequence Analysis, DNA Siderophores Triticum Wheat seeds harbour bacterial endophytes with potential as plant growth promoters and biocontrol agents of Fusarium graminearum
topic_facet	Barley Bioassays Fusarium Paenibacillus Pantoea Wheat-endophytes Bacteriology Bioassay Fungi Grain (agricultural product) RNA Seed Barley Fusarium Paenibacillus Pantoea Wheat-endophytes Biocontrol bacterium barley bioassay biocontrol agent biofilm chlorophyll cultivar endophyte fungus genetic analysis growth regulator inoculation seed siderophore wheat Argentina Bacteria (microorganisms) Fusarium Gammaproteobacteria Gibberella zeae Hordeum Paenibacillus Pantoea Triticum aestivum bacterial DNA indoleacetic acid indoleacetic acid derivative phosphate phytohormone ribosome DNA RNA 16S siderophore antibiosis Argentina bacterium biofilm chemistry classification DNA sequence endophyte Fusarium genetics growth, development and aging Hordeum isolation and purification metabolism microbiology plant seed promoter region wheat Antibiosis Argentina Bacteria Biofilms DNA, Bacterial DNA, Ribosomal Endophytes Fusarium Hordeum Indoleacetic Acids Phosphates Plant Growth Regulators Promoter Regions, Genetic RNA, Ribosomal, 16S Seeds Sequence Analysis, DNA Siderophores Triticum
description	The role of endophytic communities of seeds is still poorly characterised. The purpose of this work was to survey the presence of bacterial endophytes in the seeds of a commercial wheat cultivar widely sown in Argentina and to look for plant growth promotion features and biocontrol abilities against Fusarium graminearum among them. Six isolates were obtained from wheat seeds following a culture-dependent protocol. Four isolates were assignated to Paenibacillus genus according to their 16S rRNA sequencing. The only gammaproteobacteria isolated, presumably an Enterobactereaceae of Pantoea genus, was particularly active as IAA and siderophore producer, and also solubilised phosphate and was the only one that grew on N-free medium. Several of these isolates demonstrated ability to restrain F. graminearum growth on dual culture and in a bioassay using barley and wheat kernels. An outstanding ability to form biofilm on an inert surface was corroborated for those Paenibacillus which displayed greater biocontrol of F. graminearum, and the inoculation with one of these isolates in combination with the Pantoea isolate resulted in greater chlorophyll content in barley seedlings. Our results show a significant ecological potential of some components of the wheat seed endophytic community. © 2016 Elsevier GmbH.
title	Wheat seeds harbour bacterial endophytes with potential as plant growth promoters and biocontrol agents of Fusarium graminearum
title_short	Wheat seeds harbour bacterial endophytes with potential as plant growth promoters and biocontrol agents of Fusarium graminearum
title_full	Wheat seeds harbour bacterial endophytes with potential as plant growth promoters and biocontrol agents of Fusarium graminearum
title_fullStr	Wheat seeds harbour bacterial endophytes with potential as plant growth promoters and biocontrol agents of Fusarium graminearum
title_full_unstemmed	Wheat seeds harbour bacterial endophytes with potential as plant growth promoters and biocontrol agents of Fusarium graminearum
title_sort	wheat seeds harbour bacterial endophytes with potential as plant growth promoters and biocontrol agents of fusarium graminearum
publishDate	2016
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09445013_v186-187_n_p37_DiazHerrera http://hdl.handle.net/20.500.12110/paper_09445013_v186-187_n_p37_DiazHerrera
_version_	1768543570478759936

Wheat seeds harbour bacterial endophytes with potential as plant growth promoters and biocontrol agents of Fusarium graminearum

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