Antagonistic effects of Bacillus subtilis subsp. subtilis and B. amyloliquefaciens against Macrophomina phaseolina: SEM study of fungal changes and UV-MALDI-TOF MS analysis of their bioactive compounds

The antifungal effect of Bacillus subtilis subsp. subtilis PGPMori7 and Bacillus amyloliquefaciens PGPBacCA1 was evaluated against Macrophomina phaseolina (Tassi) Goid. Cell suspension (CS), cell-free supernatant (CFS) and the lipopeptide fraction (LF) of PGPMori7 and PGPBacCA1 were screened against...

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Detalles Bibliográficos
Autor principal: Erra Balsells, Rosa
Publicado: 2016
Materias:
B. amyloliquefaciens
Bacillus subtilis subsp. subtilis
Lipopeptides
Macrophomina phaseolina
SEM
UV-MALDI TOF MS
Bacteriology
Cell culture
Chemical analysis
Inductively coupled plasma
Metabolites
Scanning electron microscopy
Synthesis (chemical)
MALDI TOF MS
Subtilis
Fungi
antagonism
bacterium
fungus
inhibition
lipid
metabolite
microbial activity
pathogen
peptide
scanning electron microscopy
Bacilli (class)
Bacillus (bacterium)
Bacillus amyloliquefaciens
Bacteria (microorganisms)
Macrophomina
antifungal agent
Ascomycetes
Bacillus
Bacillus subtilis
chemistry
drug effects
growth, development and aging
matrix-assisted laser desorption-ionization mass spectrometry
metabolism
mycelium
ultrastructure
Antifungal Agents
Ascomycota
Microscopy, Electron, Scanning
Mycelium
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09445013_v182_n_p31_Torres
http://hdl.handle.net/20.500.12110/paper_09445013_v182_n_p31_Torres
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_09445013_v182_n_p31_Torres
record_format dspace
spelling paper:paper_09445013_v182_n_p31_Torres2023-06-08T15:53:48Z Antagonistic effects of Bacillus subtilis subsp. subtilis and B. amyloliquefaciens against Macrophomina phaseolina: SEM study of fungal changes and UV-MALDI-TOF MS analysis of their bioactive compounds Erra Balsells, Rosa B. amyloliquefaciens Bacillus subtilis subsp. subtilis Lipopeptides Macrophomina phaseolina SEM UV-MALDI TOF MS Bacteriology Cell culture Chemical analysis Inductively coupled plasma Metabolites Scanning electron microscopy Synthesis (chemical) B. amyloliquefaciens Lipopeptides Macrophomina phaseolina MALDI TOF MS Subtilis Fungi antagonism bacterium fungus inhibition lipid metabolite microbial activity pathogen peptide scanning electron microscopy Bacilli (class) Bacillus (bacterium) Bacillus amyloliquefaciens Bacillus subtilis subsp. subtilis Bacteria (microorganisms) Fungi Macrophomina Macrophomina phaseolina antifungal agent Ascomycetes Bacillus Bacillus subtilis chemistry drug effects growth, development and aging matrix-assisted laser desorption-ionization mass spectrometry metabolism mycelium scanning electron microscopy ultrastructure Antifungal Agents Ascomycota Bacillus Bacillus subtilis Microscopy, Electron, Scanning Mycelium Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization The antifungal effect of Bacillus subtilis subsp. subtilis PGPMori7 and Bacillus amyloliquefaciens PGPBacCA1 was evaluated against Macrophomina phaseolina (Tassi) Goid. Cell suspension (CS), cell-free supernatant (CFS) and the lipopeptide fraction (LF) of PGPMori7 and PGPBacCA1 were screened against three different M. phaseolina strains. CS exhibited the highest inhibitory effect (around 50%) when compared to those of CFS and LF, regardless of the fungal strain studied. The synthesis of lipopeptides was studied by UV-MALDI TOF. Chemical analysis of Bacillus metabolite synthesis revealed that surfactin and iturin were mainly produced in liquid medium. Potential fengycin was also co-produced when both Bacillus were cultivated in solid medium. In co-culture assays, the bacterial colony-fungal mycelium interface at the inhibition zone was evaluated by both scanning electron microscopy (SEM) and UV-MALDI TOF, the former to determine the structural changes on M. phaseolina cells and the latter to identify the main bioactive molecules involved in the inhibitory effect. PGPBacCA1 produced surfactin, iturin and fengycin in the inhibition zone while PGPMori7 only produced these metabolites within its colony and not in the narrow inhibition zone. Interestingly, SEM revealed that PGPBacCA1 induced damage in M. phaseolina sclerotia, generating a fungicidal effect as no growth was observed when normal growth conditions were reestablished. In turn, PGPMori7 inhibited the growth of the Macrophomina mycelium without fungal injury, resulting only in a fungistatic activity. From these results, it was determined that the two bacilli significantly inhibited the growth of an important phytopathogenic fungus by at least two different mechanisms: lipopeptide synthesis and competition among microorganisms. © 2015 Elsevier GmbH. Fil:Erra-Balsells, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09445013_v182_n_p31_Torres http://hdl.handle.net/20.500.12110/paper_09445013_v182_n_p31_Torres
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic B. amyloliquefaciens
Bacillus subtilis subsp. subtilis
Lipopeptides
Macrophomina phaseolina
SEM
UV-MALDI TOF MS
Bacteriology
Cell culture
Chemical analysis
Inductively coupled plasma
Metabolites
Scanning electron microscopy
Synthesis (chemical)
B. amyloliquefaciens
Lipopeptides
Macrophomina phaseolina
MALDI TOF MS
Subtilis
Fungi
antagonism
bacterium
fungus
inhibition
lipid
metabolite
microbial activity
pathogen
peptide
scanning electron microscopy
Bacilli (class)
Bacillus (bacterium)
Bacillus amyloliquefaciens
Bacillus subtilis subsp. subtilis
Bacteria (microorganisms)
Fungi
Macrophomina
Macrophomina phaseolina
antifungal agent
Ascomycetes
Bacillus
Bacillus subtilis
chemistry
drug effects
growth, development and aging
matrix-assisted laser desorption-ionization mass spectrometry
metabolism
mycelium
scanning electron microscopy
ultrastructure
Antifungal Agents
Ascomycota
Bacillus
Bacillus subtilis
Microscopy, Electron, Scanning
Mycelium
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
spellingShingle B. amyloliquefaciens
Bacillus subtilis subsp. subtilis
Lipopeptides
Macrophomina phaseolina
SEM
UV-MALDI TOF MS
Bacteriology
Cell culture
Chemical analysis
Inductively coupled plasma
Metabolites
Scanning electron microscopy
Synthesis (chemical)
B. amyloliquefaciens
Lipopeptides
Macrophomina phaseolina
MALDI TOF MS
Subtilis
Fungi
antagonism
bacterium
fungus
inhibition
lipid
metabolite
microbial activity
pathogen
peptide
scanning electron microscopy
Bacilli (class)
Bacillus (bacterium)
Bacillus amyloliquefaciens
Bacillus subtilis subsp. subtilis
Bacteria (microorganisms)
Fungi
Macrophomina
Macrophomina phaseolina
antifungal agent
Ascomycetes
Bacillus
Bacillus subtilis
chemistry
drug effects
growth, development and aging
matrix-assisted laser desorption-ionization mass spectrometry
metabolism
mycelium
scanning electron microscopy
ultrastructure
Antifungal Agents
Ascomycota
Bacillus
Bacillus subtilis
Microscopy, Electron, Scanning
Mycelium
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Erra Balsells, Rosa
Antagonistic effects of Bacillus subtilis subsp. subtilis and B. amyloliquefaciens against Macrophomina phaseolina: SEM study of fungal changes and UV-MALDI-TOF MS analysis of their bioactive compounds
topic_facet B. amyloliquefaciens
Bacillus subtilis subsp. subtilis
Lipopeptides
Macrophomina phaseolina
SEM
UV-MALDI TOF MS
Bacteriology
Cell culture
Chemical analysis
Inductively coupled plasma
Metabolites
Scanning electron microscopy
Synthesis (chemical)
B. amyloliquefaciens
Lipopeptides
Macrophomina phaseolina
MALDI TOF MS
Subtilis
Fungi
antagonism
bacterium
fungus
inhibition
lipid
metabolite
microbial activity
pathogen
peptide
scanning electron microscopy
Bacilli (class)
Bacillus (bacterium)
Bacillus amyloliquefaciens
Bacillus subtilis subsp. subtilis
Bacteria (microorganisms)
Fungi
Macrophomina
Macrophomina phaseolina
antifungal agent
Ascomycetes
Bacillus
Bacillus subtilis
chemistry
drug effects
growth, development and aging
matrix-assisted laser desorption-ionization mass spectrometry
metabolism
mycelium
scanning electron microscopy
ultrastructure
Antifungal Agents
Ascomycota
Bacillus
Bacillus subtilis
Microscopy, Electron, Scanning
Mycelium
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
description The antifungal effect of Bacillus subtilis subsp. subtilis PGPMori7 and Bacillus amyloliquefaciens PGPBacCA1 was evaluated against Macrophomina phaseolina (Tassi) Goid. Cell suspension (CS), cell-free supernatant (CFS) and the lipopeptide fraction (LF) of PGPMori7 and PGPBacCA1 were screened against three different M. phaseolina strains. CS exhibited the highest inhibitory effect (around 50%) when compared to those of CFS and LF, regardless of the fungal strain studied. The synthesis of lipopeptides was studied by UV-MALDI TOF. Chemical analysis of Bacillus metabolite synthesis revealed that surfactin and iturin were mainly produced in liquid medium. Potential fengycin was also co-produced when both Bacillus were cultivated in solid medium. In co-culture assays, the bacterial colony-fungal mycelium interface at the inhibition zone was evaluated by both scanning electron microscopy (SEM) and UV-MALDI TOF, the former to determine the structural changes on M. phaseolina cells and the latter to identify the main bioactive molecules involved in the inhibitory effect. PGPBacCA1 produced surfactin, iturin and fengycin in the inhibition zone while PGPMori7 only produced these metabolites within its colony and not in the narrow inhibition zone. Interestingly, SEM revealed that PGPBacCA1 induced damage in M. phaseolina sclerotia, generating a fungicidal effect as no growth was observed when normal growth conditions were reestablished. In turn, PGPMori7 inhibited the growth of the Macrophomina mycelium without fungal injury, resulting only in a fungistatic activity. From these results, it was determined that the two bacilli significantly inhibited the growth of an important phytopathogenic fungus by at least two different mechanisms: lipopeptide synthesis and competition among microorganisms. © 2015 Elsevier GmbH.
author Erra Balsells, Rosa
author_facet Erra Balsells, Rosa
author_sort Erra Balsells, Rosa
title Antagonistic effects of Bacillus subtilis subsp. subtilis and B. amyloliquefaciens against Macrophomina phaseolina: SEM study of fungal changes and UV-MALDI-TOF MS analysis of their bioactive compounds
title_short Antagonistic effects of Bacillus subtilis subsp. subtilis and B. amyloliquefaciens against Macrophomina phaseolina: SEM study of fungal changes and UV-MALDI-TOF MS analysis of their bioactive compounds
title_full Antagonistic effects of Bacillus subtilis subsp. subtilis and B. amyloliquefaciens against Macrophomina phaseolina: SEM study of fungal changes and UV-MALDI-TOF MS analysis of their bioactive compounds
title_fullStr Antagonistic effects of Bacillus subtilis subsp. subtilis and B. amyloliquefaciens against Macrophomina phaseolina: SEM study of fungal changes and UV-MALDI-TOF MS analysis of their bioactive compounds
title_full_unstemmed Antagonistic effects of Bacillus subtilis subsp. subtilis and B. amyloliquefaciens against Macrophomina phaseolina: SEM study of fungal changes and UV-MALDI-TOF MS analysis of their bioactive compounds
title_sort antagonistic effects of bacillus subtilis subsp. subtilis and b. amyloliquefaciens against macrophomina phaseolina: sem study of fungal changes and uv-maldi-tof ms analysis of their bioactive compounds
publishDate 2016
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09445013_v182_n_p31_Torres
http://hdl.handle.net/20.500.12110/paper_09445013_v182_n_p31_Torres
work_keys_str_mv AT errabalsellsrosa antagonisticeffectsofbacillussubtilissubspsubtilisandbamyloliquefaciensagainstmacrophominaphaseolinasemstudyoffungalchangesanduvmalditofmsanalysisoftheirbioactivecompounds
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