Non-Essential Activation of Rat Liver Porphobilinogen-Deaminase by Folic Acid*
This report demonstrates the ability of folic acid to activate rat liver porphobilinogen-deaminase (PBG-D). Lineweaver-Burk analysis revealed an increase in Vmax (38%) without affecting the Km. In the concentration range assayed, secondary replots of 1/Aslope and 1/Aintersect versus 1/[folic acid] y...
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1992
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09395075_v47_n5-6_p416_Noriega http://hdl.handle.net/20.500.12110/paper_09395075_v47_n5-6_p416_Noriega |
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paper:paper_09395075_v47_n5-6_p416_Noriega2023-06-08T15:53:26Z Non-Essential Activation of Rat Liver Porphobilinogen-Deaminase by Folic Acid* Noriega, Guillermo Osvaldo Juknat, Adela Ana Batlle, Alcira María del Carmen Cosynthetase Folic Acid Nonessential Activation Porphobilinogen-Deaminase Uroporphyrinogen I folic acid porphobilinogen deaminase animal article cell fractionation cytosol enzyme activation enzymology isolation and purification kinetics liver male metabolism protein binding rat rat strain theoretical model Animal Cell Fractionation Cytosol Enzyme Activation Folic Acid Hydroxymethylbilane Synthase Kinetics Liver Male Models, Theoretical Protein Binding Rats Rats, Inbred Strains Support, Non-U.S. Gov't This report demonstrates the ability of folic acid to activate rat liver porphobilinogen-deaminase (PBG-D). Lineweaver-Burk analysis revealed an increase in Vmax (38%) without affecting the Km. In the concentration range assayed, secondary replots of 1/Aslope and 1/Aintersect versus 1/[folic acid] yielded straight lines, indicating the binding of a single molecule of activator to the enzyme PBG-D, with a KA = 1.66 mM. Results presented here show that folic acid acts as a non-essential activator (α = 1; ß= 1.6). The activating effect of folic acid has been observed employing the 35-70% ammonium sulphate precipitated fraction, desalted by dialysis or gel filtration, whereas no action was detected when other partially purified PBG-D preparations were utilized as the enzyme source, suggesting either the presence of sites saturated for the activator, or the existence of a different structural protein conformation, or both. © 1992, Walter de Gruyter. All rights reserved. Fil:Noriega, G.O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Juknat, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:del Batlle, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1992 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09395075_v47_n5-6_p416_Noriega http://hdl.handle.net/20.500.12110/paper_09395075_v47_n5-6_p416_Noriega |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cosynthetase Folic Acid Nonessential Activation Porphobilinogen-Deaminase Uroporphyrinogen I folic acid porphobilinogen deaminase animal article cell fractionation cytosol enzyme activation enzymology isolation and purification kinetics liver male metabolism protein binding rat rat strain theoretical model Animal Cell Fractionation Cytosol Enzyme Activation Folic Acid Hydroxymethylbilane Synthase Kinetics Liver Male Models, Theoretical Protein Binding Rats Rats, Inbred Strains Support, Non-U.S. Gov't |
| spellingShingle |
Cosynthetase Folic Acid Nonessential Activation Porphobilinogen-Deaminase Uroporphyrinogen I folic acid porphobilinogen deaminase animal article cell fractionation cytosol enzyme activation enzymology isolation and purification kinetics liver male metabolism protein binding rat rat strain theoretical model Animal Cell Fractionation Cytosol Enzyme Activation Folic Acid Hydroxymethylbilane Synthase Kinetics Liver Male Models, Theoretical Protein Binding Rats Rats, Inbred Strains Support, Non-U.S. Gov't Noriega, Guillermo Osvaldo Juknat, Adela Ana Batlle, Alcira María del Carmen Non-Essential Activation of Rat Liver Porphobilinogen-Deaminase by Folic Acid* |
| topic_facet |
Cosynthetase Folic Acid Nonessential Activation Porphobilinogen-Deaminase Uroporphyrinogen I folic acid porphobilinogen deaminase animal article cell fractionation cytosol enzyme activation enzymology isolation and purification kinetics liver male metabolism protein binding rat rat strain theoretical model Animal Cell Fractionation Cytosol Enzyme Activation Folic Acid Hydroxymethylbilane Synthase Kinetics Liver Male Models, Theoretical Protein Binding Rats Rats, Inbred Strains Support, Non-U.S. Gov't |
| description |
This report demonstrates the ability of folic acid to activate rat liver porphobilinogen-deaminase (PBG-D). Lineweaver-Burk analysis revealed an increase in Vmax (38%) without affecting the Km. In the concentration range assayed, secondary replots of 1/Aslope and 1/Aintersect versus 1/[folic acid] yielded straight lines, indicating the binding of a single molecule of activator to the enzyme PBG-D, with a KA = 1.66 mM. Results presented here show that folic acid acts as a non-essential activator (α = 1; ß= 1.6). The activating effect of folic acid has been observed employing the 35-70% ammonium sulphate precipitated fraction, desalted by dialysis or gel filtration, whereas no action was detected when other partially purified PBG-D preparations were utilized as the enzyme source, suggesting either the presence of sites saturated for the activator, or the existence of a different structural protein conformation, or both. © 1992, Walter de Gruyter. All rights reserved. |
| author |
Noriega, Guillermo Osvaldo Juknat, Adela Ana Batlle, Alcira María del Carmen |
| author_facet |
Noriega, Guillermo Osvaldo Juknat, Adela Ana Batlle, Alcira María del Carmen |
| author_sort |
Noriega, Guillermo Osvaldo |
| title |
Non-Essential Activation of Rat Liver Porphobilinogen-Deaminase by Folic Acid* |
| title_short |
Non-Essential Activation of Rat Liver Porphobilinogen-Deaminase by Folic Acid* |
| title_full |
Non-Essential Activation of Rat Liver Porphobilinogen-Deaminase by Folic Acid* |
| title_fullStr |
Non-Essential Activation of Rat Liver Porphobilinogen-Deaminase by Folic Acid* |
| title_full_unstemmed |
Non-Essential Activation of Rat Liver Porphobilinogen-Deaminase by Folic Acid* |
| title_sort |
non-essential activation of rat liver porphobilinogen-deaminase by folic acid* |
| publishDate |
1992 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09395075_v47_n5-6_p416_Noriega http://hdl.handle.net/20.500.12110/paper_09395075_v47_n5-6_p416_Noriega |
| work_keys_str_mv |
AT noriegaguillermoosvaldo nonessentialactivationofratliverporphobilinogendeaminasebyfolicacid AT juknatadelaana nonessentialactivationofratliverporphobilinogendeaminasebyfolicacid AT batllealciramariadelcarmen nonessentialactivationofratliverporphobilinogendeaminasebyfolicacid |
| _version_ |
1768542184321056768 |