Anthelmintic effect of Mentha spp. essential oils on Echinococcus granulosus protoscoleces and metacestodes
The aim of the present work was to determine the in vitro effect of Mentha piperita and Mentha pulegium essential oils against Echinococcus granulosus and to compare the effectiveness of both oils according to the exposure time and concentration. Although both treatments had a protoscolicidal effect...
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2012
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09320113_v110_n3_p1103_Maggiore http://hdl.handle.net/20.500.12110/paper_09320113_v110_n3_p1103_Maggiore |
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paper:paper_09320113_v110_n3_p1103_Maggiore2023-06-08T15:53:00Z Anthelmintic effect of Mentha spp. essential oils on Echinococcus granulosus protoscoleces and metacestodes anthelmintic agent essential oil vegetable oil Mentha piperita essential oil Mentha pulegium essential oil unclassified drug animal article chemistry comparative study dose response drug effect drug sensitivity echinococcosis Echinococcus granulosus female growth, development and aging Mentha piperita Mentha pulegium mouse parasitology pathogenicity phytotherapy scanning electron microscopy species difference survival time transmission electron microscopy ultrastructure cestode life cycle stage concentration response controlled study cyst (resting stage) in vitro study metacestode nonhuman parasite survival physical chemistry plant leaf priority journal protoscole Animals Anthelmintics Dose-Response Relationship, Drug Echinococcosis Echinococcus granulosus Female Mentha piperita Mentha pulegium Mice Microscopy, Electron, Scanning Microscopy, Electron, Transmission Oils, Volatile Parasitic Sensitivity Tests Phytotherapy Plant Oils Species Specificity Survival Analysis Time Factors Echinococcus granulosus Mentha Mentha pulegium Mentha x piperita Mus The aim of the present work was to determine the in vitro effect of Mentha piperita and Mentha pulegium essential oils against Echinococcus granulosus and to compare the effectiveness of both oils according to the exposure time and concentration. Although both treatments had a protoscolicidal effect, M. pulegium had a considerably stronger effect than M. piperita. Essential oil of M. pulegium produced dose- and time-dependent effects. Maximal protoscolicidal effect was observed after 12 days of incubation and reached 0% after 18 days. This lack of viability was proved during the determination of infectivity into mice. Essential oil of M. piperita produced only a time-dependent effect. At 24 days p.i., the viability of protoscoleces decreased to approximately 50%. Scanning and transmission electron microscopy (SEM and TEM) demonstrated the drug-induced ultrastructural damage. On the other hand, a loss of turgidity was detected in all M. pulegium-treated cysts respective of the drug concentration. There was a correlation between the intensity of damage and the concentration of the essential oil assayed. Studies by SEM revealed that the germinal layer of treated cysts lost the feature multicellular structure. M. pulegium essential oil showed piperitone oxide as main compound in their composition, and we suggest that this component could be responsible of the markedly anthelmintic effect detected. Our data suggest that essential oils of Mentha spp. can be a promising source of potential protoscolicidal agents. The isolation of active anthelmintic constituents is in progress and may lead to the discovery of compounds with improved therapeutic value. © Springer-Verlag 2011. 2012 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09320113_v110_n3_p1103_Maggiore http://hdl.handle.net/20.500.12110/paper_09320113_v110_n3_p1103_Maggiore |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
anthelmintic agent essential oil vegetable oil Mentha piperita essential oil Mentha pulegium essential oil unclassified drug animal article chemistry comparative study dose response drug effect drug sensitivity echinococcosis Echinococcus granulosus female growth, development and aging Mentha piperita Mentha pulegium mouse parasitology pathogenicity phytotherapy scanning electron microscopy species difference survival time transmission electron microscopy ultrastructure cestode life cycle stage concentration response controlled study cyst (resting stage) in vitro study metacestode nonhuman parasite survival physical chemistry plant leaf priority journal protoscole Animals Anthelmintics Dose-Response Relationship, Drug Echinococcosis Echinococcus granulosus Female Mentha piperita Mentha pulegium Mice Microscopy, Electron, Scanning Microscopy, Electron, Transmission Oils, Volatile Parasitic Sensitivity Tests Phytotherapy Plant Oils Species Specificity Survival Analysis Time Factors Echinococcus granulosus Mentha Mentha pulegium Mentha x piperita Mus |
| spellingShingle |
anthelmintic agent essential oil vegetable oil Mentha piperita essential oil Mentha pulegium essential oil unclassified drug animal article chemistry comparative study dose response drug effect drug sensitivity echinococcosis Echinococcus granulosus female growth, development and aging Mentha piperita Mentha pulegium mouse parasitology pathogenicity phytotherapy scanning electron microscopy species difference survival time transmission electron microscopy ultrastructure cestode life cycle stage concentration response controlled study cyst (resting stage) in vitro study metacestode nonhuman parasite survival physical chemistry plant leaf priority journal protoscole Animals Anthelmintics Dose-Response Relationship, Drug Echinococcosis Echinococcus granulosus Female Mentha piperita Mentha pulegium Mice Microscopy, Electron, Scanning Microscopy, Electron, Transmission Oils, Volatile Parasitic Sensitivity Tests Phytotherapy Plant Oils Species Specificity Survival Analysis Time Factors Echinococcus granulosus Mentha Mentha pulegium Mentha x piperita Mus Anthelmintic effect of Mentha spp. essential oils on Echinococcus granulosus protoscoleces and metacestodes |
| topic_facet |
anthelmintic agent essential oil vegetable oil Mentha piperita essential oil Mentha pulegium essential oil unclassified drug animal article chemistry comparative study dose response drug effect drug sensitivity echinococcosis Echinococcus granulosus female growth, development and aging Mentha piperita Mentha pulegium mouse parasitology pathogenicity phytotherapy scanning electron microscopy species difference survival time transmission electron microscopy ultrastructure cestode life cycle stage concentration response controlled study cyst (resting stage) in vitro study metacestode nonhuman parasite survival physical chemistry plant leaf priority journal protoscole Animals Anthelmintics Dose-Response Relationship, Drug Echinococcosis Echinococcus granulosus Female Mentha piperita Mentha pulegium Mice Microscopy, Electron, Scanning Microscopy, Electron, Transmission Oils, Volatile Parasitic Sensitivity Tests Phytotherapy Plant Oils Species Specificity Survival Analysis Time Factors Echinococcus granulosus Mentha Mentha pulegium Mentha x piperita Mus |
| description |
The aim of the present work was to determine the in vitro effect of Mentha piperita and Mentha pulegium essential oils against Echinococcus granulosus and to compare the effectiveness of both oils according to the exposure time and concentration. Although both treatments had a protoscolicidal effect, M. pulegium had a considerably stronger effect than M. piperita. Essential oil of M. pulegium produced dose- and time-dependent effects. Maximal protoscolicidal effect was observed after 12 days of incubation and reached 0% after 18 days. This lack of viability was proved during the determination of infectivity into mice. Essential oil of M. piperita produced only a time-dependent effect. At 24 days p.i., the viability of protoscoleces decreased to approximately 50%. Scanning and transmission electron microscopy (SEM and TEM) demonstrated the drug-induced ultrastructural damage. On the other hand, a loss of turgidity was detected in all M. pulegium-treated cysts respective of the drug concentration. There was a correlation between the intensity of damage and the concentration of the essential oil assayed. Studies by SEM revealed that the germinal layer of treated cysts lost the feature multicellular structure. M. pulegium essential oil showed piperitone oxide as main compound in their composition, and we suggest that this component could be responsible of the markedly anthelmintic effect detected. Our data suggest that essential oils of Mentha spp. can be a promising source of potential protoscolicidal agents. The isolation of active anthelmintic constituents is in progress and may lead to the discovery of compounds with improved therapeutic value. © Springer-Verlag 2011. |
| title |
Anthelmintic effect of Mentha spp. essential oils on Echinococcus granulosus protoscoleces and metacestodes |
| title_short |
Anthelmintic effect of Mentha spp. essential oils on Echinococcus granulosus protoscoleces and metacestodes |
| title_full |
Anthelmintic effect of Mentha spp. essential oils on Echinococcus granulosus protoscoleces and metacestodes |
| title_fullStr |
Anthelmintic effect of Mentha spp. essential oils on Echinococcus granulosus protoscoleces and metacestodes |
| title_full_unstemmed |
Anthelmintic effect of Mentha spp. essential oils on Echinococcus granulosus protoscoleces and metacestodes |
| title_sort |
anthelmintic effect of mentha spp. essential oils on echinococcus granulosus protoscoleces and metacestodes |
| publishDate |
2012 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09320113_v110_n3_p1103_Maggiore http://hdl.handle.net/20.500.12110/paper_09320113_v110_n3_p1103_Maggiore |
| _version_ |
1768543189333966848 |