PCR detection of Dirofilaria immitis in Aedes aegypti and Culex pipiens from urban temperate Argentina

Dirofilariasis, a mosquito-borne disease of dogs caused by the nematode Dirofilaria immitis (Leidy; Spirurida: Onchocercidae), has now become a growing zoonotic concern. Based on direct microscopical observation, Aedes aegypti (L.) and Culex pipiens L. (Diptera: Culicidae) have been previously incri...

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Autor principal:	Vezzani, Dario
Publicado:	2011
Materias:	RNA 16S Aedes aegypti animal tissue Argentina arthropod larva article controlled study Culex pipiens Dirofilaria immitis gene amplification mosquito nonhuman nucleotide sequence Ochlerotatus parasite identification polymerase chain reaction priority journal Psorophora reliability urban area Aedes Animals Culex DNA Primers DNA, Helminth Molecular Sequence Data Parasitology Polymerase Chain Reaction RNA, Helminth RNA, Ribosomal, 16S Sequence Analysis, DNA Canis familiaris Culicidae Diptera Onchocercidae Spirurida
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09320113_v108_n4_p985_Vezzani http://hdl.handle.net/20.500.12110/paper_09320113_v108_n4_p985_Vezzani
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_09320113_v108_n4_p985_Vezzani
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spelling	paper:paper_09320113_v108_n4_p985_Vezzani2023-06-08T15:52:59Z PCR detection of Dirofilaria immitis in Aedes aegypti and Culex pipiens from urban temperate Argentina Vezzani, Dario RNA 16S Aedes aegypti animal tissue Argentina arthropod larva article controlled study Culex pipiens Dirofilaria immitis gene amplification mosquito nonhuman nucleotide sequence Ochlerotatus parasite identification polymerase chain reaction priority journal Psorophora reliability urban area Aedes Animals Argentina Culex Dirofilaria immitis DNA Primers DNA, Helminth Molecular Sequence Data Parasitology Polymerase Chain Reaction RNA, Helminth RNA, Ribosomal, 16S Sequence Analysis, DNA Aedes aegypti Canis familiaris Culex pipiens Culicidae Diptera Dirofilaria immitis Onchocercidae Psorophora Spirurida Dirofilariasis, a mosquito-borne disease of dogs caused by the nematode Dirofilaria immitis (Leidy; Spirurida: Onchocercidae), has now become a growing zoonotic concern. Based on direct microscopical observation, Aedes aegypti (L.) and Culex pipiens L. (Diptera: Culicidae) have been previously incriminated as potential vectors of D. immitis in urban temperate Argentina. In this study, an effort was made to provide evidence for this assumption by screening of mosquitoes for D. immitis infection using a polymerase chain reaction (PCR) assay. PCR primers were developed to specifically amplify the D. immitis-16S rRNA gene and to reliably detect 100th of the genomic equivalent (10 pg) of the infective third-stage larvae in mosquito pools of up to 30 individuals. Collection of mosquitoes was performed between September 2007 and April 2008 in premises known to be inhabited by D. immitis-infected dogs in Greater Buenos Aires. The final collection comprised 453 specimens belonging to 11 mosquito species of the genera Aedes, Culex, Ochlerotatus, and Psorophora. PCR assays were performed on 82 pools (n≤20) of heads and abdomens separately, as this allows differentiating infective and non-infective stages of the parasite, respectively. Identification of the non-infective stage of D. immitis in A. aegypti and C. pipiens provided additional strong support of transmission of the parasite by these species. To our knowledge, this was the first PCR screening for D. immitis-infected mosquitoes in South America. © Springer-Verlag 2010. Fil:Vezzani, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09320113_v108_n4_p985_Vezzani http://hdl.handle.net/20.500.12110/paper_09320113_v108_n4_p985_Vezzani
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	RNA 16S Aedes aegypti animal tissue Argentina arthropod larva article controlled study Culex pipiens Dirofilaria immitis gene amplification mosquito nonhuman nucleotide sequence Ochlerotatus parasite identification polymerase chain reaction priority journal Psorophora reliability urban area Aedes Animals Argentina Culex Dirofilaria immitis DNA Primers DNA, Helminth Molecular Sequence Data Parasitology Polymerase Chain Reaction RNA, Helminth RNA, Ribosomal, 16S Sequence Analysis, DNA Aedes aegypti Canis familiaris Culex pipiens Culicidae Diptera Dirofilaria immitis Onchocercidae Psorophora Spirurida
spellingShingle	RNA 16S Aedes aegypti animal tissue Argentina arthropod larva article controlled study Culex pipiens Dirofilaria immitis gene amplification mosquito nonhuman nucleotide sequence Ochlerotatus parasite identification polymerase chain reaction priority journal Psorophora reliability urban area Aedes Animals Argentina Culex Dirofilaria immitis DNA Primers DNA, Helminth Molecular Sequence Data Parasitology Polymerase Chain Reaction RNA, Helminth RNA, Ribosomal, 16S Sequence Analysis, DNA Aedes aegypti Canis familiaris Culex pipiens Culicidae Diptera Dirofilaria immitis Onchocercidae Psorophora Spirurida Vezzani, Dario PCR detection of Dirofilaria immitis in Aedes aegypti and Culex pipiens from urban temperate Argentina
topic_facet	RNA 16S Aedes aegypti animal tissue Argentina arthropod larva article controlled study Culex pipiens Dirofilaria immitis gene amplification mosquito nonhuman nucleotide sequence Ochlerotatus parasite identification polymerase chain reaction priority journal Psorophora reliability urban area Aedes Animals Argentina Culex Dirofilaria immitis DNA Primers DNA, Helminth Molecular Sequence Data Parasitology Polymerase Chain Reaction RNA, Helminth RNA, Ribosomal, 16S Sequence Analysis, DNA Aedes aegypti Canis familiaris Culex pipiens Culicidae Diptera Dirofilaria immitis Onchocercidae Psorophora Spirurida
description	Dirofilariasis, a mosquito-borne disease of dogs caused by the nematode Dirofilaria immitis (Leidy; Spirurida: Onchocercidae), has now become a growing zoonotic concern. Based on direct microscopical observation, Aedes aegypti (L.) and Culex pipiens L. (Diptera: Culicidae) have been previously incriminated as potential vectors of D. immitis in urban temperate Argentina. In this study, an effort was made to provide evidence for this assumption by screening of mosquitoes for D. immitis infection using a polymerase chain reaction (PCR) assay. PCR primers were developed to specifically amplify the D. immitis-16S rRNA gene and to reliably detect 100th of the genomic equivalent (10 pg) of the infective third-stage larvae in mosquito pools of up to 30 individuals. Collection of mosquitoes was performed between September 2007 and April 2008 in premises known to be inhabited by D. immitis-infected dogs in Greater Buenos Aires. The final collection comprised 453 specimens belonging to 11 mosquito species of the genera Aedes, Culex, Ochlerotatus, and Psorophora. PCR assays were performed on 82 pools (n≤20) of heads and abdomens separately, as this allows differentiating infective and non-infective stages of the parasite, respectively. Identification of the non-infective stage of D. immitis in A. aegypti and C. pipiens provided additional strong support of transmission of the parasite by these species. To our knowledge, this was the first PCR screening for D. immitis-infected mosquitoes in South America. © Springer-Verlag 2010.
author	Vezzani, Dario
author_facet	Vezzani, Dario
author_sort	Vezzani, Dario
title	PCR detection of Dirofilaria immitis in Aedes aegypti and Culex pipiens from urban temperate Argentina
title_short	PCR detection of Dirofilaria immitis in Aedes aegypti and Culex pipiens from urban temperate Argentina
title_full	PCR detection of Dirofilaria immitis in Aedes aegypti and Culex pipiens from urban temperate Argentina
title_fullStr	PCR detection of Dirofilaria immitis in Aedes aegypti and Culex pipiens from urban temperate Argentina
title_full_unstemmed	PCR detection of Dirofilaria immitis in Aedes aegypti and Culex pipiens from urban temperate Argentina
title_sort	pcr detection of dirofilaria immitis in aedes aegypti and culex pipiens from urban temperate argentina
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09320113_v108_n4_p985_Vezzani http://hdl.handle.net/20.500.12110/paper_09320113_v108_n4_p985_Vezzani
work_keys_str_mv	AT vezzanidario pcrdetectionofdirofilariaimmitisinaedesaegyptiandculexpipiensfromurbantemperateargentina
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PCR detection of Dirofilaria immitis in Aedes aegypti and Culex pipiens from urban temperate Argentina

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