Effect of bacterial metabolites on microsporidian Nosema ceranae and on its host Apis mellifera
Nosemosis, a disease caused by a microsporidian infection, is one of the most frequently observed parasitic pathologies affecting adult honeybees. Presently, Nosema ceranae seems to be the main microsporidian infection in Apis mellifera. The antibiotic fumagillin is the only compound available to tr...
Guardado en:
| Publicado: |
2010
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09320113_v107_n2_p381_Porrini http://hdl.handle.net/20.500.12110/paper_09320113_v107_n2_p381_Porrini |
| Aporte de: |
| id |
paper:paper_09320113_v107_n2_p381_Porrini |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_09320113_v107_n2_p381_Porrini2023-06-08T15:52:59Z Effect of bacterial metabolites on microsporidian Nosema ceranae and on its host Apis mellifera bacteriocin surfactin animal experiment animal model animal parasitosis antifungal activity article Bacillus bacterial strain bacterium isolation concentration response controlled study drug isolation Enterococcus fungus spore honey honeybee infection prevention inoculation metabolite Microsporidia midgut nonhuman Nosema Nosema cerenae nosemosis nucleotide sequence parasite development priority journal risk reduction toxicity testing Animals Antifungal Agents Bacillus Bees DNA, Fungal Enterococcus Gastrointestinal Tract Lipopeptides Molecular Sequence Data Nosema Peptides, Cyclic Sequence Analysis, DNA Apis mellifera Apoidea Bacillus (bacterium) Bacteria (microorganisms) Enterococcus Nosema Nosema ceranae Nosemosis, a disease caused by a microsporidian infection, is one of the most frequently observed parasitic pathologies affecting adult honeybees. Presently, Nosema ceranae seems to be the main microsporidian infection in Apis mellifera. The antibiotic fumagillin is the only compound available to treat Nosema diseases; however, it is no longer licensed in most EU member states; therefore, the need to identify new molecules/substances prevails. The intent of this paper is to test bacterial metabolites by Bacillus and Enterococcus strains, isolated from bee midgut and honey. The toxicity on bees and the antiparasitic activity on N. ceranae were assessed under laboratory conditions. Results did not yield toxicity for the administered surfactin or bacteriocin concentrations. Spores exposed to direct contact with a particular surfactin revealed a significant infectivity reduction when inoculated on bees. This surfactin, administered ad libitum from the individuals' emergence, led to a significant reduction in parasitosis development when bees were infected with untreated spores 7 days postemergence. Based on the results obtained, one of the surfactins is herein postulated as a molecule capable of reducing N. ceranae development, acting either by direct exposure to purified spores or incorporated into the digestive tract of the bee. © 2010 Springer-Verlag. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09320113_v107_n2_p381_Porrini http://hdl.handle.net/20.500.12110/paper_09320113_v107_n2_p381_Porrini |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
bacteriocin surfactin animal experiment animal model animal parasitosis antifungal activity article Bacillus bacterial strain bacterium isolation concentration response controlled study drug isolation Enterococcus fungus spore honey honeybee infection prevention inoculation metabolite Microsporidia midgut nonhuman Nosema Nosema cerenae nosemosis nucleotide sequence parasite development priority journal risk reduction toxicity testing Animals Antifungal Agents Bacillus Bees DNA, Fungal Enterococcus Gastrointestinal Tract Lipopeptides Molecular Sequence Data Nosema Peptides, Cyclic Sequence Analysis, DNA Apis mellifera Apoidea Bacillus (bacterium) Bacteria (microorganisms) Enterococcus Nosema Nosema ceranae |
| spellingShingle |
bacteriocin surfactin animal experiment animal model animal parasitosis antifungal activity article Bacillus bacterial strain bacterium isolation concentration response controlled study drug isolation Enterococcus fungus spore honey honeybee infection prevention inoculation metabolite Microsporidia midgut nonhuman Nosema Nosema cerenae nosemosis nucleotide sequence parasite development priority journal risk reduction toxicity testing Animals Antifungal Agents Bacillus Bees DNA, Fungal Enterococcus Gastrointestinal Tract Lipopeptides Molecular Sequence Data Nosema Peptides, Cyclic Sequence Analysis, DNA Apis mellifera Apoidea Bacillus (bacterium) Bacteria (microorganisms) Enterococcus Nosema Nosema ceranae Effect of bacterial metabolites on microsporidian Nosema ceranae and on its host Apis mellifera |
| topic_facet |
bacteriocin surfactin animal experiment animal model animal parasitosis antifungal activity article Bacillus bacterial strain bacterium isolation concentration response controlled study drug isolation Enterococcus fungus spore honey honeybee infection prevention inoculation metabolite Microsporidia midgut nonhuman Nosema Nosema cerenae nosemosis nucleotide sequence parasite development priority journal risk reduction toxicity testing Animals Antifungal Agents Bacillus Bees DNA, Fungal Enterococcus Gastrointestinal Tract Lipopeptides Molecular Sequence Data Nosema Peptides, Cyclic Sequence Analysis, DNA Apis mellifera Apoidea Bacillus (bacterium) Bacteria (microorganisms) Enterococcus Nosema Nosema ceranae |
| description |
Nosemosis, a disease caused by a microsporidian infection, is one of the most frequently observed parasitic pathologies affecting adult honeybees. Presently, Nosema ceranae seems to be the main microsporidian infection in Apis mellifera. The antibiotic fumagillin is the only compound available to treat Nosema diseases; however, it is no longer licensed in most EU member states; therefore, the need to identify new molecules/substances prevails. The intent of this paper is to test bacterial metabolites by Bacillus and Enterococcus strains, isolated from bee midgut and honey. The toxicity on bees and the antiparasitic activity on N. ceranae were assessed under laboratory conditions. Results did not yield toxicity for the administered surfactin or bacteriocin concentrations. Spores exposed to direct contact with a particular surfactin revealed a significant infectivity reduction when inoculated on bees. This surfactin, administered ad libitum from the individuals' emergence, led to a significant reduction in parasitosis development when bees were infected with untreated spores 7 days postemergence. Based on the results obtained, one of the surfactins is herein postulated as a molecule capable of reducing N. ceranae development, acting either by direct exposure to purified spores or incorporated into the digestive tract of the bee. © 2010 Springer-Verlag. |
| title |
Effect of bacterial metabolites on microsporidian Nosema ceranae and on its host Apis mellifera |
| title_short |
Effect of bacterial metabolites on microsporidian Nosema ceranae and on its host Apis mellifera |
| title_full |
Effect of bacterial metabolites on microsporidian Nosema ceranae and on its host Apis mellifera |
| title_fullStr |
Effect of bacterial metabolites on microsporidian Nosema ceranae and on its host Apis mellifera |
| title_full_unstemmed |
Effect of bacterial metabolites on microsporidian Nosema ceranae and on its host Apis mellifera |
| title_sort |
effect of bacterial metabolites on microsporidian nosema ceranae and on its host apis mellifera |
| publishDate |
2010 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09320113_v107_n2_p381_Porrini http://hdl.handle.net/20.500.12110/paper_09320113_v107_n2_p381_Porrini |
| _version_ |
1768544827160395776 |