Hydrolytic activities of Fusarium solani and Fusarium solani f. sp. eumartii associated with the infection process of potato tubers
The development of dry rot caused by Fusarium solani f. sp. eumartii was evaluated in susceptible (Huinkul) and resistant (Spunta) potato cultivars. Fungal proteolytic and polygalacturanase activities were measured at different days postinoculation either with the pathogenic F. solani f. sp. eumarti...
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2004
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09311785_v152_n6_p337_Olivieri http://hdl.handle.net/20.500.12110/paper_09311785_v152_n6_p337_Olivieri |
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paper:paper_09311785_v152_n6_p337_Olivieri2023-06-08T15:52:54Z Hydrolytic activities of Fusarium solani and Fusarium solani f. sp. eumartii associated with the infection process of potato tubers Cell wall degrading enzymes Fungal proteases Fusarium solani Solanum tuberosum disease resistance enzyme activity infectious disease inoculation tuber Fungi Fusarium Fusarium solani Fusarium sp. Solanum Solanum tuberosum The development of dry rot caused by Fusarium solani f. sp. eumartii was evaluated in susceptible (Huinkul) and resistant (Spunta) potato cultivars. Fungal proteolytic and polygalacturanase activities were measured at different days postinoculation either with the pathogenic F. solani f. sp. eumartii, isolate 3122 or with the non-pathogenic F. solani, isolate 1042. After inoculation with the pathogenic fungus, proteolytic and polygalaturonase activities were higher in the susceptible than in the resistant cultivar. In addition, we found a correlation between the levels of proteolytic activity detected in the intercellular washing fluids with the size of the lesion area caused by F. solani f. sp. eumartii in Huinkul tubers. The action of the proteolytic activity over cell wall proteins of both potato cultivars was assayed. An extracellular potato protein with homology to proteinase inhibitors of the Kunitz family was identified as a substrate of the proteolytic activity in the susceptible cultivar. A microscopic study revealed differences between the potato genotypes in the rate of response to infection by F. solani f. sp. eumartii. In addition, the cell wall alteration caused by F. solani f. sp. eumartii in cortical cells of susceptible tubers was evaluated. The data with respect to the correlation between the course of cyto- and biochemical events of the two host-pathogen interactions were discussed. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09311785_v152_n6_p337_Olivieri http://hdl.handle.net/20.500.12110/paper_09311785_v152_n6_p337_Olivieri |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Cell wall degrading enzymes Fungal proteases Fusarium solani Solanum tuberosum disease resistance enzyme activity infectious disease inoculation tuber Fungi Fusarium Fusarium solani Fusarium sp. Solanum Solanum tuberosum |
spellingShingle |
Cell wall degrading enzymes Fungal proteases Fusarium solani Solanum tuberosum disease resistance enzyme activity infectious disease inoculation tuber Fungi Fusarium Fusarium solani Fusarium sp. Solanum Solanum tuberosum Hydrolytic activities of Fusarium solani and Fusarium solani f. sp. eumartii associated with the infection process of potato tubers |
topic_facet |
Cell wall degrading enzymes Fungal proteases Fusarium solani Solanum tuberosum disease resistance enzyme activity infectious disease inoculation tuber Fungi Fusarium Fusarium solani Fusarium sp. Solanum Solanum tuberosum |
description |
The development of dry rot caused by Fusarium solani f. sp. eumartii was evaluated in susceptible (Huinkul) and resistant (Spunta) potato cultivars. Fungal proteolytic and polygalacturanase activities were measured at different days postinoculation either with the pathogenic F. solani f. sp. eumartii, isolate 3122 or with the non-pathogenic F. solani, isolate 1042. After inoculation with the pathogenic fungus, proteolytic and polygalaturonase activities were higher in the susceptible than in the resistant cultivar. In addition, we found a correlation between the levels of proteolytic activity detected in the intercellular washing fluids with the size of the lesion area caused by F. solani f. sp. eumartii in Huinkul tubers. The action of the proteolytic activity over cell wall proteins of both potato cultivars was assayed. An extracellular potato protein with homology to proteinase inhibitors of the Kunitz family was identified as a substrate of the proteolytic activity in the susceptible cultivar. A microscopic study revealed differences between the potato genotypes in the rate of response to infection by F. solani f. sp. eumartii. In addition, the cell wall alteration caused by F. solani f. sp. eumartii in cortical cells of susceptible tubers was evaluated. The data with respect to the correlation between the course of cyto- and biochemical events of the two host-pathogen interactions were discussed. |
title |
Hydrolytic activities of Fusarium solani and Fusarium solani f. sp. eumartii associated with the infection process of potato tubers |
title_short |
Hydrolytic activities of Fusarium solani and Fusarium solani f. sp. eumartii associated with the infection process of potato tubers |
title_full |
Hydrolytic activities of Fusarium solani and Fusarium solani f. sp. eumartii associated with the infection process of potato tubers |
title_fullStr |
Hydrolytic activities of Fusarium solani and Fusarium solani f. sp. eumartii associated with the infection process of potato tubers |
title_full_unstemmed |
Hydrolytic activities of Fusarium solani and Fusarium solani f. sp. eumartii associated with the infection process of potato tubers |
title_sort |
hydrolytic activities of fusarium solani and fusarium solani f. sp. eumartii associated with the infection process of potato tubers |
publishDate |
2004 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09311785_v152_n6_p337_Olivieri http://hdl.handle.net/20.500.12110/paper_09311785_v152_n6_p337_Olivieri |
_version_ |
1768545885606641664 |