Immunization with recombinant Trypanosoma cruzi ribosomal P2β protein induces changes in the electrocardiogram of immunized mice
Molecular expression cloning techniques revealed that patients with severe chronic Chagas heart disease showed a strong humoral response against the cloned C-terminal portion of the Trypanosoma cruzi ribosomal P2β protein, previously named JL5. The main linear epitope of this polypeptide was mapped...
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1997
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09288244_v18_n1_p75_LopezBergami http://hdl.handle.net/20.500.12110/paper_09288244_v18_n1_p75_LopezBergami |
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paper:paper_09288244_v18_n1_p75_LopezBergami2023-06-08T15:52:20Z Immunization with recombinant Trypanosoma cruzi ribosomal P2β protein induces changes in the electrocardiogram of immunized mice Chagas disease Electrocardiogram Functional assay Heart Immunization Ribosomal P protein Trypanosoma cruzi animal model article chagas disease electrocardiogram enzyme linked immunosorbent assay human humoral immunity immunization mouse nonhuman priority journal trypanosoma cruzi Animals Antibodies, Protozoan Autoantibodies Carrier Proteins Electrocardiography Heart Humans Immunization Mice Mice, Inbred BALB C Myocardium Protozoan Proteins Recombinant Fusion Proteins Ribosomal Proteins Trypanosoma cruzi Molecular expression cloning techniques revealed that patients with severe chronic Chagas heart disease showed a strong humoral response against the cloned C-terminal portion of the Trypanosoma cruzi ribosomal P2β protein, previously named JL5. The main linear epitope of this polypeptide was mapped to the 13 C-terminal amino acid sequence EEEDDDMGFGLFD (named R13), which is almost identical to the mammalian ribosomal P consensus sequence EESDDDMGFGLFD (named H13). Enzyme-linked immunosorbent assay measurements demonstrated that sera from patients with chronic Chagas heart disease presented a very specific anti-P humoral response with high anti- R13, but low H13 antibody levels. We attempted to develop an animal model that would reproduce, at least partially, two features of the human infection: (1) the serological pattern of the anti-P response, and (2) specific cardiac symptoms. To this effect, mice were immunized with T. cruzi P2β recombinant protein. Immunization reproduced the typical anti-P antibody profile defined for chronic infections, but did not induce cardiac inflammatory lesions. However, it altered significantly the electrocardiograms of immunized mice. It is suggested that this assay represents a functional test for assessing the biological activity of antibodies against T. cruzi ribosomal P protein on cardiac muscle. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09288244_v18_n1_p75_LopezBergami http://hdl.handle.net/20.500.12110/paper_09288244_v18_n1_p75_LopezBergami |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chagas disease Electrocardiogram Functional assay Heart Immunization Ribosomal P protein Trypanosoma cruzi animal model article chagas disease electrocardiogram enzyme linked immunosorbent assay human humoral immunity immunization mouse nonhuman priority journal trypanosoma cruzi Animals Antibodies, Protozoan Autoantibodies Carrier Proteins Electrocardiography Heart Humans Immunization Mice Mice, Inbred BALB C Myocardium Protozoan Proteins Recombinant Fusion Proteins Ribosomal Proteins Trypanosoma cruzi |
| spellingShingle |
Chagas disease Electrocardiogram Functional assay Heart Immunization Ribosomal P protein Trypanosoma cruzi animal model article chagas disease electrocardiogram enzyme linked immunosorbent assay human humoral immunity immunization mouse nonhuman priority journal trypanosoma cruzi Animals Antibodies, Protozoan Autoantibodies Carrier Proteins Electrocardiography Heart Humans Immunization Mice Mice, Inbred BALB C Myocardium Protozoan Proteins Recombinant Fusion Proteins Ribosomal Proteins Trypanosoma cruzi Immunization with recombinant Trypanosoma cruzi ribosomal P2β protein induces changes in the electrocardiogram of immunized mice |
| topic_facet |
Chagas disease Electrocardiogram Functional assay Heart Immunization Ribosomal P protein Trypanosoma cruzi animal model article chagas disease electrocardiogram enzyme linked immunosorbent assay human humoral immunity immunization mouse nonhuman priority journal trypanosoma cruzi Animals Antibodies, Protozoan Autoantibodies Carrier Proteins Electrocardiography Heart Humans Immunization Mice Mice, Inbred BALB C Myocardium Protozoan Proteins Recombinant Fusion Proteins Ribosomal Proteins Trypanosoma cruzi |
| description |
Molecular expression cloning techniques revealed that patients with severe chronic Chagas heart disease showed a strong humoral response against the cloned C-terminal portion of the Trypanosoma cruzi ribosomal P2β protein, previously named JL5. The main linear epitope of this polypeptide was mapped to the 13 C-terminal amino acid sequence EEEDDDMGFGLFD (named R13), which is almost identical to the mammalian ribosomal P consensus sequence EESDDDMGFGLFD (named H13). Enzyme-linked immunosorbent assay measurements demonstrated that sera from patients with chronic Chagas heart disease presented a very specific anti-P humoral response with high anti- R13, but low H13 antibody levels. We attempted to develop an animal model that would reproduce, at least partially, two features of the human infection: (1) the serological pattern of the anti-P response, and (2) specific cardiac symptoms. To this effect, mice were immunized with T. cruzi P2β recombinant protein. Immunization reproduced the typical anti-P antibody profile defined for chronic infections, but did not induce cardiac inflammatory lesions. However, it altered significantly the electrocardiograms of immunized mice. It is suggested that this assay represents a functional test for assessing the biological activity of antibodies against T. cruzi ribosomal P protein on cardiac muscle. |
| title |
Immunization with recombinant Trypanosoma cruzi ribosomal P2β protein induces changes in the electrocardiogram of immunized mice |
| title_short |
Immunization with recombinant Trypanosoma cruzi ribosomal P2β protein induces changes in the electrocardiogram of immunized mice |
| title_full |
Immunization with recombinant Trypanosoma cruzi ribosomal P2β protein induces changes in the electrocardiogram of immunized mice |
| title_fullStr |
Immunization with recombinant Trypanosoma cruzi ribosomal P2β protein induces changes in the electrocardiogram of immunized mice |
| title_full_unstemmed |
Immunization with recombinant Trypanosoma cruzi ribosomal P2β protein induces changes in the electrocardiogram of immunized mice |
| title_sort |
immunization with recombinant trypanosoma cruzi ribosomal p2β protein induces changes in the electrocardiogram of immunized mice |
| publishDate |
1997 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09288244_v18_n1_p75_LopezBergami http://hdl.handle.net/20.500.12110/paper_09288244_v18_n1_p75_LopezBergami |
| _version_ |
1768545655138025472 |