Proposed molecular model for electrostatic interactions between insulin and chitosan. Nano-complexation and activity in cultured cells
The objective of this contribution was to propose a model that would explain the nanocomplexes formation between Human Recombinant Insulin (I) and a polydisperse Chitosan (CS). Such an objective implied exploring I and CS concentration conditions that allowed the formation of complexes with defined...
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2018
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09277757_v537_n_p425_Silva http://hdl.handle.net/20.500.12110/paper_09277757_v537_n_p425_Silva |
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paper:paper_09277757_v537_n_p425_Silva2023-06-08T15:52:02Z Proposed molecular model for electrostatic interactions between insulin and chitosan. Nano-complexation and activity in cultured cells Chitosan Insulin Macromolecular assembly Nano-complexes Bioactivity Cell culture Chitin Chitosan Dynamic light scattering Electrostatics Light scattering Nanostructures Zeta potential Absorbance measurements Complexation process Controlled release Different stages Flow behaviors Nano-complexes Solution dynamics Surface proteins Insulin biopolymer chitosan membrane protein nanoshell protein aggregate recombinant human insulin 3T3-L1 cell line Article biological activity cell culture colloid complex formation concentration (parameters) controlled release formulation flow kinetics kinetic parameters macromolecule molecular model nanotechnology particle size pH photon correlation spectroscopy priority journal static electricity time factor viscosity zeta potential The objective of this contribution was to propose a model that would explain the nanocomplexes formation between Human Recombinant Insulin (I) and a polydisperse Chitosan (CS). Such an objective implied exploring I and CS concentration conditions that allowed the formation of complexes with defined and reproducible submicronic dimensions. I-CS complexes were obtained by mixing I and CS solutions at pH 2 and then increasing the pH up to 6 promoting electrostatic interactions between them. Colloidal stages of I and I-CS nano-complexes formation were characterized by dynamic light scattering (DLS), ζ-potential, solutions flow behavior and absorbance measurements. 1·10−2%, w/w, of CS allowed covering completely the surface protein aggregates constituting core–shell nano-structures of 200 nm, with a ζ-potential of 17,5 mV. Solution dynamic viscosity results kept relation with different stages of nano-complexation process. Biological activity of I-CS complexes was studied in 3T3-L1 cultured fibroblast showing a delayed and sustained activity as compared to free insulin. I-CS nano-complexes could be an alternative for developing a new generation of drugs allowing I protection from the hostile conditions of the body and increasing its absorption. These findings have basic and practical impacts as they could be exploited to exert the controlled release of I in therapeutic formulations by using the I-CS nano-complexes. © 2017 Elsevier B.V. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09277757_v537_n_p425_Silva http://hdl.handle.net/20.500.12110/paper_09277757_v537_n_p425_Silva |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Chitosan Insulin Macromolecular assembly Nano-complexes Bioactivity Cell culture Chitin Chitosan Dynamic light scattering Electrostatics Light scattering Nanostructures Zeta potential Absorbance measurements Complexation process Controlled release Different stages Flow behaviors Nano-complexes Solution dynamics Surface proteins Insulin biopolymer chitosan membrane protein nanoshell protein aggregate recombinant human insulin 3T3-L1 cell line Article biological activity cell culture colloid complex formation concentration (parameters) controlled release formulation flow kinetics kinetic parameters macromolecule molecular model nanotechnology particle size pH photon correlation spectroscopy priority journal static electricity time factor viscosity zeta potential |
| spellingShingle |
Chitosan Insulin Macromolecular assembly Nano-complexes Bioactivity Cell culture Chitin Chitosan Dynamic light scattering Electrostatics Light scattering Nanostructures Zeta potential Absorbance measurements Complexation process Controlled release Different stages Flow behaviors Nano-complexes Solution dynamics Surface proteins Insulin biopolymer chitosan membrane protein nanoshell protein aggregate recombinant human insulin 3T3-L1 cell line Article biological activity cell culture colloid complex formation concentration (parameters) controlled release formulation flow kinetics kinetic parameters macromolecule molecular model nanotechnology particle size pH photon correlation spectroscopy priority journal static electricity time factor viscosity zeta potential Proposed molecular model for electrostatic interactions between insulin and chitosan. Nano-complexation and activity in cultured cells |
| topic_facet |
Chitosan Insulin Macromolecular assembly Nano-complexes Bioactivity Cell culture Chitin Chitosan Dynamic light scattering Electrostatics Light scattering Nanostructures Zeta potential Absorbance measurements Complexation process Controlled release Different stages Flow behaviors Nano-complexes Solution dynamics Surface proteins Insulin biopolymer chitosan membrane protein nanoshell protein aggregate recombinant human insulin 3T3-L1 cell line Article biological activity cell culture colloid complex formation concentration (parameters) controlled release formulation flow kinetics kinetic parameters macromolecule molecular model nanotechnology particle size pH photon correlation spectroscopy priority journal static electricity time factor viscosity zeta potential |
| description |
The objective of this contribution was to propose a model that would explain the nanocomplexes formation between Human Recombinant Insulin (I) and a polydisperse Chitosan (CS). Such an objective implied exploring I and CS concentration conditions that allowed the formation of complexes with defined and reproducible submicronic dimensions. I-CS complexes were obtained by mixing I and CS solutions at pH 2 and then increasing the pH up to 6 promoting electrostatic interactions between them. Colloidal stages of I and I-CS nano-complexes formation were characterized by dynamic light scattering (DLS), ζ-potential, solutions flow behavior and absorbance measurements. 1·10−2%, w/w, of CS allowed covering completely the surface protein aggregates constituting core–shell nano-structures of 200 nm, with a ζ-potential of 17,5 mV. Solution dynamic viscosity results kept relation with different stages of nano-complexation process. Biological activity of I-CS complexes was studied in 3T3-L1 cultured fibroblast showing a delayed and sustained activity as compared to free insulin. I-CS nano-complexes could be an alternative for developing a new generation of drugs allowing I protection from the hostile conditions of the body and increasing its absorption. These findings have basic and practical impacts as they could be exploited to exert the controlled release of I in therapeutic formulations by using the I-CS nano-complexes. © 2017 Elsevier B.V. |
| title |
Proposed molecular model for electrostatic interactions between insulin and chitosan. Nano-complexation and activity in cultured cells |
| title_short |
Proposed molecular model for electrostatic interactions between insulin and chitosan. Nano-complexation and activity in cultured cells |
| title_full |
Proposed molecular model for electrostatic interactions between insulin and chitosan. Nano-complexation and activity in cultured cells |
| title_fullStr |
Proposed molecular model for electrostatic interactions between insulin and chitosan. Nano-complexation and activity in cultured cells |
| title_full_unstemmed |
Proposed molecular model for electrostatic interactions between insulin and chitosan. Nano-complexation and activity in cultured cells |
| title_sort |
proposed molecular model for electrostatic interactions between insulin and chitosan. nano-complexation and activity in cultured cells |
| publishDate |
2018 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09277757_v537_n_p425_Silva http://hdl.handle.net/20.500.12110/paper_09277757_v537_n_p425_Silva |
| _version_ |
1768544142281932800 |