Surface enhanced Raman spectroscopy and cultural heritage biodeterioration: Fungi identification in earthen architecture from Paraíba Valley (São Paulo, Brazil)

In this work, Surface Enhanced Raman Spectroscopy (SERS) was employed in the taxonomic identification of fungi found in biofilms formed on earthen architecture walls (adobe, wattle and daub, and rammed earth) of historical buildings in the region known as Paraíba Valley (or São Paulo Historical Vall...

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Detalles Bibliográficos
Publicado: 2018
Materias:
Biodeterioration
Earthen architecture
Fungi
Paraíba Valley
Raman
SERS
Architecture
Biodegradation
Biofilms
Deterioration
Diagnosis
Gold nanoparticles
Landforms
Light transmission
Molecular biology
Raman spectroscopy
Substrates
Walls (structural partitions)
Earthen architectures
Potato dextrose agars
SERS-active substrates
Surface enhanced Raman spectroscopy
Taxonomic identifications
Architectural acoustics
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09242031_v97_n_p129_Fazio
http://hdl.handle.net/20.500.12110/paper_09242031_v97_n_p129_Fazio
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_09242031_v97_n_p129_Fazio
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spelling paper:paper_09242031_v97_n_p129_Fazio2023-06-08T15:51:03Z Surface enhanced Raman spectroscopy and cultural heritage biodeterioration: Fungi identification in earthen architecture from Paraíba Valley (São Paulo, Brazil) Biodeterioration Earthen architecture Fungi Paraíba Valley Raman SERS Architecture Biodegradation Biofilms Deterioration Diagnosis Fungi Gold nanoparticles Landforms Light transmission Molecular biology Raman spectroscopy Substrates Walls (structural partitions) Biodeterioration Earthen architectures Potato dextrose agars Raman SERS SERS-active substrates Surface enhanced Raman spectroscopy Taxonomic identifications Architectural acoustics In this work, Surface Enhanced Raman Spectroscopy (SERS) was employed in the taxonomic identification of fungi found in biofilms formed on earthen architecture walls (adobe, wattle and daub, and rammed earth) of historical buildings in the region known as Paraíba Valley (or São Paulo Historical Valley), which are representative of the first phase of the Brazilian coffee cycle (1820–1880). Very few studies are reported in the literature where SERS-based techniques are used in fungi identification, most of them focused on clinical diagnosis. In the present investigation, pure colonies isolated from biofilms on earthen walls previously identified by classic taxonomy and molecular biology were selected. The genera were Trichoderma, Cladosporium, Aspergillus, Neurospora, Fusarium and Penicillium. The fungi were cultured on solid potato dextrose agar, extracted with ethyl acetate and the extracts were applied on dried Au nanoparticles. The SERS spectra exhibited bands in the 600–1800 cm−1 region which are characteristic of each genus, except Penicillium, as revealed by PCA statistical analysis. This work reports the use of a facile to prepare SERS-active substrate in the identification of microbial communities on earthen architecture walls and is the first step of an investigation aiming at the fast identification of fungi species from biofilms formed on earthen architecture buildings without the need of isolating the pure cultures. © 2018 Elsevier B.V. 2018 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09242031_v97_n_p129_Fazio http://hdl.handle.net/20.500.12110/paper_09242031_v97_n_p129_Fazio
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Biodeterioration
Earthen architecture
Fungi
Paraíba Valley
Raman
SERS
Architecture
Biodegradation
Biofilms
Deterioration
Diagnosis
Fungi
Gold nanoparticles
Landforms
Light transmission
Molecular biology
Raman spectroscopy
Substrates
Walls (structural partitions)
Biodeterioration
Earthen architectures
Potato dextrose agars
Raman
SERS
SERS-active substrates
Surface enhanced Raman spectroscopy
Taxonomic identifications
Architectural acoustics
spellingShingle Biodeterioration
Earthen architecture
Fungi
Paraíba Valley
Raman
SERS
Architecture
Biodegradation
Biofilms
Deterioration
Diagnosis
Fungi
Gold nanoparticles
Landforms
Light transmission
Molecular biology
Raman spectroscopy
Substrates
Walls (structural partitions)
Biodeterioration
Earthen architectures
Potato dextrose agars
Raman
SERS
SERS-active substrates
Surface enhanced Raman spectroscopy
Taxonomic identifications
Architectural acoustics
Surface enhanced Raman spectroscopy and cultural heritage biodeterioration: Fungi identification in earthen architecture from Paraíba Valley (São Paulo, Brazil)
topic_facet Biodeterioration
Earthen architecture
Fungi
Paraíba Valley
Raman
SERS
Architecture
Biodegradation
Biofilms
Deterioration
Diagnosis
Fungi
Gold nanoparticles
Landforms
Light transmission
Molecular biology
Raman spectroscopy
Substrates
Walls (structural partitions)
Biodeterioration
Earthen architectures
Potato dextrose agars
Raman
SERS
SERS-active substrates
Surface enhanced Raman spectroscopy
Taxonomic identifications
Architectural acoustics
description In this work, Surface Enhanced Raman Spectroscopy (SERS) was employed in the taxonomic identification of fungi found in biofilms formed on earthen architecture walls (adobe, wattle and daub, and rammed earth) of historical buildings in the region known as Paraíba Valley (or São Paulo Historical Valley), which are representative of the first phase of the Brazilian coffee cycle (1820–1880). Very few studies are reported in the literature where SERS-based techniques are used in fungi identification, most of them focused on clinical diagnosis. In the present investigation, pure colonies isolated from biofilms on earthen walls previously identified by classic taxonomy and molecular biology were selected. The genera were Trichoderma, Cladosporium, Aspergillus, Neurospora, Fusarium and Penicillium. The fungi were cultured on solid potato dextrose agar, extracted with ethyl acetate and the extracts were applied on dried Au nanoparticles. The SERS spectra exhibited bands in the 600–1800 cm−1 region which are characteristic of each genus, except Penicillium, as revealed by PCA statistical analysis. This work reports the use of a facile to prepare SERS-active substrate in the identification of microbial communities on earthen architecture walls and is the first step of an investigation aiming at the fast identification of fungi species from biofilms formed on earthen architecture buildings without the need of isolating the pure cultures. © 2018 Elsevier B.V.
title Surface enhanced Raman spectroscopy and cultural heritage biodeterioration: Fungi identification in earthen architecture from Paraíba Valley (São Paulo, Brazil)
title_short Surface enhanced Raman spectroscopy and cultural heritage biodeterioration: Fungi identification in earthen architecture from Paraíba Valley (São Paulo, Brazil)
title_full Surface enhanced Raman spectroscopy and cultural heritage biodeterioration: Fungi identification in earthen architecture from Paraíba Valley (São Paulo, Brazil)
title_fullStr Surface enhanced Raman spectroscopy and cultural heritage biodeterioration: Fungi identification in earthen architecture from Paraíba Valley (São Paulo, Brazil)
title_full_unstemmed Surface enhanced Raman spectroscopy and cultural heritage biodeterioration: Fungi identification in earthen architecture from Paraíba Valley (São Paulo, Brazil)
title_sort surface enhanced raman spectroscopy and cultural heritage biodeterioration: fungi identification in earthen architecture from paraíba valley (são paulo, brazil)
publishDate 2018
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09242031_v97_n_p129_Fazio
http://hdl.handle.net/20.500.12110/paper_09242031_v97_n_p129_Fazio
_version_ 1768546682503430144

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