A comparison of Junin virus strains: Growth characteristics, cytopathogenicity and viral polypeptides
The growth characteristics, cytopathogenicity and viral polypeptides of the virulent strain XJ of Junin virus (JV), its attenuated derivative XJC13 and another naturally attenuated JV strain, IV4454, were comparatively studied. IV4454 and XJC13 viruses showed the highest and lowest cytopathology for...
Guardado en:
| Autores principales: | , , , , |
|---|---|
| Publicado: |
1990
|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09232516_v141_n5_p505_Candurra http://hdl.handle.net/20.500.12110/paper_09232516_v141_n5_p505_Candurra |
| Aporte de: |
| id |
paper:paper_09232516_v141_n5_p505_Candurra |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_09232516_v141_n5_p505_Candurra2023-06-08T15:50:59Z A comparison of Junin virus strains: Growth characteristics, cytopathogenicity and viral polypeptides Candurra, Nélida Alicia Scolaro, Luis Alberto Mersich, Susana E. Damonte, Elsa Beatriz Coto, Celia Esther Arenaviridae Attenuated virus Cytopathogenicity Junin virus Polypeptide Strains Virulence virus protein article cytotoxicity human cell junin virus nonhuman priority journal virogenesis virus attenuation virus pathogenesis virus strain virus virulence Animal Arenaviruses, New World Cell Line Comparative Study Cytopathogenic Effect, Viral Peptide Mapping Plaque Assay Proteins Support, Non-U.S. Gov't Vero Cells Viral Proteins Arenaviridae Junin virus The growth characteristics, cytopathogenicity and viral polypeptides of the virulent strain XJ of Junin virus (JV), its attenuated derivative XJC13 and another naturally attenuated JV strain, IV4454, were comparatively studied. IV4454 and XJC13 viruses showed the highest and lowest cytopathology for Vero cells, respectively, as measured by plaque morphology, cell viability and inhibition of host cell protein synthesis. The kinetics and electrophoretic patterns of viral polypeptides in infected cell extracts were very similar among the three strains, whereas differences were detected in the surface glycoprotein GP38 by peptide mapping after limited proteolysis. © 1990. Fil:Candurra, N.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Scolaro, L.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mersich, S.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Coto, C.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1990 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09232516_v141_n5_p505_Candurra http://hdl.handle.net/20.500.12110/paper_09232516_v141_n5_p505_Candurra |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Arenaviridae Attenuated virus Cytopathogenicity Junin virus Polypeptide Strains Virulence virus protein article cytotoxicity human cell junin virus nonhuman priority journal virogenesis virus attenuation virus pathogenesis virus strain virus virulence Animal Arenaviruses, New World Cell Line Comparative Study Cytopathogenic Effect, Viral Peptide Mapping Plaque Assay Proteins Support, Non-U.S. Gov't Vero Cells Viral Proteins Arenaviridae Junin virus |
| spellingShingle |
Arenaviridae Attenuated virus Cytopathogenicity Junin virus Polypeptide Strains Virulence virus protein article cytotoxicity human cell junin virus nonhuman priority journal virogenesis virus attenuation virus pathogenesis virus strain virus virulence Animal Arenaviruses, New World Cell Line Comparative Study Cytopathogenic Effect, Viral Peptide Mapping Plaque Assay Proteins Support, Non-U.S. Gov't Vero Cells Viral Proteins Arenaviridae Junin virus Candurra, Nélida Alicia Scolaro, Luis Alberto Mersich, Susana E. Damonte, Elsa Beatriz Coto, Celia Esther A comparison of Junin virus strains: Growth characteristics, cytopathogenicity and viral polypeptides |
| topic_facet |
Arenaviridae Attenuated virus Cytopathogenicity Junin virus Polypeptide Strains Virulence virus protein article cytotoxicity human cell junin virus nonhuman priority journal virogenesis virus attenuation virus pathogenesis virus strain virus virulence Animal Arenaviruses, New World Cell Line Comparative Study Cytopathogenic Effect, Viral Peptide Mapping Plaque Assay Proteins Support, Non-U.S. Gov't Vero Cells Viral Proteins Arenaviridae Junin virus |
| description |
The growth characteristics, cytopathogenicity and viral polypeptides of the virulent strain XJ of Junin virus (JV), its attenuated derivative XJC13 and another naturally attenuated JV strain, IV4454, were comparatively studied. IV4454 and XJC13 viruses showed the highest and lowest cytopathology for Vero cells, respectively, as measured by plaque morphology, cell viability and inhibition of host cell protein synthesis. The kinetics and electrophoretic patterns of viral polypeptides in infected cell extracts were very similar among the three strains, whereas differences were detected in the surface glycoprotein GP38 by peptide mapping after limited proteolysis. © 1990. |
| author |
Candurra, Nélida Alicia Scolaro, Luis Alberto Mersich, Susana E. Damonte, Elsa Beatriz Coto, Celia Esther |
| author_facet |
Candurra, Nélida Alicia Scolaro, Luis Alberto Mersich, Susana E. Damonte, Elsa Beatriz Coto, Celia Esther |
| author_sort |
Candurra, Nélida Alicia |
| title |
A comparison of Junin virus strains: Growth characteristics, cytopathogenicity and viral polypeptides |
| title_short |
A comparison of Junin virus strains: Growth characteristics, cytopathogenicity and viral polypeptides |
| title_full |
A comparison of Junin virus strains: Growth characteristics, cytopathogenicity and viral polypeptides |
| title_fullStr |
A comparison of Junin virus strains: Growth characteristics, cytopathogenicity and viral polypeptides |
| title_full_unstemmed |
A comparison of Junin virus strains: Growth characteristics, cytopathogenicity and viral polypeptides |
| title_sort |
comparison of junin virus strains: growth characteristics, cytopathogenicity and viral polypeptides |
| publishDate |
1990 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09232516_v141_n5_p505_Candurra http://hdl.handle.net/20.500.12110/paper_09232516_v141_n5_p505_Candurra |
| work_keys_str_mv |
AT candurranelidaalicia acomparisonofjuninvirusstrainsgrowthcharacteristicscytopathogenicityandviralpolypeptides AT scolaroluisalberto acomparisonofjuninvirusstrainsgrowthcharacteristicscytopathogenicityandviralpolypeptides AT mersichsusanae acomparisonofjuninvirusstrainsgrowthcharacteristicscytopathogenicityandviralpolypeptides AT damonteelsabeatriz acomparisonofjuninvirusstrainsgrowthcharacteristicscytopathogenicityandviralpolypeptides AT cotoceliaesther acomparisonofjuninvirusstrainsgrowthcharacteristicscytopathogenicityandviralpolypeptides AT candurranelidaalicia comparisonofjuninvirusstrainsgrowthcharacteristicscytopathogenicityandviralpolypeptides AT scolaroluisalberto comparisonofjuninvirusstrainsgrowthcharacteristicscytopathogenicityandviralpolypeptides AT mersichsusanae comparisonofjuninvirusstrainsgrowthcharacteristicscytopathogenicityandviralpolypeptides AT damonteelsabeatriz comparisonofjuninvirusstrainsgrowthcharacteristicscytopathogenicityandviralpolypeptides AT cotoceliaesther comparisonofjuninvirusstrainsgrowthcharacteristicscytopathogenicityandviralpolypeptides |
| _version_ |
1768546682322026496 |