Long-term antibodies after an oral immunization with cholera toxin are synthesized in the bone marrow and may play a role in the regulation of memory B-cell maintenance at systemic and mucosal sites
To study the importance of the bone marrow in the long-term antibody response, IgG and IgA antitoxin antibody-forming cells were evaluated by ELISPOT in Peyer's patches, mesenteric lymph nodes, spleen, lamina propria of the small intestine and bone marrow at several times after oral immunizatio...
Guardado en:
Autores principales: | , , |
---|---|
Publicado: |
1998
|
Materias: | |
Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09232494_v149_n2_p107_Benedetti http://hdl.handle.net/20.500.12110/paper_09232494_v149_n2_p107_Benedetti |
Aporte de: |
id |
paper:paper_09232494_v149_n2_p107_Benedetti |
---|---|
record_format |
dspace |
spelling |
paper:paper_09232494_v149_n2_p107_Benedetti2023-06-08T15:50:57Z Long-term antibodies after an oral immunization with cholera toxin are synthesized in the bone marrow and may play a role in the regulation of memory B-cell maintenance at systemic and mucosal sites Benedetti, Rubén Mariano Lev, Paola Roxana Massouh, Ernesto Jorge Anamnestic response B lymphocyte Bone marrow Cholera toxin Follicular dendritic cells IgA IgG Immunogenesis Long-term antibody response Mucosal immunization Rat antitoxin cholera toxin immunoglobulin A immunoglobulin G adoptive transfer animal cell animal experiment animal tissue antibody response article B lymphocyte bone marrow controlled study female immunoglobulin production memory cell mesentery lymph node nonhuman priority journal rat spleen T lymphocyte To study the importance of the bone marrow in the long-term antibody response, IgG and IgA antitoxin antibody-forming cells were evaluated by ELISPOT in Peyer's patches, mesenteric lymph nodes, spleen, lamina propria of the small intestine and bone marrow at several times after oral immunization with cholera toxin. The mesenteric lymph node was the site having the major frequency of IgG antitoxin during the first two weeks after priming, whereas lamina propria was the site with e major number of IgA antitoxin antibody- forming cells. However, from 3 weeks until 10 months after priming, bone marrow became the site with the major frequency of IgG, and especially IgA antitoxin antibody-forming cells (without taking into account the lamina propria). This result indicates that bone marrow was responsible for the long-term antibody response and raises questions concerning the mechanisms involved in the maintenance of antibody production. The importance of bone marrow as a site of antibody production was great when we analysed results as the true contribution of the total number of antitoxin antibody-forming cells, taking into account the number of cells recovered from each organ. When we analysed the anatomical location of memory B and T cells by adoptive transference, we found that cells from mesenteric lymph nodes and spleen were able to transfer a strong antibody response to naive syngeneic recipients, whereas bone marrow cells transferred a weak antibody response. Fil:Benedetti, R. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lev, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Massouh, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09232494_v149_n2_p107_Benedetti http://hdl.handle.net/20.500.12110/paper_09232494_v149_n2_p107_Benedetti |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Anamnestic response B lymphocyte Bone marrow Cholera toxin Follicular dendritic cells IgA IgG Immunogenesis Long-term antibody response Mucosal immunization Rat antitoxin cholera toxin immunoglobulin A immunoglobulin G adoptive transfer animal cell animal experiment animal tissue antibody response article B lymphocyte bone marrow controlled study female immunoglobulin production memory cell mesentery lymph node nonhuman priority journal rat spleen T lymphocyte |
spellingShingle |
Anamnestic response B lymphocyte Bone marrow Cholera toxin Follicular dendritic cells IgA IgG Immunogenesis Long-term antibody response Mucosal immunization Rat antitoxin cholera toxin immunoglobulin A immunoglobulin G adoptive transfer animal cell animal experiment animal tissue antibody response article B lymphocyte bone marrow controlled study female immunoglobulin production memory cell mesentery lymph node nonhuman priority journal rat spleen T lymphocyte Benedetti, Rubén Mariano Lev, Paola Roxana Massouh, Ernesto Jorge Long-term antibodies after an oral immunization with cholera toxin are synthesized in the bone marrow and may play a role in the regulation of memory B-cell maintenance at systemic and mucosal sites |
topic_facet |
Anamnestic response B lymphocyte Bone marrow Cholera toxin Follicular dendritic cells IgA IgG Immunogenesis Long-term antibody response Mucosal immunization Rat antitoxin cholera toxin immunoglobulin A immunoglobulin G adoptive transfer animal cell animal experiment animal tissue antibody response article B lymphocyte bone marrow controlled study female immunoglobulin production memory cell mesentery lymph node nonhuman priority journal rat spleen T lymphocyte |
description |
To study the importance of the bone marrow in the long-term antibody response, IgG and IgA antitoxin antibody-forming cells were evaluated by ELISPOT in Peyer's patches, mesenteric lymph nodes, spleen, lamina propria of the small intestine and bone marrow at several times after oral immunization with cholera toxin. The mesenteric lymph node was the site having the major frequency of IgG antitoxin during the first two weeks after priming, whereas lamina propria was the site with e major number of IgA antitoxin antibody- forming cells. However, from 3 weeks until 10 months after priming, bone marrow became the site with the major frequency of IgG, and especially IgA antitoxin antibody-forming cells (without taking into account the lamina propria). This result indicates that bone marrow was responsible for the long-term antibody response and raises questions concerning the mechanisms involved in the maintenance of antibody production. The importance of bone marrow as a site of antibody production was great when we analysed results as the true contribution of the total number of antitoxin antibody-forming cells, taking into account the number of cells recovered from each organ. When we analysed the anatomical location of memory B and T cells by adoptive transference, we found that cells from mesenteric lymph nodes and spleen were able to transfer a strong antibody response to naive syngeneic recipients, whereas bone marrow cells transferred a weak antibody response. |
author |
Benedetti, Rubén Mariano Lev, Paola Roxana Massouh, Ernesto Jorge |
author_facet |
Benedetti, Rubén Mariano Lev, Paola Roxana Massouh, Ernesto Jorge |
author_sort |
Benedetti, Rubén Mariano |
title |
Long-term antibodies after an oral immunization with cholera toxin are synthesized in the bone marrow and may play a role in the regulation of memory B-cell maintenance at systemic and mucosal sites |
title_short |
Long-term antibodies after an oral immunization with cholera toxin are synthesized in the bone marrow and may play a role in the regulation of memory B-cell maintenance at systemic and mucosal sites |
title_full |
Long-term antibodies after an oral immunization with cholera toxin are synthesized in the bone marrow and may play a role in the regulation of memory B-cell maintenance at systemic and mucosal sites |
title_fullStr |
Long-term antibodies after an oral immunization with cholera toxin are synthesized in the bone marrow and may play a role in the regulation of memory B-cell maintenance at systemic and mucosal sites |
title_full_unstemmed |
Long-term antibodies after an oral immunization with cholera toxin are synthesized in the bone marrow and may play a role in the regulation of memory B-cell maintenance at systemic and mucosal sites |
title_sort |
long-term antibodies after an oral immunization with cholera toxin are synthesized in the bone marrow and may play a role in the regulation of memory b-cell maintenance at systemic and mucosal sites |
publishDate |
1998 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09232494_v149_n2_p107_Benedetti http://hdl.handle.net/20.500.12110/paper_09232494_v149_n2_p107_Benedetti |
work_keys_str_mv |
AT benedettirubenmariano longtermantibodiesafteranoralimmunizationwithcholeratoxinaresynthesizedinthebonemarrowandmayplayaroleintheregulationofmemorybcellmaintenanceatsystemicandmucosalsites AT levpaolaroxana longtermantibodiesafteranoralimmunizationwithcholeratoxinaresynthesizedinthebonemarrowandmayplayaroleintheregulationofmemorybcellmaintenanceatsystemicandmucosalsites AT massouhernestojorge longtermantibodiesafteranoralimmunizationwithcholeratoxinaresynthesizedinthebonemarrowandmayplayaroleintheregulationofmemorybcellmaintenanceatsystemicandmucosalsites |
_version_ |
1768542650728710144 |