Growth hormone, luteinizing hormone, and follicle-stimulating hormone regulation by neuropeptide Y in both sexes of the cichlid fish, Cichlasoma dimerus

Neuropeptide Y (NPY) is considered the most potent orexigenic peptide, increasing before meal time and during fasting. In teleost, most studies on NPY action upon growth hormone (GH) and luteinizing hormone (LH) were conducted in females or group of animals without sex discrimination. The aim of thi...

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Detalles Bibliográficos
Autores principales: Di Yorio, María Paula, Vissio, Paula Gabriela
Publicado: 2015
Materias:
Cichlids
FSH
GH
LH
NPY
Animalia
Cichlasoma
Cichlidae
Teleostei
follitropin
growth hormone
luteinizing hormone
neuropeptide Y
animal
cichlid
drug effects
female
genetics
hypophysis
male
metabolism
Animals
Female
Follicle Stimulating Hormone
Growth Hormone
Luteinizing Hormone
Male
Neuropeptide Y
Pituitary Gland
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09201742_v41_n4_p843_DiYorio
http://hdl.handle.net/20.500.12110/paper_09201742_v41_n4_p843_DiYorio
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_09201742_v41_n4_p843_DiYorio
record_format dspace
spelling paper:paper_09201742_v41_n4_p843_DiYorio2023-06-08T15:50:04Z Growth hormone, luteinizing hormone, and follicle-stimulating hormone regulation by neuropeptide Y in both sexes of the cichlid fish, Cichlasoma dimerus Di Yorio, María Paula Vissio, Paula Gabriela Cichlids FSH GH LH NPY Animalia Cichlasoma Cichlidae Teleostei follitropin growth hormone luteinizing hormone neuropeptide Y animal cichlid drug effects female genetics hypophysis male metabolism Animals Cichlids Female Follicle Stimulating Hormone Growth Hormone Luteinizing Hormone Male Neuropeptide Y Pituitary Gland Neuropeptide Y (NPY) is considered the most potent orexigenic peptide, increasing before meal time and during fasting. In teleost, most studies on NPY action upon growth hormone (GH) and luteinizing hormone (LH) were conducted in females or group of animals without sex discrimination. The aim of this study was to evaluate whether NPY modulates the expression and release of GH and gonadotropins in both sexes of Cichlasoma dimerus. By double-label immunofluorescence, we first determined the association between NPY fibers and pituitary cells. In addition, we performed in vitro studies to evaluate the effect of NPY on GH and gonadotropins expression by real-time PCR, and release by Western blot, in males and females separately. Contacts between NPY fibers and GH and follicle-stimulating hormone (FSH)-producing cells were detected, indicating possible functional relationships. We observed an increase in GH release in the culture medium at 2 nM for males (p = 0.043) and 20 nM for females (p = 0.028). Pituitary FSH release was stimulated at 20 nM (p = 0.026) and 200 nM (p = 0.033) for males and females, respectively. Finally, NPY only increased β-LH mRNA expression at 20 nM in females (p = 0.028) and its release at 2 nM (p = 0.049) and 200 nM for males (p = 0.005) and 200 nM for females (p = 0.018). In conclusion, NPY acts as a GH-, LH- and FSH-releasing factor, in a dose- and sex-dependent way. © 2015, Springer Science+Business Media Dordrecht. Fil:Di Yorio, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vissio, P.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09201742_v41_n4_p843_DiYorio http://hdl.handle.net/20.500.12110/paper_09201742_v41_n4_p843_DiYorio
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Cichlids
FSH
GH
LH
NPY
Animalia
Cichlasoma
Cichlidae
Teleostei
follitropin
growth hormone
luteinizing hormone
neuropeptide Y
animal
cichlid
drug effects
female
genetics
hypophysis
male
metabolism
Animals
Cichlids
Female
Follicle Stimulating Hormone
Growth Hormone
Luteinizing Hormone
Male
Neuropeptide Y
Pituitary Gland
spellingShingle Cichlids
FSH
GH
LH
NPY
Animalia
Cichlasoma
Cichlidae
Teleostei
follitropin
growth hormone
luteinizing hormone
neuropeptide Y
animal
cichlid
drug effects
female
genetics
hypophysis
male
metabolism
Animals
Cichlids
Female
Follicle Stimulating Hormone
Growth Hormone
Luteinizing Hormone
Male
Neuropeptide Y
Pituitary Gland
Di Yorio, María Paula
Vissio, Paula Gabriela
Growth hormone, luteinizing hormone, and follicle-stimulating hormone regulation by neuropeptide Y in both sexes of the cichlid fish, Cichlasoma dimerus
topic_facet Cichlids
FSH
GH
LH
NPY
Animalia
Cichlasoma
Cichlidae
Teleostei
follitropin
growth hormone
luteinizing hormone
neuropeptide Y
animal
cichlid
drug effects
female
genetics
hypophysis
male
metabolism
Animals
Cichlids
Female
Follicle Stimulating Hormone
Growth Hormone
Luteinizing Hormone
Male
Neuropeptide Y
Pituitary Gland
description Neuropeptide Y (NPY) is considered the most potent orexigenic peptide, increasing before meal time and during fasting. In teleost, most studies on NPY action upon growth hormone (GH) and luteinizing hormone (LH) were conducted in females or group of animals without sex discrimination. The aim of this study was to evaluate whether NPY modulates the expression and release of GH and gonadotropins in both sexes of Cichlasoma dimerus. By double-label immunofluorescence, we first determined the association between NPY fibers and pituitary cells. In addition, we performed in vitro studies to evaluate the effect of NPY on GH and gonadotropins expression by real-time PCR, and release by Western blot, in males and females separately. Contacts between NPY fibers and GH and follicle-stimulating hormone (FSH)-producing cells were detected, indicating possible functional relationships. We observed an increase in GH release in the culture medium at 2 nM for males (p = 0.043) and 20 nM for females (p = 0.028). Pituitary FSH release was stimulated at 20 nM (p = 0.026) and 200 nM (p = 0.033) for males and females, respectively. Finally, NPY only increased β-LH mRNA expression at 20 nM in females (p = 0.028) and its release at 2 nM (p = 0.049) and 200 nM for males (p = 0.005) and 200 nM for females (p = 0.018). In conclusion, NPY acts as a GH-, LH- and FSH-releasing factor, in a dose- and sex-dependent way. © 2015, Springer Science+Business Media Dordrecht.
author Di Yorio, María Paula
Vissio, Paula Gabriela
author_facet Di Yorio, María Paula
Vissio, Paula Gabriela
author_sort Di Yorio, María Paula
title Growth hormone, luteinizing hormone, and follicle-stimulating hormone regulation by neuropeptide Y in both sexes of the cichlid fish, Cichlasoma dimerus
title_short Growth hormone, luteinizing hormone, and follicle-stimulating hormone regulation by neuropeptide Y in both sexes of the cichlid fish, Cichlasoma dimerus
title_full Growth hormone, luteinizing hormone, and follicle-stimulating hormone regulation by neuropeptide Y in both sexes of the cichlid fish, Cichlasoma dimerus
title_fullStr Growth hormone, luteinizing hormone, and follicle-stimulating hormone regulation by neuropeptide Y in both sexes of the cichlid fish, Cichlasoma dimerus
title_full_unstemmed Growth hormone, luteinizing hormone, and follicle-stimulating hormone regulation by neuropeptide Y in both sexes of the cichlid fish, Cichlasoma dimerus
title_sort growth hormone, luteinizing hormone, and follicle-stimulating hormone regulation by neuropeptide y in both sexes of the cichlid fish, cichlasoma dimerus
publishDate 2015
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09201742_v41_n4_p843_DiYorio
http://hdl.handle.net/20.500.12110/paper_09201742_v41_n4_p843_DiYorio
work_keys_str_mv AT diyoriomariapaula growthhormoneluteinizinghormoneandfolliclestimulatinghormoneregulationbyneuropeptideyinbothsexesofthecichlidfishcichlasomadimerus
AT vissiopaulagabriela growthhormoneluteinizinghormoneandfolliclestimulatinghormoneregulationbyneuropeptideyinbothsexesofthecichlidfishcichlasomadimerus
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