GH, IGF-I and GH receptors mRNA expression in response to growth impairment following a food deprivation period in individually housed cichlid fish Cichlasoma dimerus
Cichlasoma dimerus is a social cichlid fish capable of growing at high rates under laboratory conditions, but knowledge on somatic growth regulation is still unclear. Growth hormone (GH)/insulin-like growth factor I (IGF-I) axis is the key regulator of somatic growth in vertebrates. Two types of gro...
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09201742_v41_n1_p51_Delgadin http://hdl.handle.net/20.500.12110/paper_09201742_v41_n1_p51_Delgadin |
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paper:paper_09201742_v41_n1_p51_Delgadin2023-06-08T15:50:04Z GH, IGF-I and GH receptors mRNA expression in response to growth impairment following a food deprivation period in individually housed cichlid fish Cichlasoma dimerus Di Yorio, María Paula Vissio, Paula Gabriela Cichlid GHR1 GHR2 Growth hormone IGF-I growth hormone growth hormone receptor messenger RNA primer DNA somatomedin C analysis of variance animal body size cichlid DNA sequence food deprivation gene expression regulation genetics growth, development and aging liver metabolism molecular genetics nucleotide sequence physiology real time polymerase chain reaction skeletal muscle Analysis of Variance Animals Base Sequence Body Size Cichlids DNA Primers Food Deprivation Gene Expression Regulation Growth Hormone Insulin-Like Growth Factor I Liver Molecular Sequence Data Muscle, Skeletal Real-Time Polymerase Chain Reaction Receptors, Somatotropin RNA, Messenger Sequence Analysis, DNA Cichlasoma dimerus is a social cichlid fish capable of growing at high rates under laboratory conditions, but knowledge on somatic growth regulation is still unclear. Growth hormone (GH)/insulin-like growth factor I (IGF-I) axis is the key regulator of somatic growth in vertebrates. Two types of growth hormone receptors have been described in teleost fish, named GH receptor type 1 (GHR1) and type 2 (GHR2). In addition, isoforms of these receptors lacking part of the intracellular region have been described. The aim of this study was to evaluate the somatic growth, liver histology and changes in the GH/IGF-I axis after 4 weeks of food deprivation in C. dimerus. Four-week fasted fish showed reductions in specific growth rates in body weight (p < 0.001) and standard length (p < 0.001). Additionally, the hepatosomatic index (p < 0.001) and hepatocyte area (p < 0.001) decreased in fasted fish, while no changes in glucose levels were detected in plasma. The starvation protocol failed to induce changes in GH mRNA levels in the pituitary and IGF-I mRNA levels in liver. In contrast, IGF-I mRNA levels in muscle decreased in fasted fish (p = 0.002). On the other hand, GHR2 (detected with primer sets designed over the extracellular and intracellular region) was upregulated by starvation both in liver and muscle (p < 0.05), while GHR1 remained unchanged. These results show that a fasting period reduced somatic growth both in length and body weight concomitantly with alterations on liver and muscle GHR2 and muscle IGF-I mRNA expression. © 2014, Springer Science+Business Media Dordrecht. Fil:Di Yorio, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vissio, P.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2014 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09201742_v41_n1_p51_Delgadin http://hdl.handle.net/20.500.12110/paper_09201742_v41_n1_p51_Delgadin |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cichlid GHR1 GHR2 Growth hormone IGF-I growth hormone growth hormone receptor messenger RNA primer DNA somatomedin C analysis of variance animal body size cichlid DNA sequence food deprivation gene expression regulation genetics growth, development and aging liver metabolism molecular genetics nucleotide sequence physiology real time polymerase chain reaction skeletal muscle Analysis of Variance Animals Base Sequence Body Size Cichlids DNA Primers Food Deprivation Gene Expression Regulation Growth Hormone Insulin-Like Growth Factor I Liver Molecular Sequence Data Muscle, Skeletal Real-Time Polymerase Chain Reaction Receptors, Somatotropin RNA, Messenger Sequence Analysis, DNA |
| spellingShingle |
Cichlid GHR1 GHR2 Growth hormone IGF-I growth hormone growth hormone receptor messenger RNA primer DNA somatomedin C analysis of variance animal body size cichlid DNA sequence food deprivation gene expression regulation genetics growth, development and aging liver metabolism molecular genetics nucleotide sequence physiology real time polymerase chain reaction skeletal muscle Analysis of Variance Animals Base Sequence Body Size Cichlids DNA Primers Food Deprivation Gene Expression Regulation Growth Hormone Insulin-Like Growth Factor I Liver Molecular Sequence Data Muscle, Skeletal Real-Time Polymerase Chain Reaction Receptors, Somatotropin RNA, Messenger Sequence Analysis, DNA Di Yorio, María Paula Vissio, Paula Gabriela GH, IGF-I and GH receptors mRNA expression in response to growth impairment following a food deprivation period in individually housed cichlid fish Cichlasoma dimerus |
| topic_facet |
Cichlid GHR1 GHR2 Growth hormone IGF-I growth hormone growth hormone receptor messenger RNA primer DNA somatomedin C analysis of variance animal body size cichlid DNA sequence food deprivation gene expression regulation genetics growth, development and aging liver metabolism molecular genetics nucleotide sequence physiology real time polymerase chain reaction skeletal muscle Analysis of Variance Animals Base Sequence Body Size Cichlids DNA Primers Food Deprivation Gene Expression Regulation Growth Hormone Insulin-Like Growth Factor I Liver Molecular Sequence Data Muscle, Skeletal Real-Time Polymerase Chain Reaction Receptors, Somatotropin RNA, Messenger Sequence Analysis, DNA |
| description |
Cichlasoma dimerus is a social cichlid fish capable of growing at high rates under laboratory conditions, but knowledge on somatic growth regulation is still unclear. Growth hormone (GH)/insulin-like growth factor I (IGF-I) axis is the key regulator of somatic growth in vertebrates. Two types of growth hormone receptors have been described in teleost fish, named GH receptor type 1 (GHR1) and type 2 (GHR2). In addition, isoforms of these receptors lacking part of the intracellular region have been described. The aim of this study was to evaluate the somatic growth, liver histology and changes in the GH/IGF-I axis after 4 weeks of food deprivation in C. dimerus. Four-week fasted fish showed reductions in specific growth rates in body weight (p < 0.001) and standard length (p < 0.001). Additionally, the hepatosomatic index (p < 0.001) and hepatocyte area (p < 0.001) decreased in fasted fish, while no changes in glucose levels were detected in plasma. The starvation protocol failed to induce changes in GH mRNA levels in the pituitary and IGF-I mRNA levels in liver. In contrast, IGF-I mRNA levels in muscle decreased in fasted fish (p = 0.002). On the other hand, GHR2 (detected with primer sets designed over the extracellular and intracellular region) was upregulated by starvation both in liver and muscle (p < 0.05), while GHR1 remained unchanged. These results show that a fasting period reduced somatic growth both in length and body weight concomitantly with alterations on liver and muscle GHR2 and muscle IGF-I mRNA expression. © 2014, Springer Science+Business Media Dordrecht. |
| author |
Di Yorio, María Paula Vissio, Paula Gabriela |
| author_facet |
Di Yorio, María Paula Vissio, Paula Gabriela |
| author_sort |
Di Yorio, María Paula |
| title |
GH, IGF-I and GH receptors mRNA expression in response to growth impairment following a food deprivation period in individually housed cichlid fish Cichlasoma dimerus |
| title_short |
GH, IGF-I and GH receptors mRNA expression in response to growth impairment following a food deprivation period in individually housed cichlid fish Cichlasoma dimerus |
| title_full |
GH, IGF-I and GH receptors mRNA expression in response to growth impairment following a food deprivation period in individually housed cichlid fish Cichlasoma dimerus |
| title_fullStr |
GH, IGF-I and GH receptors mRNA expression in response to growth impairment following a food deprivation period in individually housed cichlid fish Cichlasoma dimerus |
| title_full_unstemmed |
GH, IGF-I and GH receptors mRNA expression in response to growth impairment following a food deprivation period in individually housed cichlid fish Cichlasoma dimerus |
| title_sort |
gh, igf-i and gh receptors mrna expression in response to growth impairment following a food deprivation period in individually housed cichlid fish cichlasoma dimerus |
| publishDate |
2014 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09201742_v41_n1_p51_Delgadin http://hdl.handle.net/20.500.12110/paper_09201742_v41_n1_p51_Delgadin |
| work_keys_str_mv |
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| _version_ |
1768542745904807936 |