Gonadotropin-releasing hormone (GnRH) neuronal systems in the pejerrey, Odontesthes bonariensis (Atheriniformes)
The brain of the pejerrey (Odontesthes bonariensis) has recently been shown to contain three forms of gonadotropin-releasing hormone (GnRH): salmon GnRH (sGnRH), chicken GnRH-II (cGnRH-II) and pejerrey GnRH (pjGnRH), nevertheless neuroanatomical studies on the distribution of these peptides are lack...
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2000
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09201742_v23_n3_p215_Stefano http://hdl.handle.net/20.500.12110/paper_09201742_v23_n3_p215_Stefano |
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paper:paper_09201742_v23_n3_p215_Stefano2023-06-08T15:50:03Z Gonadotropin-releasing hormone (GnRH) neuronal systems in the pejerrey, Odontesthes bonariensis (Atheriniformes) GnRH Immunohistochemistry Midbrain Pejerrey Preoptic area Terminal nerve antiserum gonadorelin monoclonal antibody animal cell animal tissue article brain level fish forebrain ganglion hypophysis hypothalamus immunoreactivity mesencephalon nerve fiber nonhuman Odontesthes bonariensis olfactory bulb preoptic area protein content protein expression tegmentum telencephalon tissue distribution Animalia Atheriniformes Gallus gallus Odontesthes bonariensis Vertebrata The brain of the pejerrey (Odontesthes bonariensis) has recently been shown to contain three forms of gonadotropin-releasing hormone (GnRH): salmon GnRH (sGnRH), chicken GnRH-II (cGnRH-II) and pejerrey GnRH (pjGnRH), nevertheless neuroanatomical studies on the distribution of these peptides are lacking. In this study we investigated the distribution of immunoreactive GnRH in the brain of adult pejerrey. Four different policlonal antisera and a monoclonal antibody against different GnRH variants were applied on cryosections and visualized using the ABC method. Three antisera (PBL#49, sGnRH#2 and cII741) revealed three different immunoreactive areas: the terminal nerve ganglion (at the junction between the olfactory bulbs and the anterior telencephalon), the preoptic area just anterior to the hypothalamus and the midbrain tegmentum. Fibers immunoreactive to GnRH were detected in different brain areas: the olfactory bulbs, the ventral thelencephalon, the hypothalamus, the mesencephalic area and an important innervation entering into the pituitary gland. Two other antibodies (LRH13 and s1668) labeled the two nuclei corresponding to the forebrain but not the midbrain tegmentum. As both antibodies have low crossreactivity to cGnRH-II, the data suggest that this group of cells express cGnRH-II. In summary, three different areas with immunoreactivity to GnRH were detected in the pejerrey brain. The distribution of sGnRH, pjGnRH and cGnRH-II expressing neurons, is discussed. 2000 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09201742_v23_n3_p215_Stefano http://hdl.handle.net/20.500.12110/paper_09201742_v23_n3_p215_Stefano |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
GnRH Immunohistochemistry Midbrain Pejerrey Preoptic area Terminal nerve antiserum gonadorelin monoclonal antibody animal cell animal tissue article brain level fish forebrain ganglion hypophysis hypothalamus immunoreactivity mesencephalon nerve fiber nonhuman Odontesthes bonariensis olfactory bulb preoptic area protein content protein expression tegmentum telencephalon tissue distribution Animalia Atheriniformes Gallus gallus Odontesthes bonariensis Vertebrata |
| spellingShingle |
GnRH Immunohistochemistry Midbrain Pejerrey Preoptic area Terminal nerve antiserum gonadorelin monoclonal antibody animal cell animal tissue article brain level fish forebrain ganglion hypophysis hypothalamus immunoreactivity mesencephalon nerve fiber nonhuman Odontesthes bonariensis olfactory bulb preoptic area protein content protein expression tegmentum telencephalon tissue distribution Animalia Atheriniformes Gallus gallus Odontesthes bonariensis Vertebrata Gonadotropin-releasing hormone (GnRH) neuronal systems in the pejerrey, Odontesthes bonariensis (Atheriniformes) |
| topic_facet |
GnRH Immunohistochemistry Midbrain Pejerrey Preoptic area Terminal nerve antiserum gonadorelin monoclonal antibody animal cell animal tissue article brain level fish forebrain ganglion hypophysis hypothalamus immunoreactivity mesencephalon nerve fiber nonhuman Odontesthes bonariensis olfactory bulb preoptic area protein content protein expression tegmentum telencephalon tissue distribution Animalia Atheriniformes Gallus gallus Odontesthes bonariensis Vertebrata |
| description |
The brain of the pejerrey (Odontesthes bonariensis) has recently been shown to contain three forms of gonadotropin-releasing hormone (GnRH): salmon GnRH (sGnRH), chicken GnRH-II (cGnRH-II) and pejerrey GnRH (pjGnRH), nevertheless neuroanatomical studies on the distribution of these peptides are lacking. In this study we investigated the distribution of immunoreactive GnRH in the brain of adult pejerrey. Four different policlonal antisera and a monoclonal antibody against different GnRH variants were applied on cryosections and visualized using the ABC method. Three antisera (PBL#49, sGnRH#2 and cII741) revealed three different immunoreactive areas: the terminal nerve ganglion (at the junction between the olfactory bulbs and the anterior telencephalon), the preoptic area just anterior to the hypothalamus and the midbrain tegmentum. Fibers immunoreactive to GnRH were detected in different brain areas: the olfactory bulbs, the ventral thelencephalon, the hypothalamus, the mesencephalic area and an important innervation entering into the pituitary gland. Two other antibodies (LRH13 and s1668) labeled the two nuclei corresponding to the forebrain but not the midbrain tegmentum. As both antibodies have low crossreactivity to cGnRH-II, the data suggest that this group of cells express cGnRH-II. In summary, three different areas with immunoreactivity to GnRH were detected in the pejerrey brain. The distribution of sGnRH, pjGnRH and cGnRH-II expressing neurons, is discussed. |
| title |
Gonadotropin-releasing hormone (GnRH) neuronal systems in the pejerrey, Odontesthes bonariensis (Atheriniformes) |
| title_short |
Gonadotropin-releasing hormone (GnRH) neuronal systems in the pejerrey, Odontesthes bonariensis (Atheriniformes) |
| title_full |
Gonadotropin-releasing hormone (GnRH) neuronal systems in the pejerrey, Odontesthes bonariensis (Atheriniformes) |
| title_fullStr |
Gonadotropin-releasing hormone (GnRH) neuronal systems in the pejerrey, Odontesthes bonariensis (Atheriniformes) |
| title_full_unstemmed |
Gonadotropin-releasing hormone (GnRH) neuronal systems in the pejerrey, Odontesthes bonariensis (Atheriniformes) |
| title_sort |
gonadotropin-releasing hormone (gnrh) neuronal systems in the pejerrey, odontesthes bonariensis (atheriniformes) |
| publishDate |
2000 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09201742_v23_n3_p215_Stefano http://hdl.handle.net/20.500.12110/paper_09201742_v23_n3_p215_Stefano |
| _version_ |
1768545745269424128 |