Effects of staurosporine on ACTH-mediated stimulation of aldosterone production
Incubation of rat adrenal glomerulosa cells with low concentrations (up to 50 nM) of the protein kinase (PKC) inhibitor staurosporine (ST) inhibited aldosterone (ALDO) and cyclic AMP (cAMP) production stimulated by adrenocorticotropic hormone (ACTH) and cholera toxin. Only higher concentrations (1.6...
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1996
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08986568_v8_n5_p381_Pecci http://hdl.handle.net/20.500.12110/paper_08986568_v8_n5_p381_Pecci |
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paper:paper_08986568_v8_n5_p381_Pecci2023-06-08T15:49:28Z Effects of staurosporine on ACTH-mediated stimulation of aldosterone production Pecci, Adali Romero, Damián Gastón Cozza, Eduardo Néstor Adrenocorticotropin Aldosterone Staurosporine aldosterone bucladesine cholera toxin corticotropin cyclic AMP cyclic AMP dependent protein kinase isobutylmethylxanthine protein kinase protein kinase C inhibitor staurosporine stimulatory guanine nucleotide binding protein animal cell animal tissue article controlled study enzyme inhibition hormone synthesis male nonhuman priority journal rat second messenger zona glomerulosa Animalia Incubation of rat adrenal glomerulosa cells with low concentrations (up to 50 nM) of the protein kinase (PKC) inhibitor staurosporine (ST) inhibited aldosterone (ALDO) and cyclic AMP (cAMP) production stimulated by adrenocorticotropic hormone (ACTH) and cholera toxin. Only higher concentrations (1.6 μM) of staurosporine inhibited dibutyryl-cAMP-and forskolin-induced stimulation of aldosterone production. cAMP levels were increased only with low concentrations of the PKC inhibitor. This latter increase was avoided by treatment with a maximal concentration of isobutylmethylxanthine (MIX). Our results suggest that: (1) second messengers other than cAMP are involved in ACTH action; (2) staurosporine inhibits different kinases involved in ACTH action in a dose dependent manner; (3) the protein kinase inhibited by high concentrations of staurosporine appears to be the cAMP dependent kinase, PKA; and (4) the protein kinase inhibited by low concentrations of staurosporine remains to be identified. This latter species is suggested as being involved in mediating ACTH-induced activation of Gs. Fil:Pecci, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Romero, D.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cozza, E.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08986568_v8_n5_p381_Pecci http://hdl.handle.net/20.500.12110/paper_08986568_v8_n5_p381_Pecci |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Adrenocorticotropin Aldosterone Staurosporine aldosterone bucladesine cholera toxin corticotropin cyclic AMP cyclic AMP dependent protein kinase isobutylmethylxanthine protein kinase protein kinase C inhibitor staurosporine stimulatory guanine nucleotide binding protein animal cell animal tissue article controlled study enzyme inhibition hormone synthesis male nonhuman priority journal rat second messenger zona glomerulosa Animalia |
spellingShingle |
Adrenocorticotropin Aldosterone Staurosporine aldosterone bucladesine cholera toxin corticotropin cyclic AMP cyclic AMP dependent protein kinase isobutylmethylxanthine protein kinase protein kinase C inhibitor staurosporine stimulatory guanine nucleotide binding protein animal cell animal tissue article controlled study enzyme inhibition hormone synthesis male nonhuman priority journal rat second messenger zona glomerulosa Animalia Pecci, Adali Romero, Damián Gastón Cozza, Eduardo Néstor Effects of staurosporine on ACTH-mediated stimulation of aldosterone production |
topic_facet |
Adrenocorticotropin Aldosterone Staurosporine aldosterone bucladesine cholera toxin corticotropin cyclic AMP cyclic AMP dependent protein kinase isobutylmethylxanthine protein kinase protein kinase C inhibitor staurosporine stimulatory guanine nucleotide binding protein animal cell animal tissue article controlled study enzyme inhibition hormone synthesis male nonhuman priority journal rat second messenger zona glomerulosa Animalia |
description |
Incubation of rat adrenal glomerulosa cells with low concentrations (up to 50 nM) of the protein kinase (PKC) inhibitor staurosporine (ST) inhibited aldosterone (ALDO) and cyclic AMP (cAMP) production stimulated by adrenocorticotropic hormone (ACTH) and cholera toxin. Only higher concentrations (1.6 μM) of staurosporine inhibited dibutyryl-cAMP-and forskolin-induced stimulation of aldosterone production. cAMP levels were increased only with low concentrations of the PKC inhibitor. This latter increase was avoided by treatment with a maximal concentration of isobutylmethylxanthine (MIX). Our results suggest that: (1) second messengers other than cAMP are involved in ACTH action; (2) staurosporine inhibits different kinases involved in ACTH action in a dose dependent manner; (3) the protein kinase inhibited by high concentrations of staurosporine appears to be the cAMP dependent kinase, PKA; and (4) the protein kinase inhibited by low concentrations of staurosporine remains to be identified. This latter species is suggested as being involved in mediating ACTH-induced activation of Gs. |
author |
Pecci, Adali Romero, Damián Gastón Cozza, Eduardo Néstor |
author_facet |
Pecci, Adali Romero, Damián Gastón Cozza, Eduardo Néstor |
author_sort |
Pecci, Adali |
title |
Effects of staurosporine on ACTH-mediated stimulation of aldosterone production |
title_short |
Effects of staurosporine on ACTH-mediated stimulation of aldosterone production |
title_full |
Effects of staurosporine on ACTH-mediated stimulation of aldosterone production |
title_fullStr |
Effects of staurosporine on ACTH-mediated stimulation of aldosterone production |
title_full_unstemmed |
Effects of staurosporine on ACTH-mediated stimulation of aldosterone production |
title_sort |
effects of staurosporine on acth-mediated stimulation of aldosterone production |
publishDate |
1996 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08986568_v8_n5_p381_Pecci http://hdl.handle.net/20.500.12110/paper_08986568_v8_n5_p381_Pecci |
work_keys_str_mv |
AT pecciadali effectsofstaurosporineonacthmediatedstimulationofaldosteroneproduction AT romerodamiangaston effectsofstaurosporineonacthmediatedstimulationofaldosteroneproduction AT cozzaeduardonestor effectsofstaurosporineonacthmediatedstimulationofaldosteroneproduction |
_version_ |
1768541995426381824 |