Effects of Progesterone on oligodendrocyte progenitors, oligodendrocyte transcription factors, and myelin proteins following spinal cord injury
Progesterone is emerging as a myelinizing factor for central nervous system injury. Successful remyelination requires proliferation and differentiation of oligodendrocyte precursor cells (OPC) into myelinating oligodendrocytes, but this process is incomplete following injury. To study progesterone a...
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2009
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08941491_v57_n8_p884_Labombarda http://hdl.handle.net/20.500.12110/paper_08941491_v57_n8_p884_Labombarda |
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paper:paper_08941491_v57_n8_p884_Labombarda2023-06-08T15:47:40Z Effects of Progesterone on oligodendrocyte progenitors, oligodendrocyte transcription factors, and myelin proteins following spinal cord injury NG2 cells Nkx2.2 Olig1 Olig2 Remyelination Steroids Trauma broxuridine messenger RNA myelin basic protein myelin oligodendrocyte glycoprotein myelin protein nucleic acid binding protein oligodendrocyte transcription factor 1 oligodendrocyte transcription factor 2 progesterone proteolipid protein transcription factor transcription factor Nkx2.2 unclassified drug basic helix loop helix transcription factor gestagen homeodomain protein messenger RNA myelin associated glycoprotein myelin protein nerve protein Nkx 2.2 homedomain protein Nkx-2.2 homedomain protein Olig1 protein, rat oligodendrocyte myelin glycoprotein oligodendrocyte-myelin glycoprotein Plp1 protein, rat progesterone proteolipid protein transcription factor animal cell animal experiment animal model article cell count cell density cell function cell lineage cell proliferation cell specificity chronic drug administration controlled study demyelination down regulation drug mechanism early intervention gene expression regulation hormone action male nerve cell differentiation neural stem cell nonhuman nucleotide sequence oligodendroglia priority journal protein expression protein function rat remyelinization short course therapy spinal cord injury upregulation adult stem cell animal disease model drug effect genetics metabolism methodology oligodendroglia orchiectomy Sprague Dawley rat time Adult Stem Cells Animals Basic Helix-Loop-Helix Transcription Factors Disease Models, Animal Gene Expression Regulation Homeodomain Proteins Male Myelin Proteins Myelin Proteolipid Protein Myelin-Associated Glycoprotein Nerve Tissue Proteins Oligodendroglia Orchiectomy Progesterone Progestins Rats Rats, Sprague-Dawley RNA, Messenger Spinal Cord Injuries Time Factors Transcription Factors Progesterone is emerging as a myelinizing factor for central nervous system injury. Successful remyelination requires proliferation and differentiation of oligodendrocyte precursor cells (OPC) into myelinating oligodendrocytes, but this process is incomplete following injury. To study progesterone actions on remyelination, we administered progesterone (16 mg/kg/day) to rats with complete spinal cord injury. Rats were euthanized 3 or 21 days after steroid treatment. Short progesterone treatment (a) increased the number of OPC without effect on the injury-induced reduction of mature oligodendrocytes, (b) increased mRNA and protein expression for the myelin basic protein (MBP) without effects on proteolipid protein (PLP) or myelin oligodendrocyte glycoprotein (MOG), and (c) increased the mRNA for Olig2 and Nkx2.2 transcription factors involved in specification and differentiation of the oligodendrocyte lineage. Furthermore, long progesterone treatment (a) reduced OPC with a concomitant increase of oligodendrocytes; (b) promoted differentiation of cells that incorporated bromodeoxyuridine, early after injury, into mature oligodendrocytes; (c) increased mRNA and protein expression of PLP without effects on MBP or MOG; and (d) increased mRNA for the Olig1 transcription factor involved in myelin repair. These results suggest that early progesterone treatment enhanced the density of OPC and induced their differentiation into mature oligodendrocytes by increasing the expression of Olig2 and Nkx2.2. Twenty-one days after injury, progesterone favors remyelination by increasing Olig1 (involved in repair of demyelinated lesions), PLP expression, and enhancing oligodendrocytes maturation. Thus, progesterone effects on oligodendrogenesis and myelin proteins may constitute fundamental steps for repairing traumatic injury inflicted to the spinal cord. © 2008 Wiley-Liss, Inc. 2009 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08941491_v57_n8_p884_Labombarda http://hdl.handle.net/20.500.12110/paper_08941491_v57_n8_p884_Labombarda |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
NG2 cells Nkx2.2 Olig1 Olig2 Remyelination Steroids Trauma broxuridine messenger RNA myelin basic protein myelin oligodendrocyte glycoprotein myelin protein nucleic acid binding protein oligodendrocyte transcription factor 1 oligodendrocyte transcription factor 2 progesterone proteolipid protein transcription factor transcription factor Nkx2.2 unclassified drug basic helix loop helix transcription factor gestagen homeodomain protein messenger RNA myelin associated glycoprotein myelin protein nerve protein Nkx 2.2 homedomain protein Nkx-2.2 homedomain protein Olig1 protein, rat oligodendrocyte myelin glycoprotein oligodendrocyte-myelin glycoprotein Plp1 protein, rat progesterone proteolipid protein transcription factor animal cell animal experiment animal model article cell count cell density cell function cell lineage cell proliferation cell specificity chronic drug administration controlled study demyelination down regulation drug mechanism early intervention gene expression regulation hormone action male nerve cell differentiation neural stem cell nonhuman nucleotide sequence oligodendroglia priority journal protein expression protein function rat remyelinization short course therapy spinal cord injury upregulation adult stem cell animal disease model drug effect genetics metabolism methodology oligodendroglia orchiectomy Sprague Dawley rat time Adult Stem Cells Animals Basic Helix-Loop-Helix Transcription Factors Disease Models, Animal Gene Expression Regulation Homeodomain Proteins Male Myelin Proteins Myelin Proteolipid Protein Myelin-Associated Glycoprotein Nerve Tissue Proteins Oligodendroglia Orchiectomy Progesterone Progestins Rats Rats, Sprague-Dawley RNA, Messenger Spinal Cord Injuries Time Factors Transcription Factors |
| spellingShingle |
NG2 cells Nkx2.2 Olig1 Olig2 Remyelination Steroids Trauma broxuridine messenger RNA myelin basic protein myelin oligodendrocyte glycoprotein myelin protein nucleic acid binding protein oligodendrocyte transcription factor 1 oligodendrocyte transcription factor 2 progesterone proteolipid protein transcription factor transcription factor Nkx2.2 unclassified drug basic helix loop helix transcription factor gestagen homeodomain protein messenger RNA myelin associated glycoprotein myelin protein nerve protein Nkx 2.2 homedomain protein Nkx-2.2 homedomain protein Olig1 protein, rat oligodendrocyte myelin glycoprotein oligodendrocyte-myelin glycoprotein Plp1 protein, rat progesterone proteolipid protein transcription factor animal cell animal experiment animal model article cell count cell density cell function cell lineage cell proliferation cell specificity chronic drug administration controlled study demyelination down regulation drug mechanism early intervention gene expression regulation hormone action male nerve cell differentiation neural stem cell nonhuman nucleotide sequence oligodendroglia priority journal protein expression protein function rat remyelinization short course therapy spinal cord injury upregulation adult stem cell animal disease model drug effect genetics metabolism methodology oligodendroglia orchiectomy Sprague Dawley rat time Adult Stem Cells Animals Basic Helix-Loop-Helix Transcription Factors Disease Models, Animal Gene Expression Regulation Homeodomain Proteins Male Myelin Proteins Myelin Proteolipid Protein Myelin-Associated Glycoprotein Nerve Tissue Proteins Oligodendroglia Orchiectomy Progesterone Progestins Rats Rats, Sprague-Dawley RNA, Messenger Spinal Cord Injuries Time Factors Transcription Factors Effects of Progesterone on oligodendrocyte progenitors, oligodendrocyte transcription factors, and myelin proteins following spinal cord injury |
| topic_facet |
NG2 cells Nkx2.2 Olig1 Olig2 Remyelination Steroids Trauma broxuridine messenger RNA myelin basic protein myelin oligodendrocyte glycoprotein myelin protein nucleic acid binding protein oligodendrocyte transcription factor 1 oligodendrocyte transcription factor 2 progesterone proteolipid protein transcription factor transcription factor Nkx2.2 unclassified drug basic helix loop helix transcription factor gestagen homeodomain protein messenger RNA myelin associated glycoprotein myelin protein nerve protein Nkx 2.2 homedomain protein Nkx-2.2 homedomain protein Olig1 protein, rat oligodendrocyte myelin glycoprotein oligodendrocyte-myelin glycoprotein Plp1 protein, rat progesterone proteolipid protein transcription factor animal cell animal experiment animal model article cell count cell density cell function cell lineage cell proliferation cell specificity chronic drug administration controlled study demyelination down regulation drug mechanism early intervention gene expression regulation hormone action male nerve cell differentiation neural stem cell nonhuman nucleotide sequence oligodendroglia priority journal protein expression protein function rat remyelinization short course therapy spinal cord injury upregulation adult stem cell animal disease model drug effect genetics metabolism methodology oligodendroglia orchiectomy Sprague Dawley rat time Adult Stem Cells Animals Basic Helix-Loop-Helix Transcription Factors Disease Models, Animal Gene Expression Regulation Homeodomain Proteins Male Myelin Proteins Myelin Proteolipid Protein Myelin-Associated Glycoprotein Nerve Tissue Proteins Oligodendroglia Orchiectomy Progesterone Progestins Rats Rats, Sprague-Dawley RNA, Messenger Spinal Cord Injuries Time Factors Transcription Factors |
| description |
Progesterone is emerging as a myelinizing factor for central nervous system injury. Successful remyelination requires proliferation and differentiation of oligodendrocyte precursor cells (OPC) into myelinating oligodendrocytes, but this process is incomplete following injury. To study progesterone actions on remyelination, we administered progesterone (16 mg/kg/day) to rats with complete spinal cord injury. Rats were euthanized 3 or 21 days after steroid treatment. Short progesterone treatment (a) increased the number of OPC without effect on the injury-induced reduction of mature oligodendrocytes, (b) increased mRNA and protein expression for the myelin basic protein (MBP) without effects on proteolipid protein (PLP) or myelin oligodendrocyte glycoprotein (MOG), and (c) increased the mRNA for Olig2 and Nkx2.2 transcription factors involved in specification and differentiation of the oligodendrocyte lineage. Furthermore, long progesterone treatment (a) reduced OPC with a concomitant increase of oligodendrocytes; (b) promoted differentiation of cells that incorporated bromodeoxyuridine, early after injury, into mature oligodendrocytes; (c) increased mRNA and protein expression of PLP without effects on MBP or MOG; and (d) increased mRNA for the Olig1 transcription factor involved in myelin repair. These results suggest that early progesterone treatment enhanced the density of OPC and induced their differentiation into mature oligodendrocytes by increasing the expression of Olig2 and Nkx2.2. Twenty-one days after injury, progesterone favors remyelination by increasing Olig1 (involved in repair of demyelinated lesions), PLP expression, and enhancing oligodendrocytes maturation. Thus, progesterone effects on oligodendrogenesis and myelin proteins may constitute fundamental steps for repairing traumatic injury inflicted to the spinal cord. © 2008 Wiley-Liss, Inc. |
| title |
Effects of Progesterone on oligodendrocyte progenitors, oligodendrocyte transcription factors, and myelin proteins following spinal cord injury |
| title_short |
Effects of Progesterone on oligodendrocyte progenitors, oligodendrocyte transcription factors, and myelin proteins following spinal cord injury |
| title_full |
Effects of Progesterone on oligodendrocyte progenitors, oligodendrocyte transcription factors, and myelin proteins following spinal cord injury |
| title_fullStr |
Effects of Progesterone on oligodendrocyte progenitors, oligodendrocyte transcription factors, and myelin proteins following spinal cord injury |
| title_full_unstemmed |
Effects of Progesterone on oligodendrocyte progenitors, oligodendrocyte transcription factors, and myelin proteins following spinal cord injury |
| title_sort |
effects of progesterone on oligodendrocyte progenitors, oligodendrocyte transcription factors, and myelin proteins following spinal cord injury |
| publishDate |
2009 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08941491_v57_n8_p884_Labombarda http://hdl.handle.net/20.500.12110/paper_08941491_v57_n8_p884_Labombarda |
| _version_ |
1768542894275166208 |