Nodule development induced by Mesorhizobium loti mutant strains affected in polysaccharide synthesis
The role of Mesorhizobium loti surface polysaccharides on the nodulation process is not yet fully understood. In this article, we describe the nodulation phenotype of mutants affected in the synthesis of lipopolysaccharide (LPS) and β(1,2) cyclic glucan. M. loti lpsβ2 mutant produces LPS with reduce...
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2005
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08940282_v18_n5_p446_DAntuono http://hdl.handle.net/20.500.12110/paper_08940282_v18_n5_p446_DAntuono |
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paper:paper_08940282_v18_n5_p446_DAntuono2023-06-08T15:47:39Z Nodule development induced by Mesorhizobium loti mutant strains affected in polysaccharide synthesis Epimerase/dehydratase family Glycosyltransferases LPS and β(1-2) cyclic glucan mutants calcium lipopolysaccharide article bacterial gene culture medium gene expression regulation genetics Lotus metabolism microbiology mutation phenotype physiology plant root Rhizobiaceae Calcium Culture Media Gene Expression Regulation, Bacterial Genes, Bacterial Lipopolysaccharides Lotus Mutation Phenotype Plant Roots Rhizobiaceae Lotus tenuis Mesorhizobium loti The role of Mesorhizobium loti surface polysaccharides on the nodulation process is not yet fully understood. In this article, we describe the nodulation phenotype of mutants affected in the synthesis of lipopolysaccharide (LPS) and β(1,2) cyclic glucan. M. loti lpsβ2 mutant produces LPS with reduced amount of O-antigen, whereas M. loti lpsβ1 mutant produces LPS totally devoid of O-antigen. Both genes are clustered in the chromosome. Based on amino acid sequence homology, LPS sugar composition, and enzymatic activity, we concluded that lpsβ2 codes for an enzyme involved in the transformation of dTDP-glucose into dTDP-rhamnose, the sugar donor of rhamnose for the synthesis of O-antigen. On the other hand, Ipsβ1 codes for a glucosyltransferase involved in the biosynthesis of the O-antigen. Although LPS mutants elicited normal nodules, both show reduced competitiveness compared with the wild type. M. loti β(1-2) cyclic glucan synthase (cgs) mutant induces white, empty, ineffective pseudonodules in Lotus tenuis. Cgs mutant induces normal root hair curling but is unable to induce the formation of infection threads. M. loti cgs mutant was more sensitive to deoxycholate and displayed motility impairment compared with the wild-type strain. This pleiotropic effect depends on calcium concentration and temperature. © 2005 The American Phytopathological Society. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08940282_v18_n5_p446_DAntuono http://hdl.handle.net/20.500.12110/paper_08940282_v18_n5_p446_DAntuono |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Epimerase/dehydratase family Glycosyltransferases LPS and β(1-2) cyclic glucan mutants calcium lipopolysaccharide article bacterial gene culture medium gene expression regulation genetics Lotus metabolism microbiology mutation phenotype physiology plant root Rhizobiaceae Calcium Culture Media Gene Expression Regulation, Bacterial Genes, Bacterial Lipopolysaccharides Lotus Mutation Phenotype Plant Roots Rhizobiaceae Lotus tenuis Mesorhizobium loti |
| spellingShingle |
Epimerase/dehydratase family Glycosyltransferases LPS and β(1-2) cyclic glucan mutants calcium lipopolysaccharide article bacterial gene culture medium gene expression regulation genetics Lotus metabolism microbiology mutation phenotype physiology plant root Rhizobiaceae Calcium Culture Media Gene Expression Regulation, Bacterial Genes, Bacterial Lipopolysaccharides Lotus Mutation Phenotype Plant Roots Rhizobiaceae Lotus tenuis Mesorhizobium loti Nodule development induced by Mesorhizobium loti mutant strains affected in polysaccharide synthesis |
| topic_facet |
Epimerase/dehydratase family Glycosyltransferases LPS and β(1-2) cyclic glucan mutants calcium lipopolysaccharide article bacterial gene culture medium gene expression regulation genetics Lotus metabolism microbiology mutation phenotype physiology plant root Rhizobiaceae Calcium Culture Media Gene Expression Regulation, Bacterial Genes, Bacterial Lipopolysaccharides Lotus Mutation Phenotype Plant Roots Rhizobiaceae Lotus tenuis Mesorhizobium loti |
| description |
The role of Mesorhizobium loti surface polysaccharides on the nodulation process is not yet fully understood. In this article, we describe the nodulation phenotype of mutants affected in the synthesis of lipopolysaccharide (LPS) and β(1,2) cyclic glucan. M. loti lpsβ2 mutant produces LPS with reduced amount of O-antigen, whereas M. loti lpsβ1 mutant produces LPS totally devoid of O-antigen. Both genes are clustered in the chromosome. Based on amino acid sequence homology, LPS sugar composition, and enzymatic activity, we concluded that lpsβ2 codes for an enzyme involved in the transformation of dTDP-glucose into dTDP-rhamnose, the sugar donor of rhamnose for the synthesis of O-antigen. On the other hand, Ipsβ1 codes for a glucosyltransferase involved in the biosynthesis of the O-antigen. Although LPS mutants elicited normal nodules, both show reduced competitiveness compared with the wild type. M. loti β(1-2) cyclic glucan synthase (cgs) mutant induces white, empty, ineffective pseudonodules in Lotus tenuis. Cgs mutant induces normal root hair curling but is unable to induce the formation of infection threads. M. loti cgs mutant was more sensitive to deoxycholate and displayed motility impairment compared with the wild-type strain. This pleiotropic effect depends on calcium concentration and temperature. © 2005 The American Phytopathological Society. |
| title |
Nodule development induced by Mesorhizobium loti mutant strains affected in polysaccharide synthesis |
| title_short |
Nodule development induced by Mesorhizobium loti mutant strains affected in polysaccharide synthesis |
| title_full |
Nodule development induced by Mesorhizobium loti mutant strains affected in polysaccharide synthesis |
| title_fullStr |
Nodule development induced by Mesorhizobium loti mutant strains affected in polysaccharide synthesis |
| title_full_unstemmed |
Nodule development induced by Mesorhizobium loti mutant strains affected in polysaccharide synthesis |
| title_sort |
nodule development induced by mesorhizobium loti mutant strains affected in polysaccharide synthesis |
| publishDate |
2005 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08940282_v18_n5_p446_DAntuono http://hdl.handle.net/20.500.12110/paper_08940282_v18_n5_p446_DAntuono |
| _version_ |
1768543474362089472 |