Biologic, Immunocytochemical, and Cytogenetic Characterization of Two New Human Melanoma Cell Lines: IIB‐MEL‐LES and IIB‐MEL‐IAN
Two human melanoma cell lines, derived from metastases of two patients with epithelioid malignant amelanotic melanomas, and designated IIB‐MEL‐LES and IIB‐MEL‐IAN, have been established. Both cell lines have been in continuous culture over 2 years and were propagated continuously for 85 and 75 seria...
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1995
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08935785_v8_n3_p121_KAIRIYAMA http://hdl.handle.net/20.500.12110/paper_08935785_v8_n3_p121_KAIRIYAMA |
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paper:paper_08935785_v8_n3_p121_KAIRIYAMA2023-06-08T15:47:33Z Biologic, Immunocytochemical, and Cytogenetic Characterization of Two New Human Melanoma Cell Lines: IIB‐MEL‐LES and IIB‐MEL‐IAN Antigens Cytogenetics MAGE PCR Melanoma cell lines DNA ganglioside ganglioside, GD2 ganglioside, GD3 HLA antigen class 1 HLA DR antigen protein S 100 restriction endonuclease vimentin adult amelanotic melanoma animal article cancer transplantation case report cell culture chemistry genetics human immunohistochemistry immunophenotyping karyotyping male metastasis molecular genetics mouse nucleotide sequence nude mouse pathology Adult Animals Base Sequence DNA Restriction Enzymes DNA, Neoplasm Gangliosides Histocompatibility Antigens Class I HLA-DR Antigens Humans Immunohistochemistry Immunophenotyping Karyotyping Male Melanoma, Amelanotic Mice Mice, Nude Molecular Sequence Data Neoplasm Metastasis Neoplasm Transplantation S100 Proteins Tumor Cells, Cultured Vimentin Two human melanoma cell lines, derived from metastases of two patients with epithelioid malignant amelanotic melanomas, and designated IIB‐MEL‐LES and IIB‐MEL‐IAN, have been established. Both cell lines have been in continuous culture over 2 years and were propagated continuously for 85 and 75 serial passages, respectively. Morphologically, IIB‐MEL‐LES is composed predominantly of spindle shaped cells, whereas IIB‐MEL‐IAN grows as a monolayer of cuboid and stellate shaped cells with many rounded cells in suspension. Immunocytochemical studies revealed that both cell lines express S‐100 protein, vimentin, and GD3 and GD2 gangliosides but are negative for keratin and collagen. Both cell lines express HLA class I and HLA‐DR antigens in variable proportions. The MAGE‐1 gene is expressed only by the IIB‐MEL‐IAN cell line, as revealed by PCR analysis. Cytogenetic analysis of both cell lines revealed abnormal karyotypes; the modal chromosome numbers of IIB‐MEL‐LES and IIB‐MEL‐IAN were 48 and 81, respectively. IIB‐MEL‐LES cells presented rearrangements in chromosomes 1, 14 and X, gains in chromosomes 10,20, and 21 losses in chromosomes 15 and Y. The most frequent markers observed in IIB‐MEL‐IAN cells were 7q+, 10p+, 2p+, i(6p), 2q+, and 10q‐. Clonal gains were observed in chromosomes 12 and 21, whereas losses were seen in chromosomes 1, 2, 3, 4, 6, 7, 11, and 17. Both cell lines were capable of forming colonies in soft agar and developed tumors when transplanted into nude mice, reproducing and maintaining the characteristics of the original tumors. These cell lines and their xenografts appear to provide useful systems for studying the biology, genetics and histogenesis of human malignant melanoma and could be utilized for the development of melanoma vaccines. Copyright © 1995, Wiley Blackwell. All rights reserved 1995 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08935785_v8_n3_p121_KAIRIYAMA http://hdl.handle.net/20.500.12110/paper_08935785_v8_n3_p121_KAIRIYAMA |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Antigens Cytogenetics MAGE PCR Melanoma cell lines DNA ganglioside ganglioside, GD2 ganglioside, GD3 HLA antigen class 1 HLA DR antigen protein S 100 restriction endonuclease vimentin adult amelanotic melanoma animal article cancer transplantation case report cell culture chemistry genetics human immunohistochemistry immunophenotyping karyotyping male metastasis molecular genetics mouse nucleotide sequence nude mouse pathology Adult Animals Base Sequence DNA Restriction Enzymes DNA, Neoplasm Gangliosides Histocompatibility Antigens Class I HLA-DR Antigens Humans Immunohistochemistry Immunophenotyping Karyotyping Male Melanoma, Amelanotic Mice Mice, Nude Molecular Sequence Data Neoplasm Metastasis Neoplasm Transplantation S100 Proteins Tumor Cells, Cultured Vimentin |
| spellingShingle |
Antigens Cytogenetics MAGE PCR Melanoma cell lines DNA ganglioside ganglioside, GD2 ganglioside, GD3 HLA antigen class 1 HLA DR antigen protein S 100 restriction endonuclease vimentin adult amelanotic melanoma animal article cancer transplantation case report cell culture chemistry genetics human immunohistochemistry immunophenotyping karyotyping male metastasis molecular genetics mouse nucleotide sequence nude mouse pathology Adult Animals Base Sequence DNA Restriction Enzymes DNA, Neoplasm Gangliosides Histocompatibility Antigens Class I HLA-DR Antigens Humans Immunohistochemistry Immunophenotyping Karyotyping Male Melanoma, Amelanotic Mice Mice, Nude Molecular Sequence Data Neoplasm Metastasis Neoplasm Transplantation S100 Proteins Tumor Cells, Cultured Vimentin Biologic, Immunocytochemical, and Cytogenetic Characterization of Two New Human Melanoma Cell Lines: IIB‐MEL‐LES and IIB‐MEL‐IAN |
| topic_facet |
Antigens Cytogenetics MAGE PCR Melanoma cell lines DNA ganglioside ganglioside, GD2 ganglioside, GD3 HLA antigen class 1 HLA DR antigen protein S 100 restriction endonuclease vimentin adult amelanotic melanoma animal article cancer transplantation case report cell culture chemistry genetics human immunohistochemistry immunophenotyping karyotyping male metastasis molecular genetics mouse nucleotide sequence nude mouse pathology Adult Animals Base Sequence DNA Restriction Enzymes DNA, Neoplasm Gangliosides Histocompatibility Antigens Class I HLA-DR Antigens Humans Immunohistochemistry Immunophenotyping Karyotyping Male Melanoma, Amelanotic Mice Mice, Nude Molecular Sequence Data Neoplasm Metastasis Neoplasm Transplantation S100 Proteins Tumor Cells, Cultured Vimentin |
| description |
Two human melanoma cell lines, derived from metastases of two patients with epithelioid malignant amelanotic melanomas, and designated IIB‐MEL‐LES and IIB‐MEL‐IAN, have been established. Both cell lines have been in continuous culture over 2 years and were propagated continuously for 85 and 75 serial passages, respectively. Morphologically, IIB‐MEL‐LES is composed predominantly of spindle shaped cells, whereas IIB‐MEL‐IAN grows as a monolayer of cuboid and stellate shaped cells with many rounded cells in suspension. Immunocytochemical studies revealed that both cell lines express S‐100 protein, vimentin, and GD3 and GD2 gangliosides but are negative for keratin and collagen. Both cell lines express HLA class I and HLA‐DR antigens in variable proportions. The MAGE‐1 gene is expressed only by the IIB‐MEL‐IAN cell line, as revealed by PCR analysis. Cytogenetic analysis of both cell lines revealed abnormal karyotypes; the modal chromosome numbers of IIB‐MEL‐LES and IIB‐MEL‐IAN were 48 and 81, respectively. IIB‐MEL‐LES cells presented rearrangements in chromosomes 1, 14 and X, gains in chromosomes 10,20, and 21 losses in chromosomes 15 and Y. The most frequent markers observed in IIB‐MEL‐IAN cells were 7q+, 10p+, 2p+, i(6p), 2q+, and 10q‐. Clonal gains were observed in chromosomes 12 and 21, whereas losses were seen in chromosomes 1, 2, 3, 4, 6, 7, 11, and 17. Both cell lines were capable of forming colonies in soft agar and developed tumors when transplanted into nude mice, reproducing and maintaining the characteristics of the original tumors. These cell lines and their xenografts appear to provide useful systems for studying the biology, genetics and histogenesis of human malignant melanoma and could be utilized for the development of melanoma vaccines. Copyright © 1995, Wiley Blackwell. All rights reserved |
| title |
Biologic, Immunocytochemical, and Cytogenetic Characterization of Two New Human Melanoma Cell Lines: IIB‐MEL‐LES and IIB‐MEL‐IAN |
| title_short |
Biologic, Immunocytochemical, and Cytogenetic Characterization of Two New Human Melanoma Cell Lines: IIB‐MEL‐LES and IIB‐MEL‐IAN |
| title_full |
Biologic, Immunocytochemical, and Cytogenetic Characterization of Two New Human Melanoma Cell Lines: IIB‐MEL‐LES and IIB‐MEL‐IAN |
| title_fullStr |
Biologic, Immunocytochemical, and Cytogenetic Characterization of Two New Human Melanoma Cell Lines: IIB‐MEL‐LES and IIB‐MEL‐IAN |
| title_full_unstemmed |
Biologic, Immunocytochemical, and Cytogenetic Characterization of Two New Human Melanoma Cell Lines: IIB‐MEL‐LES and IIB‐MEL‐IAN |
| title_sort |
biologic, immunocytochemical, and cytogenetic characterization of two new human melanoma cell lines: iib‐mel‐les and iib‐mel‐ian |
| publishDate |
1995 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08935785_v8_n3_p121_KAIRIYAMA http://hdl.handle.net/20.500.12110/paper_08935785_v8_n3_p121_KAIRIYAMA |
| _version_ |
1768541759056379904 |