Role of Melanin as a Scavenger of Active Oxygen Species

The protective role of melanin, either synthetic or derived from a metastatic lung melanoma nodule, was studied in terms of its ability to interact with active oxygen species (O2, H2O2, RO, ROO, etc.). Both melanins showed the ability to react with O2. The superoxide dismutase‐like activity correspo...

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Publicado:	1993
Materias:	Free Radical Lipid peroxidation Melanin Scavenger 2,2' azobis(2 amidinopropane) 2,2'-azobis(2-amidinopropane) amidine glucose oxidase melanin reactive oxygen metabolite scavenger superoxide dismutase animal article cell culture chemistry human lipid peroxidation melanoma metabolism pathology physiology Amidines Animal Free Radical Scavengers Glucose Oxidase Human Lipid Peroxidation Melanins Melanoma Reactive Oxygen Species Superoxide Dismutase Support, Non-U.S. Gov't Tumor Cells, Cultured
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08935785_v6_n5_p348_BUSTAMANTE http://hdl.handle.net/20.500.12110/paper_08935785_v6_n5_p348_BUSTAMANTE
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_08935785_v6_n5_p348_BUSTAMANTE
record_format	dspace
spelling	paper:paper_08935785_v6_n5_p348_BUSTAMANTE2023-06-08T15:47:33Z Role of Melanin as a Scavenger of Active Oxygen Species Free Radical Lipid peroxidation Melanin Scavenger 2,2' azobis(2 amidinopropane) 2,2'-azobis(2-amidinopropane) amidine glucose oxidase melanin reactive oxygen metabolite scavenger superoxide dismutase animal article cell culture chemistry human lipid peroxidation melanoma metabolism pathology physiology Amidines Animal Free Radical Scavengers Glucose Oxidase Human Lipid Peroxidation Melanins Melanoma Reactive Oxygen Species Superoxide Dismutase Support, Non-U.S. Gov't Tumor Cells, Cultured The protective role of melanin, either synthetic or derived from a metastatic lung melanoma nodule, was studied in terms of its ability to interact with active oxygen species (O2, H2O2, RO, ROO, etc.). Both melanins showed the ability to react with O2. The superoxide dismutase‐like activity corresponds to 21 and 10 U/mg for synthetic and tumor melanin, respectively. The latter value accounts for about 8% of the superoxide dismutase activity of cultured melanoma cells. Neither type of melanin showed catalase‐like or glutathione peroxidase‐like activity. Both types of melanin reacted with RO and ROO radicals as determined by inhibition of the lipid peroxidation reaction of rat liver homogenates. The spontaneous lipid peroxidation of rat liver homogenate was inhibited up to 90% and 80% by synthetic and tumor melanin with half‐maximal effects at 2.5 and 5.5 μg melanin/ml, respectively. The 2,2‐azobis‐(2 amidino propane) (AAPH)‐initiated lipid peroxidation of rat liver homogenate was inhibited up to 3% and 20% by synthetic and tumor melanin, with half maximal effect at 120 and 500 μg melanin/ml, respectively. Both types of melanin were able to protect the in vitro inactivation of glucose oxidase, which occurs in the presence of AAPH‐generated radicals. Copyright © 1993, Wiley Blackwell. All rights reserved 1993 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08935785_v6_n5_p348_BUSTAMANTE http://hdl.handle.net/20.500.12110/paper_08935785_v6_n5_p348_BUSTAMANTE
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Free Radical Lipid peroxidation Melanin Scavenger 2,2' azobis(2 amidinopropane) 2,2'-azobis(2-amidinopropane) amidine glucose oxidase melanin reactive oxygen metabolite scavenger superoxide dismutase animal article cell culture chemistry human lipid peroxidation melanoma metabolism pathology physiology Amidines Animal Free Radical Scavengers Glucose Oxidase Human Lipid Peroxidation Melanins Melanoma Reactive Oxygen Species Superoxide Dismutase Support, Non-U.S. Gov't Tumor Cells, Cultured
spellingShingle	Free Radical Lipid peroxidation Melanin Scavenger 2,2' azobis(2 amidinopropane) 2,2'-azobis(2-amidinopropane) amidine glucose oxidase melanin reactive oxygen metabolite scavenger superoxide dismutase animal article cell culture chemistry human lipid peroxidation melanoma metabolism pathology physiology Amidines Animal Free Radical Scavengers Glucose Oxidase Human Lipid Peroxidation Melanins Melanoma Reactive Oxygen Species Superoxide Dismutase Support, Non-U.S. Gov't Tumor Cells, Cultured Role of Melanin as a Scavenger of Active Oxygen Species
topic_facet	Free Radical Lipid peroxidation Melanin Scavenger 2,2' azobis(2 amidinopropane) 2,2'-azobis(2-amidinopropane) amidine glucose oxidase melanin reactive oxygen metabolite scavenger superoxide dismutase animal article cell culture chemistry human lipid peroxidation melanoma metabolism pathology physiology Amidines Animal Free Radical Scavengers Glucose Oxidase Human Lipid Peroxidation Melanins Melanoma Reactive Oxygen Species Superoxide Dismutase Support, Non-U.S. Gov't Tumor Cells, Cultured
description	The protective role of melanin, either synthetic or derived from a metastatic lung melanoma nodule, was studied in terms of its ability to interact with active oxygen species (O2, H2O2, RO, ROO, etc.). Both melanins showed the ability to react with O2. The superoxide dismutase‐like activity corresponds to 21 and 10 U/mg for synthetic and tumor melanin, respectively. The latter value accounts for about 8% of the superoxide dismutase activity of cultured melanoma cells. Neither type of melanin showed catalase‐like or glutathione peroxidase‐like activity. Both types of melanin reacted with RO and ROO radicals as determined by inhibition of the lipid peroxidation reaction of rat liver homogenates. The spontaneous lipid peroxidation of rat liver homogenate was inhibited up to 90% and 80% by synthetic and tumor melanin with half‐maximal effects at 2.5 and 5.5 μg melanin/ml, respectively. The 2,2‐azobis‐(2 amidino propane) (AAPH)‐initiated lipid peroxidation of rat liver homogenate was inhibited up to 3% and 20% by synthetic and tumor melanin, with half maximal effect at 120 and 500 μg melanin/ml, respectively. Both types of melanin were able to protect the in vitro inactivation of glucose oxidase, which occurs in the presence of AAPH‐generated radicals. Copyright © 1993, Wiley Blackwell. All rights reserved
title	Role of Melanin as a Scavenger of Active Oxygen Species
title_short	Role of Melanin as a Scavenger of Active Oxygen Species
title_full	Role of Melanin as a Scavenger of Active Oxygen Species
title_fullStr	Role of Melanin as a Scavenger of Active Oxygen Species
title_full_unstemmed	Role of Melanin as a Scavenger of Active Oxygen Species
title_sort	role of melanin as a scavenger of active oxygen species
publishDate	1993
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08935785_v6_n5_p348_BUSTAMANTE http://hdl.handle.net/20.500.12110/paper_08935785_v6_n5_p348_BUSTAMANTE
_version_	1768545838242463744

Role of Melanin as a Scavenger of Active Oxygen Species

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