Characterization of IIB‐MEL‐J: A New and Highly Heterogenous Human Melanoma Cell Line
A highly heterogeneous cell line, IIB‐MEL‐J, was established from a human metastatic melanoma. This cell line contains small cells, dendritic cells, and megacells with multiple nuclei. IIB‐MEL‐J expresses S 100, cytokeratin intermediate filaments and the gangliosides GD2 and GD3. It requires growth...
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1989
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08935785_v2_n6_p504_GUERRA http://hdl.handle.net/20.500.12110/paper_08935785_v2_n6_p504_GUERRA |
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paper:paper_08935785_v2_n6_p504_GUERRA2023-06-08T15:47:33Z Characterization of IIB‐MEL‐J: A New and Highly Heterogenous Human Melanoma Cell Line Guerra, Liliana Noemí Larripa, Irene Beatriz Cellular subpopulations Cytogenetics Growth factors adult article case report cell culture cell transformation chromosome chromosome aberration chromosome disorder electron microscopy human male melanoma pathology phenotype ultrastructure Case Report Cell Transformation, Neoplastic Chromosome Aberrations Chromosome Disorders Chromosomes Human Male Melanoma Microscopy, Electron Middle Age Phenotype Support, Non-U.S. Gov't Tumor Cells, Cultured A highly heterogeneous cell line, IIB‐MEL‐J, was established from a human metastatic melanoma. This cell line contains small cells, dendritic cells, and megacells with multiple nuclei. IIB‐MEL‐J expresses S 100, cytokeratin intermediate filaments and the gangliosides GD2 and GD3. It requires growth factors (insulin, EGF, and transferrin) and antioxidants for optimal growth. When plated under optimal conditions, IIB‐MEL‐J grows with a doubling time of 70–80 hours. The cells may be fractionated by Percoll gradient centrifugation into several subpopulations (A, B, and C) with different characteristics. Subpopulation A is the slowest growing, and most of the DNA‐synthesizing cells are concentrated in fractions B and C. Every subpopulation expresses S 100 and cytokeratin intermediate filaments, whereas only subpopulations B and C express GD2 and GD3. Pigmented cells are concentrated mainly in subpopulation C. Cytogenetic analysis of IIB‐MEL‐J revealed extensive chromosomal alterations, including a highly heterogeneous chromosome number and chromosomal rearrangements, gains, losses, isochromosomes, and double minutes. This highly heterogeneous cell line may be helpful to study cellular differentiation and interaction between different subpopulations in human melanoma. Copyright © 1989, Wiley Blackwell. All rights reserved Fil:GUERRA, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:LARRIPA, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1989 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08935785_v2_n6_p504_GUERRA http://hdl.handle.net/20.500.12110/paper_08935785_v2_n6_p504_GUERRA |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cellular subpopulations Cytogenetics Growth factors adult article case report cell culture cell transformation chromosome chromosome aberration chromosome disorder electron microscopy human male melanoma pathology phenotype ultrastructure Case Report Cell Transformation, Neoplastic Chromosome Aberrations Chromosome Disorders Chromosomes Human Male Melanoma Microscopy, Electron Middle Age Phenotype Support, Non-U.S. Gov't Tumor Cells, Cultured |
| spellingShingle |
Cellular subpopulations Cytogenetics Growth factors adult article case report cell culture cell transformation chromosome chromosome aberration chromosome disorder electron microscopy human male melanoma pathology phenotype ultrastructure Case Report Cell Transformation, Neoplastic Chromosome Aberrations Chromosome Disorders Chromosomes Human Male Melanoma Microscopy, Electron Middle Age Phenotype Support, Non-U.S. Gov't Tumor Cells, Cultured Guerra, Liliana Noemí Larripa, Irene Beatriz Characterization of IIB‐MEL‐J: A New and Highly Heterogenous Human Melanoma Cell Line |
| topic_facet |
Cellular subpopulations Cytogenetics Growth factors adult article case report cell culture cell transformation chromosome chromosome aberration chromosome disorder electron microscopy human male melanoma pathology phenotype ultrastructure Case Report Cell Transformation, Neoplastic Chromosome Aberrations Chromosome Disorders Chromosomes Human Male Melanoma Microscopy, Electron Middle Age Phenotype Support, Non-U.S. Gov't Tumor Cells, Cultured |
| description |
A highly heterogeneous cell line, IIB‐MEL‐J, was established from a human metastatic melanoma. This cell line contains small cells, dendritic cells, and megacells with multiple nuclei. IIB‐MEL‐J expresses S 100, cytokeratin intermediate filaments and the gangliosides GD2 and GD3. It requires growth factors (insulin, EGF, and transferrin) and antioxidants for optimal growth. When plated under optimal conditions, IIB‐MEL‐J grows with a doubling time of 70–80 hours. The cells may be fractionated by Percoll gradient centrifugation into several subpopulations (A, B, and C) with different characteristics. Subpopulation A is the slowest growing, and most of the DNA‐synthesizing cells are concentrated in fractions B and C. Every subpopulation expresses S 100 and cytokeratin intermediate filaments, whereas only subpopulations B and C express GD2 and GD3. Pigmented cells are concentrated mainly in subpopulation C. Cytogenetic analysis of IIB‐MEL‐J revealed extensive chromosomal alterations, including a highly heterogeneous chromosome number and chromosomal rearrangements, gains, losses, isochromosomes, and double minutes. This highly heterogeneous cell line may be helpful to study cellular differentiation and interaction between different subpopulations in human melanoma. Copyright © 1989, Wiley Blackwell. All rights reserved |
| author |
Guerra, Liliana Noemí Larripa, Irene Beatriz |
| author_facet |
Guerra, Liliana Noemí Larripa, Irene Beatriz |
| author_sort |
Guerra, Liliana Noemí |
| title |
Characterization of IIB‐MEL‐J: A New and Highly Heterogenous Human Melanoma Cell Line |
| title_short |
Characterization of IIB‐MEL‐J: A New and Highly Heterogenous Human Melanoma Cell Line |
| title_full |
Characterization of IIB‐MEL‐J: A New and Highly Heterogenous Human Melanoma Cell Line |
| title_fullStr |
Characterization of IIB‐MEL‐J: A New and Highly Heterogenous Human Melanoma Cell Line |
| title_full_unstemmed |
Characterization of IIB‐MEL‐J: A New and Highly Heterogenous Human Melanoma Cell Line |
| title_sort |
characterization of iib‐mel‐j: a new and highly heterogenous human melanoma cell line |
| publishDate |
1989 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08935785_v2_n6_p504_GUERRA http://hdl.handle.net/20.500.12110/paper_08935785_v2_n6_p504_GUERRA |
| work_keys_str_mv |
AT guerraliliananoemi characterizationofiibmeljanewandhighlyheterogenoushumanmelanomacellline AT larripairenebeatriz characterizationofiibmeljanewandhighlyheterogenoushumanmelanomacellline |
| _version_ |
1768544917300183040 |