The galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization

Lectin-glycan recognition systems play central roles in many physiologic and pathologic processes. We identified a role for galectin-1 (Gal-1), a highly conserved glycan-binding protein, in the control of sperm function. We found that Gal-1 is expressed in the epididymis and associates with sperm du...

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Autores principales:	Croci Russo, Diego Omar, Brukman, Nicolás G., Cuasnicú, Patricia S.
Publicado:	2015
Materias:	Acrosome reaction Capacitation Fertilization Glycosylation galectin 1 glycan progesterone polysaccharide recombinant protein acrosome reaction adult animal cell animal tissue Article cell volume controlled study cumulus cell enzyme linked immunosorbent assay epididymis female fertilization fertilization in vitro flow cytometry gamete genotype hyperpolarization immunoblotting in vitro study in vivo study male membrane fusion membrane hyperpolarization membrane potential mouse nonhuman oocyte ovary oviduct priority journal protein expression protein phosphorylation spermatozoon spermatozoon capacitation spermatozoon motility testis uterus young adult zygote animal C57BL mouse cell membrane cytology drug effects gene expression genetics knockout mouse metabolism physiology reverse transcription polymerase chain reaction Acrosome Reaction Animals Cell Membrane Epididymis Female Galectin 1 Gene Expression Immunoblotting Male Membrane Potentials Mice, Inbred C57BL Mice, Knockout Polysaccharides Progesterone Recombinant Proteins Reverse Transcriptase Polymerase Chain Reaction Sperm Capacitation Sperm Motility Spermatozoa Testis
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08926638_v29_n10_p4189_Vasen http://hdl.handle.net/20.500.12110/paper_08926638_v29_n10_p4189_Vasen
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_08926638_v29_n10_p4189_Vasen
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spelling	paper:paper_08926638_v29_n10_p4189_Vasen2023-06-08T15:47:23Z The galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization Croci Russo, Diego Omar Brukman, Nicolás G. Cuasnicú, Patricia S. Acrosome reaction Capacitation Fertilization Glycosylation galectin 1 glycan progesterone galectin 1 polysaccharide progesterone recombinant protein acrosome reaction adult animal cell animal tissue Article cell volume controlled study cumulus cell enzyme linked immunosorbent assay epididymis female fertilization fertilization in vitro flow cytometry gamete genotype hyperpolarization immunoblotting in vitro study in vivo study male membrane fusion membrane hyperpolarization membrane potential mouse nonhuman oocyte ovary oviduct priority journal protein expression protein phosphorylation spermatozoon spermatozoon capacitation spermatozoon motility testis uterus young adult zygote animal C57BL mouse cell membrane cytology drug effects fertilization gene expression genetics knockout mouse metabolism physiology reverse transcription polymerase chain reaction spermatozoon capacitation spermatozoon motility Acrosome Reaction Animals Cell Membrane Epididymis Female Fertilization Galectin 1 Gene Expression Immunoblotting Male Membrane Potentials Mice, Inbred C57BL Mice, Knockout Polysaccharides Progesterone Recombinant Proteins Reverse Transcriptase Polymerase Chain Reaction Sperm Capacitation Sperm Motility Spermatozoa Testis Lectin-glycan recognition systems play central roles in many physiologic and pathologic processes. We identified a role for galectin-1 (Gal-1), a highly conserved glycan-binding protein, in the control of sperm function. We found that Gal-1 is expressed in the epididymis and associates with sperm during epididymal maturation. Exposure of sperm to Gal-1 resulted in glycandependent modulation of the acrosome reaction (AR), a key event in the fertilization process. Gal-1-deficient (Lgals12/2 ) mice revealed the essential contribution of this lectin for full sperm fertilizing ability both in vitro and in vivo. Mechanistically, Lgals12/2 sperm exhibited defects in their ability to develop hyperactivation, a vigorous motility required for penetration of the egg vestments. Moreover, Lgals12/2 sperm showed a decreased ability to control cell volume and to undergo progesterone-induced AR, phenotypes that were rescued by exposure of the cells to recombinant Gal-1. Interestingly, the AR defect was associated with a deficiency in sperm membrane potential hyperpolarization. Our study highlights the relevance of the Gal-1-glycan axis in sperm function with critical implications in mammalian reproductive biology.-Vasen, G., Battistone, M. A., Croci, D. O., Brukman, N. G., Weigel Muńoz, M., Stupirski, J. C., Rabinovich, G. A., Cuasnicú, P. S. The galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization. © FASEB. Fil:Croci, D.O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Brukman, N.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Cuasnicú, P.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08926638_v29_n10_p4189_Vasen http://hdl.handle.net/20.500.12110/paper_08926638_v29_n10_p4189_Vasen
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Acrosome reaction Capacitation Fertilization Glycosylation galectin 1 glycan progesterone galectin 1 polysaccharide progesterone recombinant protein acrosome reaction adult animal cell animal tissue Article cell volume controlled study cumulus cell enzyme linked immunosorbent assay epididymis female fertilization fertilization in vitro flow cytometry gamete genotype hyperpolarization immunoblotting in vitro study in vivo study male membrane fusion membrane hyperpolarization membrane potential mouse nonhuman oocyte ovary oviduct priority journal protein expression protein phosphorylation spermatozoon spermatozoon capacitation spermatozoon motility testis uterus young adult zygote animal C57BL mouse cell membrane cytology drug effects fertilization gene expression genetics knockout mouse metabolism physiology reverse transcription polymerase chain reaction spermatozoon capacitation spermatozoon motility Acrosome Reaction Animals Cell Membrane Epididymis Female Fertilization Galectin 1 Gene Expression Immunoblotting Male Membrane Potentials Mice, Inbred C57BL Mice, Knockout Polysaccharides Progesterone Recombinant Proteins Reverse Transcriptase Polymerase Chain Reaction Sperm Capacitation Sperm Motility Spermatozoa Testis
spellingShingle	Acrosome reaction Capacitation Fertilization Glycosylation galectin 1 glycan progesterone galectin 1 polysaccharide progesterone recombinant protein acrosome reaction adult animal cell animal tissue Article cell volume controlled study cumulus cell enzyme linked immunosorbent assay epididymis female fertilization fertilization in vitro flow cytometry gamete genotype hyperpolarization immunoblotting in vitro study in vivo study male membrane fusion membrane hyperpolarization membrane potential mouse nonhuman oocyte ovary oviduct priority journal protein expression protein phosphorylation spermatozoon spermatozoon capacitation spermatozoon motility testis uterus young adult zygote animal C57BL mouse cell membrane cytology drug effects fertilization gene expression genetics knockout mouse metabolism physiology reverse transcription polymerase chain reaction spermatozoon capacitation spermatozoon motility Acrosome Reaction Animals Cell Membrane Epididymis Female Fertilization Galectin 1 Gene Expression Immunoblotting Male Membrane Potentials Mice, Inbred C57BL Mice, Knockout Polysaccharides Progesterone Recombinant Proteins Reverse Transcriptase Polymerase Chain Reaction Sperm Capacitation Sperm Motility Spermatozoa Testis Croci Russo, Diego Omar Brukman, Nicolás G. Cuasnicú, Patricia S. The galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization
topic_facet	Acrosome reaction Capacitation Fertilization Glycosylation galectin 1 glycan progesterone galectin 1 polysaccharide progesterone recombinant protein acrosome reaction adult animal cell animal tissue Article cell volume controlled study cumulus cell enzyme linked immunosorbent assay epididymis female fertilization fertilization in vitro flow cytometry gamete genotype hyperpolarization immunoblotting in vitro study in vivo study male membrane fusion membrane hyperpolarization membrane potential mouse nonhuman oocyte ovary oviduct priority journal protein expression protein phosphorylation spermatozoon spermatozoon capacitation spermatozoon motility testis uterus young adult zygote animal C57BL mouse cell membrane cytology drug effects fertilization gene expression genetics knockout mouse metabolism physiology reverse transcription polymerase chain reaction spermatozoon capacitation spermatozoon motility Acrosome Reaction Animals Cell Membrane Epididymis Female Fertilization Galectin 1 Gene Expression Immunoblotting Male Membrane Potentials Mice, Inbred C57BL Mice, Knockout Polysaccharides Progesterone Recombinant Proteins Reverse Transcriptase Polymerase Chain Reaction Sperm Capacitation Sperm Motility Spermatozoa Testis
description	Lectin-glycan recognition systems play central roles in many physiologic and pathologic processes. We identified a role for galectin-1 (Gal-1), a highly conserved glycan-binding protein, in the control of sperm function. We found that Gal-1 is expressed in the epididymis and associates with sperm during epididymal maturation. Exposure of sperm to Gal-1 resulted in glycandependent modulation of the acrosome reaction (AR), a key event in the fertilization process. Gal-1-deficient (Lgals12/2 ) mice revealed the essential contribution of this lectin for full sperm fertilizing ability both in vitro and in vivo. Mechanistically, Lgals12/2 sperm exhibited defects in their ability to develop hyperactivation, a vigorous motility required for penetration of the egg vestments. Moreover, Lgals12/2 sperm showed a decreased ability to control cell volume and to undergo progesterone-induced AR, phenotypes that were rescued by exposure of the cells to recombinant Gal-1. Interestingly, the AR defect was associated with a deficiency in sperm membrane potential hyperpolarization. Our study highlights the relevance of the Gal-1-glycan axis in sperm function with critical implications in mammalian reproductive biology.-Vasen, G., Battistone, M. A., Croci, D. O., Brukman, N. G., Weigel Muńoz, M., Stupirski, J. C., Rabinovich, G. A., Cuasnicú, P. S. The galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization. © FASEB.
author	Croci Russo, Diego Omar Brukman, Nicolás G. Cuasnicú, Patricia S.
author_facet	Croci Russo, Diego Omar Brukman, Nicolás G. Cuasnicú, Patricia S.
author_sort	Croci Russo, Diego Omar
title	The galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization
title_short	The galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization
title_full	The galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization
title_fullStr	The galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization
title_full_unstemmed	The galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization
title_sort	galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization
publishDate	2015
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08926638_v29_n10_p4189_Vasen http://hdl.handle.net/20.500.12110/paper_08926638_v29_n10_p4189_Vasen
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The galectin-1-glycan axis controls sperm fertilizing capacity by regulating sperm motility and membrane hyperpolarization

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