Genomic affinities in Turnera (subseries Turnera, Turneraceae) inferred by in situ hybridization techniques
Subseries Turnera comprises a polyploid complex with ploidy levels ranging from diploid (2n = 2x = 10) to octoploid (2n = 8x = 40). The use of fluorescent in situ hybridization greatly improved the knowledge of the karyotypes of Turnera species by detecting and mapping rDNA sites. Interspecific vari...
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2010
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v53_n8_p594_Lopez http://hdl.handle.net/20.500.12110/paper_08312796_v53_n8_p594_Lopez |
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paper:paper_08312796_v53_n8_p594_Lopez2023-06-08T15:46:17Z Genomic affinities in Turnera (subseries Turnera, Turneraceae) inferred by in situ hybridization techniques López, Alicia Poggio, Lidia Gish-fish In situ hybridization Turnera ribosome DNA article chimera chromosome map classification DNA probe enzyme specificity fluorescence in situ hybridization genetics genomics metabolism methodology plant genome species difference Turnera Chimera Chromosome Mapping DNA Probes DNA, Ribosomal Genome, Plant Genomics In Situ Hybridization, Fluorescence Species Specificity Substrate Specificity Turnera Turnera Turneraceae Subseries Turnera comprises a polyploid complex with ploidy levels ranging from diploid (2n = 2x = 10) to octoploid (2n = 8x = 40). The use of fluorescent in situ hybridization greatly improved the knowledge of the karyotypes of Turnera species by detecting and mapping rDNA sites. Interspecific variability in the number of sites was detected, but not in correlation with the ploidy level. A chromosome pair with a strong hybridization signal was always visible and this signal corresponded to the secondary constriction detectable by conventional techniques. Genomic in situ hybridization experiments combined with information on meiotic pairing in species and interspecific hybrids revealed that homologies detected by molecular analysis are greater than those detected by chromosome pairing. This suggests that the formation of the allopolyploids could involve species more closely related than previously assumed. Despite the molecular affinity among the genomes, the meiotic pairing is probably controlled by specific genes that restrict homeologous pairing in polyploids. Fil:López, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Poggio, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2010 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v53_n8_p594_Lopez http://hdl.handle.net/20.500.12110/paper_08312796_v53_n8_p594_Lopez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Gish-fish In situ hybridization Turnera ribosome DNA article chimera chromosome map classification DNA probe enzyme specificity fluorescence in situ hybridization genetics genomics metabolism methodology plant genome species difference Turnera Chimera Chromosome Mapping DNA Probes DNA, Ribosomal Genome, Plant Genomics In Situ Hybridization, Fluorescence Species Specificity Substrate Specificity Turnera Turnera Turneraceae |
| spellingShingle |
Gish-fish In situ hybridization Turnera ribosome DNA article chimera chromosome map classification DNA probe enzyme specificity fluorescence in situ hybridization genetics genomics metabolism methodology plant genome species difference Turnera Chimera Chromosome Mapping DNA Probes DNA, Ribosomal Genome, Plant Genomics In Situ Hybridization, Fluorescence Species Specificity Substrate Specificity Turnera Turnera Turneraceae López, Alicia Poggio, Lidia Genomic affinities in Turnera (subseries Turnera, Turneraceae) inferred by in situ hybridization techniques |
| topic_facet |
Gish-fish In situ hybridization Turnera ribosome DNA article chimera chromosome map classification DNA probe enzyme specificity fluorescence in situ hybridization genetics genomics metabolism methodology plant genome species difference Turnera Chimera Chromosome Mapping DNA Probes DNA, Ribosomal Genome, Plant Genomics In Situ Hybridization, Fluorescence Species Specificity Substrate Specificity Turnera Turnera Turneraceae |
| description |
Subseries Turnera comprises a polyploid complex with ploidy levels ranging from diploid (2n = 2x = 10) to octoploid (2n = 8x = 40). The use of fluorescent in situ hybridization greatly improved the knowledge of the karyotypes of Turnera species by detecting and mapping rDNA sites. Interspecific variability in the number of sites was detected, but not in correlation with the ploidy level. A chromosome pair with a strong hybridization signal was always visible and this signal corresponded to the secondary constriction detectable by conventional techniques. Genomic in situ hybridization experiments combined with information on meiotic pairing in species and interspecific hybrids revealed that homologies detected by molecular analysis are greater than those detected by chromosome pairing. This suggests that the formation of the allopolyploids could involve species more closely related than previously assumed. Despite the molecular affinity among the genomes, the meiotic pairing is probably controlled by specific genes that restrict homeologous pairing in polyploids. |
| author |
López, Alicia Poggio, Lidia |
| author_facet |
López, Alicia Poggio, Lidia |
| author_sort |
López, Alicia |
| title |
Genomic affinities in Turnera (subseries Turnera, Turneraceae) inferred by in situ hybridization techniques |
| title_short |
Genomic affinities in Turnera (subseries Turnera, Turneraceae) inferred by in situ hybridization techniques |
| title_full |
Genomic affinities in Turnera (subseries Turnera, Turneraceae) inferred by in situ hybridization techniques |
| title_fullStr |
Genomic affinities in Turnera (subseries Turnera, Turneraceae) inferred by in situ hybridization techniques |
| title_full_unstemmed |
Genomic affinities in Turnera (subseries Turnera, Turneraceae) inferred by in situ hybridization techniques |
| title_sort |
genomic affinities in turnera (subseries turnera, turneraceae) inferred by in situ hybridization techniques |
| publishDate |
2010 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v53_n8_p594_Lopez http://hdl.handle.net/20.500.12110/paper_08312796_v53_n8_p594_Lopez |
| work_keys_str_mv |
AT lopezalicia genomicaffinitiesinturnerasubseriesturneraturneraceaeinferredbyinsituhybridizationtechniques AT poggiolidia genomicaffinitiesinturnerasubseriesturneraturneraceaeinferredbyinsituhybridizationtechniques |
| _version_ |
1768543089719246848 |