Mitochondrial DNA variation in the grasshopper Sinipta dalmani: Application of long-PCR to the development of a homologous probe
RFLP analysis of mtDNA in natural populations is a valuable tool for phylogeographic and population genetic studies. The amplification of long DNA fragments using universal primers may contribute to the development of novel homologous probes in species for which no previous genomic information is av...
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2005
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v48_n6_p971_Pensel http://hdl.handle.net/20.500.12110/paper_08312796_v48_n6_p971_Pensel |
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paper:paper_08312796_v48_n6_p971_Pensel2023-06-08T15:46:17Z Mitochondrial DNA variation in the grasshopper Sinipta dalmani: Application of long-PCR to the development of a homologous probe Pensel, Sara M. Vilardi, Juan Cesar Remis, Maria Isabel Grasshopper Long-PCR Mitochondrial DNA RFLP Universal primers mitochondrial DNA animal article DNA probe genetic variability genetics grasshopper mitochondrion nucleotide sequence polymerase chain reaction restriction fragment length polymorphism sequence homology Animals Conserved Sequence DNA Probes DNA, Mitochondrial Genetic Variation Grasshoppers Mitochondria Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Sequence Homology, Nucleic Acid Variation (Genetics) Orthoptera Sinipta dalmani RFLP analysis of mtDNA in natural populations is a valuable tool for phylogeographic and population genetic studies. The amplification of long DNA fragments using universal primers may contribute to the development of novel homologous probes in species for which no previous genomic information is available. Here we report how we obtained the complete mtDNA genome of Sinipta dalmani (Orthoptera) in 2 fragments (7 and 9 kb) using primers of conserved regions. The specificity of the PCR reactions was ultimately confirmed by several lines of evidence. These fragments were used as a probe for a mtDNA RFLP study in S. dalmani that analyzed the pattern of haplotype distribution and nucleotide diversity within and among chromosomally differentiated natural populations. Our results suggest that the restriction in gene flow detected at the molecular level may explain the chromosome differentiation detected previously and the maintenance of chromosome polymorphism in some areas of S. dalmani geographic distribution. © 2005 NRC. Fil:Pensel, S.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Vilardi, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Remis, M.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2005 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v48_n6_p971_Pensel http://hdl.handle.net/20.500.12110/paper_08312796_v48_n6_p971_Pensel |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Grasshopper Long-PCR Mitochondrial DNA RFLP Universal primers mitochondrial DNA animal article DNA probe genetic variability genetics grasshopper mitochondrion nucleotide sequence polymerase chain reaction restriction fragment length polymorphism sequence homology Animals Conserved Sequence DNA Probes DNA, Mitochondrial Genetic Variation Grasshoppers Mitochondria Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Sequence Homology, Nucleic Acid Variation (Genetics) Orthoptera Sinipta dalmani |
| spellingShingle |
Grasshopper Long-PCR Mitochondrial DNA RFLP Universal primers mitochondrial DNA animal article DNA probe genetic variability genetics grasshopper mitochondrion nucleotide sequence polymerase chain reaction restriction fragment length polymorphism sequence homology Animals Conserved Sequence DNA Probes DNA, Mitochondrial Genetic Variation Grasshoppers Mitochondria Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Sequence Homology, Nucleic Acid Variation (Genetics) Orthoptera Sinipta dalmani Pensel, Sara M. Vilardi, Juan Cesar Remis, Maria Isabel Mitochondrial DNA variation in the grasshopper Sinipta dalmani: Application of long-PCR to the development of a homologous probe |
| topic_facet |
Grasshopper Long-PCR Mitochondrial DNA RFLP Universal primers mitochondrial DNA animal article DNA probe genetic variability genetics grasshopper mitochondrion nucleotide sequence polymerase chain reaction restriction fragment length polymorphism sequence homology Animals Conserved Sequence DNA Probes DNA, Mitochondrial Genetic Variation Grasshoppers Mitochondria Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Sequence Homology, Nucleic Acid Variation (Genetics) Orthoptera Sinipta dalmani |
| description |
RFLP analysis of mtDNA in natural populations is a valuable tool for phylogeographic and population genetic studies. The amplification of long DNA fragments using universal primers may contribute to the development of novel homologous probes in species for which no previous genomic information is available. Here we report how we obtained the complete mtDNA genome of Sinipta dalmani (Orthoptera) in 2 fragments (7 and 9 kb) using primers of conserved regions. The specificity of the PCR reactions was ultimately confirmed by several lines of evidence. These fragments were used as a probe for a mtDNA RFLP study in S. dalmani that analyzed the pattern of haplotype distribution and nucleotide diversity within and among chromosomally differentiated natural populations. Our results suggest that the restriction in gene flow detected at the molecular level may explain the chromosome differentiation detected previously and the maintenance of chromosome polymorphism in some areas of S. dalmani geographic distribution. © 2005 NRC. |
| author |
Pensel, Sara M. Vilardi, Juan Cesar Remis, Maria Isabel |
| author_facet |
Pensel, Sara M. Vilardi, Juan Cesar Remis, Maria Isabel |
| author_sort |
Pensel, Sara M. |
| title |
Mitochondrial DNA variation in the grasshopper Sinipta dalmani: Application of long-PCR to the development of a homologous probe |
| title_short |
Mitochondrial DNA variation in the grasshopper Sinipta dalmani: Application of long-PCR to the development of a homologous probe |
| title_full |
Mitochondrial DNA variation in the grasshopper Sinipta dalmani: Application of long-PCR to the development of a homologous probe |
| title_fullStr |
Mitochondrial DNA variation in the grasshopper Sinipta dalmani: Application of long-PCR to the development of a homologous probe |
| title_full_unstemmed |
Mitochondrial DNA variation in the grasshopper Sinipta dalmani: Application of long-PCR to the development of a homologous probe |
| title_sort |
mitochondrial dna variation in the grasshopper sinipta dalmani: application of long-pcr to the development of a homologous probe |
| publishDate |
2005 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08312796_v48_n6_p971_Pensel http://hdl.handle.net/20.500.12110/paper_08312796_v48_n6_p971_Pensel |
| work_keys_str_mv |
AT penselsaram mitochondrialdnavariationinthegrasshoppersiniptadalmaniapplicationoflongpcrtothedevelopmentofahomologousprobe AT vilardijuancesar mitochondrialdnavariationinthegrasshoppersiniptadalmaniapplicationoflongpcrtothedevelopmentofahomologousprobe AT remismariaisabel mitochondrialdnavariationinthegrasshoppersiniptadalmaniapplicationoflongpcrtothedevelopmentofahomologousprobe |
| _version_ |
1768543661687046144 |