Pleiotropic effects of 5-aminolevulinic acid in mouse brain
5-Aminolevulinic acid (ALA) seems to be responsible for the neuropsychiatric manifestations of acute intermittent porphyria (AIP). Our aim was to study the effect of ALA on the different metabolic pathways in the mouse brain to enhance our knowledge about the action of this heme precursor on the cen...
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08298211_v94_n4_p297_Lavandera http://hdl.handle.net/20.500.12110/paper_08298211_v94_n4_p297_Lavandera |
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paper:paper_08298211_v94_n4_p297_Lavandera2023-06-08T15:46:13Z Pleiotropic effects of 5-aminolevulinic acid in mouse brain Lavandera, Jimena Verónica Rodríguez, Jorge Andrés Martínez, María del Carmen Gerez, Esther Noemí Batlle, Alcira María del Carmen Buzaleh, Ana María 5-aminolevulinic acid Antioxidant defense system Cholinergic system Heme metabolism Nitric oxide synthase Antioxidants Enzymes Metabolism Nitric oxide Oxygen Physiology Porphyrins 5-aminolevulinic acid Anti-oxidant response Antioxidant defense system Central nervous systems Cholinergic systems Nitric-oxide synthase Pleiotropic effects Superoxide dismutase activities Mammals aminolevulinic acid cholinesterase heme oxygenase malonaldehyde nitric oxide synthase superoxide dismutase acetylcholinesterase aminolevulinic acid antioxidant heme nitric oxide synthase photosensitizing agent acute intermittent porphyria animal experiment animal model animal tissue Article brain brain homogenate brain metabolism cholinergic system controlled study enzyme activity glia cell immunohistochemistry long term care male morning dosage mouse nonhuman oxidative stress pleiotropy single drug dose treatment duration Western blotting animal brain drug effects metabolism pathology Acetylcholinesterase Aminolevulinic Acid Animals Antioxidants Brain Heme Male Mice Nitric Oxide Synthase Oxidative Stress Photosensitizing Agents 5-Aminolevulinic acid (ALA) seems to be responsible for the neuropsychiatric manifestations of acute intermittent porphyria (AIP). Our aim was to study the effect of ALA on the different metabolic pathways in the mouse brain to enhance our knowledge about the action of this heme precursor on the central nervous system. Heme metabolism, the cholinergic system, the defense enzyme system, and nitric oxide metabolism were evaluated in the encephalon of CF-1 mice receiving a single (40 mg/kg body mass) or multiple doses of ALA (40 mg/kg, every 48 h for 14 days). We subsequently found ALA accumulation in the encephalon of the mice. ALA also altered the brain cholinergic system. After one dose of ALA, a decrease in superoxide dismutase activity and a reduction in glutathione levels were detected, whereas malondialdehyde levels and catalase activity were increased. Heme oxygenase was also increased as an antioxidant response to protect the encephalon against injury. All nitric oxide synthase isoforms were induced by ALA, these changes were more significant for the inducible isoform in glial cells. In conclusion, ALA affected several metabolic pathways in mouse encephalon. Data indicate that a rapid response to oxidative stress was developed; however, with long-term intoxication, the redox balance was probably restored, thereby minimizing oxidative damage. © 2016 Published by NRC Research Press. Fil:Lavandera, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodríguez, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Martínez, M.D.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Gerez, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Buzaleh, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2016 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08298211_v94_n4_p297_Lavandera http://hdl.handle.net/20.500.12110/paper_08298211_v94_n4_p297_Lavandera |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
5-aminolevulinic acid Antioxidant defense system Cholinergic system Heme metabolism Nitric oxide synthase Antioxidants Enzymes Metabolism Nitric oxide Oxygen Physiology Porphyrins 5-aminolevulinic acid Anti-oxidant response Antioxidant defense system Central nervous systems Cholinergic systems Nitric-oxide synthase Pleiotropic effects Superoxide dismutase activities Mammals aminolevulinic acid cholinesterase heme oxygenase malonaldehyde nitric oxide synthase superoxide dismutase acetylcholinesterase aminolevulinic acid antioxidant heme nitric oxide synthase photosensitizing agent acute intermittent porphyria animal experiment animal model animal tissue Article brain brain homogenate brain metabolism cholinergic system controlled study enzyme activity glia cell immunohistochemistry long term care male morning dosage mouse nonhuman oxidative stress pleiotropy single drug dose treatment duration Western blotting animal brain drug effects metabolism pathology Acetylcholinesterase Aminolevulinic Acid Animals Antioxidants Brain Heme Male Mice Nitric Oxide Synthase Oxidative Stress Photosensitizing Agents |
spellingShingle |
5-aminolevulinic acid Antioxidant defense system Cholinergic system Heme metabolism Nitric oxide synthase Antioxidants Enzymes Metabolism Nitric oxide Oxygen Physiology Porphyrins 5-aminolevulinic acid Anti-oxidant response Antioxidant defense system Central nervous systems Cholinergic systems Nitric-oxide synthase Pleiotropic effects Superoxide dismutase activities Mammals aminolevulinic acid cholinesterase heme oxygenase malonaldehyde nitric oxide synthase superoxide dismutase acetylcholinesterase aminolevulinic acid antioxidant heme nitric oxide synthase photosensitizing agent acute intermittent porphyria animal experiment animal model animal tissue Article brain brain homogenate brain metabolism cholinergic system controlled study enzyme activity glia cell immunohistochemistry long term care male morning dosage mouse nonhuman oxidative stress pleiotropy single drug dose treatment duration Western blotting animal brain drug effects metabolism pathology Acetylcholinesterase Aminolevulinic Acid Animals Antioxidants Brain Heme Male Mice Nitric Oxide Synthase Oxidative Stress Photosensitizing Agents Lavandera, Jimena Verónica Rodríguez, Jorge Andrés Martínez, María del Carmen Gerez, Esther Noemí Batlle, Alcira María del Carmen Buzaleh, Ana María Pleiotropic effects of 5-aminolevulinic acid in mouse brain |
topic_facet |
5-aminolevulinic acid Antioxidant defense system Cholinergic system Heme metabolism Nitric oxide synthase Antioxidants Enzymes Metabolism Nitric oxide Oxygen Physiology Porphyrins 5-aminolevulinic acid Anti-oxidant response Antioxidant defense system Central nervous systems Cholinergic systems Nitric-oxide synthase Pleiotropic effects Superoxide dismutase activities Mammals aminolevulinic acid cholinesterase heme oxygenase malonaldehyde nitric oxide synthase superoxide dismutase acetylcholinesterase aminolevulinic acid antioxidant heme nitric oxide synthase photosensitizing agent acute intermittent porphyria animal experiment animal model animal tissue Article brain brain homogenate brain metabolism cholinergic system controlled study enzyme activity glia cell immunohistochemistry long term care male morning dosage mouse nonhuman oxidative stress pleiotropy single drug dose treatment duration Western blotting animal brain drug effects metabolism pathology Acetylcholinesterase Aminolevulinic Acid Animals Antioxidants Brain Heme Male Mice Nitric Oxide Synthase Oxidative Stress Photosensitizing Agents |
description |
5-Aminolevulinic acid (ALA) seems to be responsible for the neuropsychiatric manifestations of acute intermittent porphyria (AIP). Our aim was to study the effect of ALA on the different metabolic pathways in the mouse brain to enhance our knowledge about the action of this heme precursor on the central nervous system. Heme metabolism, the cholinergic system, the defense enzyme system, and nitric oxide metabolism were evaluated in the encephalon of CF-1 mice receiving a single (40 mg/kg body mass) or multiple doses of ALA (40 mg/kg, every 48 h for 14 days). We subsequently found ALA accumulation in the encephalon of the mice. ALA also altered the brain cholinergic system. After one dose of ALA, a decrease in superoxide dismutase activity and a reduction in glutathione levels were detected, whereas malondialdehyde levels and catalase activity were increased. Heme oxygenase was also increased as an antioxidant response to protect the encephalon against injury. All nitric oxide synthase isoforms were induced by ALA, these changes were more significant for the inducible isoform in glial cells. In conclusion, ALA affected several metabolic pathways in mouse encephalon. Data indicate that a rapid response to oxidative stress was developed; however, with long-term intoxication, the redox balance was probably restored, thereby minimizing oxidative damage. © 2016 Published by NRC Research Press. |
author |
Lavandera, Jimena Verónica Rodríguez, Jorge Andrés Martínez, María del Carmen Gerez, Esther Noemí Batlle, Alcira María del Carmen Buzaleh, Ana María |
author_facet |
Lavandera, Jimena Verónica Rodríguez, Jorge Andrés Martínez, María del Carmen Gerez, Esther Noemí Batlle, Alcira María del Carmen Buzaleh, Ana María |
author_sort |
Lavandera, Jimena Verónica |
title |
Pleiotropic effects of 5-aminolevulinic acid in mouse brain |
title_short |
Pleiotropic effects of 5-aminolevulinic acid in mouse brain |
title_full |
Pleiotropic effects of 5-aminolevulinic acid in mouse brain |
title_fullStr |
Pleiotropic effects of 5-aminolevulinic acid in mouse brain |
title_full_unstemmed |
Pleiotropic effects of 5-aminolevulinic acid in mouse brain |
title_sort |
pleiotropic effects of 5-aminolevulinic acid in mouse brain |
publishDate |
2016 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08298211_v94_n4_p297_Lavandera http://hdl.handle.net/20.500.12110/paper_08298211_v94_n4_p297_Lavandera |
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