Cytochrome P450 expression in mouse brain: Specific isoenzymes involved in Phase i metabolizing system of porphyrinogenic agents in both microsomes and mitochondria

Brain cytochrome P450 (CYP) metabolizes a variety of drugs to produce their pharmacological effects within the brain. We have previously observed that porphyrinogenic agents altered CYP levels in brain. The aim of this work was to further study the involvement of mice brain mitochondrial and microso...

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Autores principales:	Lavandera, Jimena Verónica, Batlle, Alcira María del Carmen, Buzaleh, Ana María
Publicado:	2015
Materias:	Brain Cytochrome P450 Porphyrinogenic agents Volatile anaesthetics Anesthetics Enzymes Ethanol Mammals Cytochrome p450 Microsomal proteins Mitochondrial activity NADPH-cytochrome P450 reductase Neurological disorders Pharmacological effects Western blots Whole brains Mitochondria alcohol allylisopropylacetamide barbital cytochrome P450 2B1 cytochrome P450 2E1 cytochrome P450 3A4 drug metabolizing enzyme enflurane griseofulvin isoenzyme isoflurane porphyrinogen reduced nicotinamide adenine dinucleotide ferrihemoprotein reductase cytochrome P450 cytochrome P450 3A adult animal experiment Article brain brain microsome brain mitochondrion cell fractionation controlled study immunoblotting male mouse nonhuman protein expression Western blotting animal drug effects enzymology metabolism microsome mitochondrion Animalia Mus Animals Cytochrome P-450 CYP2B1 Cytochrome P-450 CYP2E1 Cytochrome P-450 CYP3A Cytochrome P-450 Enzyme System Isoenzymes Male Mice Microsomes
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08298211_v93_n1_p102_Lavandera http://hdl.handle.net/20.500.12110/paper_08298211_v93_n1_p102_Lavandera
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_08298211_v93_n1_p102_Lavandera
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spelling	paper:paper_08298211_v93_n1_p102_Lavandera2023-06-08T15:46:13Z Cytochrome P450 expression in mouse brain: Specific isoenzymes involved in Phase i metabolizing system of porphyrinogenic agents in both microsomes and mitochondria Lavandera, Jimena Verónica Batlle, Alcira María del Carmen Buzaleh, Ana María Brain Cytochrome P450 Porphyrinogenic agents Volatile anaesthetics Anesthetics Brain Enzymes Ethanol Mammals Cytochrome p450 Microsomal proteins Mitochondrial activity NADPH-cytochrome P450 reductase Neurological disorders Pharmacological effects Western blots Whole brains Mitochondria alcohol allylisopropylacetamide barbital cytochrome P450 2B1 cytochrome P450 2E1 cytochrome P450 3A4 drug metabolizing enzyme enflurane griseofulvin isoenzyme isoflurane porphyrinogen reduced nicotinamide adenine dinucleotide ferrihemoprotein reductase cytochrome P450 cytochrome P450 2B1 cytochrome P450 2E1 cytochrome P450 3A isoenzyme adult animal experiment Article brain brain microsome brain mitochondrion cell fractionation controlled study immunoblotting male mouse nonhuman protein expression Western blotting animal brain drug effects enzymology metabolism microsome mitochondrion Animalia Mus Animals Brain Cytochrome P-450 CYP2B1 Cytochrome P-450 CYP2E1 Cytochrome P-450 CYP3A Cytochrome P-450 Enzyme System Isoenzymes Male Mice Microsomes Mitochondria Brain cytochrome P450 (CYP) metabolizes a variety of drugs to produce their pharmacological effects within the brain. We have previously observed that porphyrinogenic agents altered CYP levels in brain. The aim of this work was to further study the involvement of mice brain mitochondrial and microsomal Phase I drug metabolizing system when porphyrinogenic agents, such as Enflurane, Isoflurane, allylisopropylacetamide, veronal, ethanol, and Griseofulvin were administered. To this end, CYP2E1, CYP2B1, and CYP3A4 expression were measured. NADPH cytochrome P450 reductase (CPR) expression was also determined. Western Blots were performed in microsomes and mitochondria of whole brain. Some of the drugs studied altered expression mainly in microsomes. Chronic Isoflurane augmented mitochondrial isoform, although this anaesthetic diminished microsomal expression. Ethanol and topical Griseofulvin affected expression in microsomes but not in mitochondria. CYP2E1 mitochondrial activity was induced by acute Enflurane; while the activity of the microsomal protein was enhanced in alcoholised animals. Ethanol also induced CYP2E1 expression in microsomes, although Isoflurane provoked opposite effects in mitochondria and microsomes. Expression of CPR was also induced. Several reports support an emergent role of CYP enzymes in the pathogenesis of neurological disorders, so CYP response in brain could be one of the multiples factors influencing porphyria acute attacks. © 2015 Published by NRC Research Press. Fil:Lavandera, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Buzaleh, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08298211_v93_n1_p102_Lavandera http://hdl.handle.net/20.500.12110/paper_08298211_v93_n1_p102_Lavandera
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Brain Cytochrome P450 Porphyrinogenic agents Volatile anaesthetics Anesthetics Brain Enzymes Ethanol Mammals Cytochrome p450 Microsomal proteins Mitochondrial activity NADPH-cytochrome P450 reductase Neurological disorders Pharmacological effects Western blots Whole brains Mitochondria alcohol allylisopropylacetamide barbital cytochrome P450 2B1 cytochrome P450 2E1 cytochrome P450 3A4 drug metabolizing enzyme enflurane griseofulvin isoenzyme isoflurane porphyrinogen reduced nicotinamide adenine dinucleotide ferrihemoprotein reductase cytochrome P450 cytochrome P450 2B1 cytochrome P450 2E1 cytochrome P450 3A isoenzyme adult animal experiment Article brain brain microsome brain mitochondrion cell fractionation controlled study immunoblotting male mouse nonhuman protein expression Western blotting animal brain drug effects enzymology metabolism microsome mitochondrion Animalia Mus Animals Brain Cytochrome P-450 CYP2B1 Cytochrome P-450 CYP2E1 Cytochrome P-450 CYP3A Cytochrome P-450 Enzyme System Isoenzymes Male Mice Microsomes Mitochondria
spellingShingle	Brain Cytochrome P450 Porphyrinogenic agents Volatile anaesthetics Anesthetics Brain Enzymes Ethanol Mammals Cytochrome p450 Microsomal proteins Mitochondrial activity NADPH-cytochrome P450 reductase Neurological disorders Pharmacological effects Western blots Whole brains Mitochondria alcohol allylisopropylacetamide barbital cytochrome P450 2B1 cytochrome P450 2E1 cytochrome P450 3A4 drug metabolizing enzyme enflurane griseofulvin isoenzyme isoflurane porphyrinogen reduced nicotinamide adenine dinucleotide ferrihemoprotein reductase cytochrome P450 cytochrome P450 2B1 cytochrome P450 2E1 cytochrome P450 3A isoenzyme adult animal experiment Article brain brain microsome brain mitochondrion cell fractionation controlled study immunoblotting male mouse nonhuman protein expression Western blotting animal brain drug effects enzymology metabolism microsome mitochondrion Animalia Mus Animals Brain Cytochrome P-450 CYP2B1 Cytochrome P-450 CYP2E1 Cytochrome P-450 CYP3A Cytochrome P-450 Enzyme System Isoenzymes Male Mice Microsomes Mitochondria Lavandera, Jimena Verónica Batlle, Alcira María del Carmen Buzaleh, Ana María Cytochrome P450 expression in mouse brain: Specific isoenzymes involved in Phase i metabolizing system of porphyrinogenic agents in both microsomes and mitochondria
topic_facet	Brain Cytochrome P450 Porphyrinogenic agents Volatile anaesthetics Anesthetics Brain Enzymes Ethanol Mammals Cytochrome p450 Microsomal proteins Mitochondrial activity NADPH-cytochrome P450 reductase Neurological disorders Pharmacological effects Western blots Whole brains Mitochondria alcohol allylisopropylacetamide barbital cytochrome P450 2B1 cytochrome P450 2E1 cytochrome P450 3A4 drug metabolizing enzyme enflurane griseofulvin isoenzyme isoflurane porphyrinogen reduced nicotinamide adenine dinucleotide ferrihemoprotein reductase cytochrome P450 cytochrome P450 2B1 cytochrome P450 2E1 cytochrome P450 3A isoenzyme adult animal experiment Article brain brain microsome brain mitochondrion cell fractionation controlled study immunoblotting male mouse nonhuman protein expression Western blotting animal brain drug effects enzymology metabolism microsome mitochondrion Animalia Mus Animals Brain Cytochrome P-450 CYP2B1 Cytochrome P-450 CYP2E1 Cytochrome P-450 CYP3A Cytochrome P-450 Enzyme System Isoenzymes Male Mice Microsomes Mitochondria
description	Brain cytochrome P450 (CYP) metabolizes a variety of drugs to produce their pharmacological effects within the brain. We have previously observed that porphyrinogenic agents altered CYP levels in brain. The aim of this work was to further study the involvement of mice brain mitochondrial and microsomal Phase I drug metabolizing system when porphyrinogenic agents, such as Enflurane, Isoflurane, allylisopropylacetamide, veronal, ethanol, and Griseofulvin were administered. To this end, CYP2E1, CYP2B1, and CYP3A4 expression were measured. NADPH cytochrome P450 reductase (CPR) expression was also determined. Western Blots were performed in microsomes and mitochondria of whole brain. Some of the drugs studied altered expression mainly in microsomes. Chronic Isoflurane augmented mitochondrial isoform, although this anaesthetic diminished microsomal expression. Ethanol and topical Griseofulvin affected expression in microsomes but not in mitochondria. CYP2E1 mitochondrial activity was induced by acute Enflurane; while the activity of the microsomal protein was enhanced in alcoholised animals. Ethanol also induced CYP2E1 expression in microsomes, although Isoflurane provoked opposite effects in mitochondria and microsomes. Expression of CPR was also induced. Several reports support an emergent role of CYP enzymes in the pathogenesis of neurological disorders, so CYP response in brain could be one of the multiples factors influencing porphyria acute attacks. © 2015 Published by NRC Research Press.
author	Lavandera, Jimena Verónica Batlle, Alcira María del Carmen Buzaleh, Ana María
author_facet	Lavandera, Jimena Verónica Batlle, Alcira María del Carmen Buzaleh, Ana María
author_sort	Lavandera, Jimena Verónica
title	Cytochrome P450 expression in mouse brain: Specific isoenzymes involved in Phase i metabolizing system of porphyrinogenic agents in both microsomes and mitochondria
title_short	Cytochrome P450 expression in mouse brain: Specific isoenzymes involved in Phase i metabolizing system of porphyrinogenic agents in both microsomes and mitochondria
title_full	Cytochrome P450 expression in mouse brain: Specific isoenzymes involved in Phase i metabolizing system of porphyrinogenic agents in both microsomes and mitochondria
title_fullStr	Cytochrome P450 expression in mouse brain: Specific isoenzymes involved in Phase i metabolizing system of porphyrinogenic agents in both microsomes and mitochondria
title_full_unstemmed	Cytochrome P450 expression in mouse brain: Specific isoenzymes involved in Phase i metabolizing system of porphyrinogenic agents in both microsomes and mitochondria
title_sort	cytochrome p450 expression in mouse brain: specific isoenzymes involved in phase i metabolizing system of porphyrinogenic agents in both microsomes and mitochondria
publishDate	2015
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08298211_v93_n1_p102_Lavandera http://hdl.handle.net/20.500.12110/paper_08298211_v93_n1_p102_Lavandera
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_version_	1768545378749120512

Cytochrome P450 expression in mouse brain: Specific isoenzymes involved in Phase i metabolizing system of porphyrinogenic agents in both microsomes and mitochondria

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