Glucose inhibits phenobarbital-induced δ-aminolevulinate synthase expression in normal but not in diabetic rat hepatocytes
In the present work, we demonstrate the presence of a glucose inhibitory effect on the phenobarbital-mediated induction of the δ-aminolevulinate synthase mRNA in normal rat hepatocytes, consistent with the results obtained with the δ-aminolevulinate synthase activity previously reported. This "...
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1996
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08298211_v74_n2_p271_Grinstein http://hdl.handle.net/20.500.12110/paper_08298211_v74_n2_p271_Grinstein |
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paper:paper_08298211_v74_n2_p271_Grinstein2023-06-08T15:46:13Z Glucose inhibits phenobarbital-induced δ-aminolevulinate synthase expression in normal but not in diabetic rat hepatocytes cAMP Diabetic rat hepatocytes Glucose Phenobarbital δ-aminolevulinate synthase expression 3',5' cyclic nucleotide phosphodiesterase 5 aminolevulinate synthase adenylate cyclase bucladesine cyclic AMP enzyme inhibitor glucose heme isobutylmethylxanthine phenobarbital streptozocin animal article biosynthesis blood chemically induced disorder drug antagonism drug effect enzyme induction enzymology experimental diabetes mellitus genetics glucose blood level innate immunity liver male metabolism physiology porphyria rat second messenger 1-Methyl-3-isobutylxanthine 3',5'-Cyclic-Nucleotide Phosphodiesterase 5-Aminolevulinate Synthetase Adenylate Cyclase Animals Blood Glucose Bucladesine Cyclic AMP Diabetes Mellitus, Experimental Enzyme Induction Enzyme Inhibitors Glucose Heme Immunity, Natural Liver Male Phenobarbital Porphyrias Rats Second Messenger Systems Streptozocin In the present work, we demonstrate the presence of a glucose inhibitory effect on the phenobarbital-mediated induction of the δ-aminolevulinate synthase mRNA in normal rat hepatocytes, consistent with the results obtained with the δ-aminolevulinate synthase activity previously reported. This "glucose effect" can be prevented by adding cAMP, adenylate cyclase activators, or a phosphodiesterase inhibitor. δ-Aminolevulinate synthase mRNA half-life is not modified in the presence of phenobarbital or glucose. When the same experiments are performed using diabetic cells, no glucose effect is observed, even when the endogenous cAMP content is lowered to normal levels. The results obtained in this study suggest that glucose decreases δ-aminolevulinate synthase biosynthesis by acting at a pretranslational step. Assuming that the glucose effect operates by a repression mechanism exerted by metabolites derived from or related to glucose, the present results may reflect a derangement in the formation of these metabolites as a result of the abnormal metabolism operating in the diabetic state. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08298211_v74_n2_p271_Grinstein http://hdl.handle.net/20.500.12110/paper_08298211_v74_n2_p271_Grinstein |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
cAMP Diabetic rat hepatocytes Glucose Phenobarbital δ-aminolevulinate synthase expression 3',5' cyclic nucleotide phosphodiesterase 5 aminolevulinate synthase adenylate cyclase bucladesine cyclic AMP enzyme inhibitor glucose heme isobutylmethylxanthine phenobarbital streptozocin animal article biosynthesis blood chemically induced disorder drug antagonism drug effect enzyme induction enzymology experimental diabetes mellitus genetics glucose blood level innate immunity liver male metabolism physiology porphyria rat second messenger 1-Methyl-3-isobutylxanthine 3',5'-Cyclic-Nucleotide Phosphodiesterase 5-Aminolevulinate Synthetase Adenylate Cyclase Animals Blood Glucose Bucladesine Cyclic AMP Diabetes Mellitus, Experimental Enzyme Induction Enzyme Inhibitors Glucose Heme Immunity, Natural Liver Male Phenobarbital Porphyrias Rats Second Messenger Systems Streptozocin |
| spellingShingle |
cAMP Diabetic rat hepatocytes Glucose Phenobarbital δ-aminolevulinate synthase expression 3',5' cyclic nucleotide phosphodiesterase 5 aminolevulinate synthase adenylate cyclase bucladesine cyclic AMP enzyme inhibitor glucose heme isobutylmethylxanthine phenobarbital streptozocin animal article biosynthesis blood chemically induced disorder drug antagonism drug effect enzyme induction enzymology experimental diabetes mellitus genetics glucose blood level innate immunity liver male metabolism physiology porphyria rat second messenger 1-Methyl-3-isobutylxanthine 3',5'-Cyclic-Nucleotide Phosphodiesterase 5-Aminolevulinate Synthetase Adenylate Cyclase Animals Blood Glucose Bucladesine Cyclic AMP Diabetes Mellitus, Experimental Enzyme Induction Enzyme Inhibitors Glucose Heme Immunity, Natural Liver Male Phenobarbital Porphyrias Rats Second Messenger Systems Streptozocin Glucose inhibits phenobarbital-induced δ-aminolevulinate synthase expression in normal but not in diabetic rat hepatocytes |
| topic_facet |
cAMP Diabetic rat hepatocytes Glucose Phenobarbital δ-aminolevulinate synthase expression 3',5' cyclic nucleotide phosphodiesterase 5 aminolevulinate synthase adenylate cyclase bucladesine cyclic AMP enzyme inhibitor glucose heme isobutylmethylxanthine phenobarbital streptozocin animal article biosynthesis blood chemically induced disorder drug antagonism drug effect enzyme induction enzymology experimental diabetes mellitus genetics glucose blood level innate immunity liver male metabolism physiology porphyria rat second messenger 1-Methyl-3-isobutylxanthine 3',5'-Cyclic-Nucleotide Phosphodiesterase 5-Aminolevulinate Synthetase Adenylate Cyclase Animals Blood Glucose Bucladesine Cyclic AMP Diabetes Mellitus, Experimental Enzyme Induction Enzyme Inhibitors Glucose Heme Immunity, Natural Liver Male Phenobarbital Porphyrias Rats Second Messenger Systems Streptozocin |
| description |
In the present work, we demonstrate the presence of a glucose inhibitory effect on the phenobarbital-mediated induction of the δ-aminolevulinate synthase mRNA in normal rat hepatocytes, consistent with the results obtained with the δ-aminolevulinate synthase activity previously reported. This "glucose effect" can be prevented by adding cAMP, adenylate cyclase activators, or a phosphodiesterase inhibitor. δ-Aminolevulinate synthase mRNA half-life is not modified in the presence of phenobarbital or glucose. When the same experiments are performed using diabetic cells, no glucose effect is observed, even when the endogenous cAMP content is lowered to normal levels. The results obtained in this study suggest that glucose decreases δ-aminolevulinate synthase biosynthesis by acting at a pretranslational step. Assuming that the glucose effect operates by a repression mechanism exerted by metabolites derived from or related to glucose, the present results may reflect a derangement in the formation of these metabolites as a result of the abnormal metabolism operating in the diabetic state. |
| title |
Glucose inhibits phenobarbital-induced δ-aminolevulinate synthase expression in normal but not in diabetic rat hepatocytes |
| title_short |
Glucose inhibits phenobarbital-induced δ-aminolevulinate synthase expression in normal but not in diabetic rat hepatocytes |
| title_full |
Glucose inhibits phenobarbital-induced δ-aminolevulinate synthase expression in normal but not in diabetic rat hepatocytes |
| title_fullStr |
Glucose inhibits phenobarbital-induced δ-aminolevulinate synthase expression in normal but not in diabetic rat hepatocytes |
| title_full_unstemmed |
Glucose inhibits phenobarbital-induced δ-aminolevulinate synthase expression in normal but not in diabetic rat hepatocytes |
| title_sort |
glucose inhibits phenobarbital-induced δ-aminolevulinate synthase expression in normal but not in diabetic rat hepatocytes |
| publishDate |
1996 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08298211_v74_n2_p271_Grinstein http://hdl.handle.net/20.500.12110/paper_08298211_v74_n2_p271_Grinstein |
| _version_ |
1768541851704360960 |