Regulation of phenobarbital-induced ferrochelatase mRNA activity by dibutyryl cAMP and glucose in normal and diabetic rat hepatocytes.
The induction of ferrochelatase activity by phenobarbital and its potentiation by dibutyryl cAMP assayed in normal rat hepatocytes are associated with increased activity of ferrochelatase mRNA. Glucose inhibits this stimulatory effect. This inhibition can be reversed with increasing concentrations o...
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1992
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08298211_v70_n1_p26_Canepa http://hdl.handle.net/20.500.12110/paper_08298211_v70_n1_p26_Canepa |
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paper:paper_08298211_v70_n1_p26_Canepa2023-06-08T15:46:12Z Regulation of phenobarbital-induced ferrochelatase mRNA activity by dibutyryl cAMP and glucose in normal and diabetic rat hepatocytes. Cánepa, Eduardo Tomás Páez Pereda, Marcelo Llambías, Elena Blanca Cecilia Grinstein, Moisés bucladesine ferrochelatase glucose messenger RNA phenobarbital animal article biosynthesis drug effect enzyme induction experimental diabetes mellitus gene expression regulation liver male metabolism rat rat strain Animals Bucladesine Diabetes Mellitus, Experimental Enzyme Induction Ferrochelatase Gene Expression Regulation Glucose Liver Male Phenobarbital Rats Rats, Inbred Strains RNA, Messenger The induction of ferrochelatase activity by phenobarbital and its potentiation by dibutyryl cAMP assayed in normal rat hepatocytes are associated with increased activity of ferrochelatase mRNA. Glucose inhibits this stimulatory effect. This inhibition can be reversed with increasing concentrations of dibutyryl cAMP. The inducing effect exerted by phenobarbital on the activity of ferrochelatase mRNA in diabetic hepatocytes is greater than that observed in normal cells. This enhanced response in diabetic rat hepatocytes is neither potentiated by adding dibutyryl cAMP nor repressed by glucose. The absence of a glucose effect persists even when the endogenous cAMP content is lowered to normal levels. The results obtained in this study are consistent with those reported in other published studies of ferrochelatase activity. This adds more experimental evidence to support the concept that ferrochelatase is inducible. The results obtained suggest that ferrochelatase is more susceptible to induction with phenobarbital in diabetic rat hepatocytes than in normal rat hepatocytes. Fil:Cánepa, E.T. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pereda, M.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Llambías, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Grinstein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1992 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08298211_v70_n1_p26_Canepa http://hdl.handle.net/20.500.12110/paper_08298211_v70_n1_p26_Canepa |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
bucladesine ferrochelatase glucose messenger RNA phenobarbital animal article biosynthesis drug effect enzyme induction experimental diabetes mellitus gene expression regulation liver male metabolism rat rat strain Animals Bucladesine Diabetes Mellitus, Experimental Enzyme Induction Ferrochelatase Gene Expression Regulation Glucose Liver Male Phenobarbital Rats Rats, Inbred Strains RNA, Messenger |
| spellingShingle |
bucladesine ferrochelatase glucose messenger RNA phenobarbital animal article biosynthesis drug effect enzyme induction experimental diabetes mellitus gene expression regulation liver male metabolism rat rat strain Animals Bucladesine Diabetes Mellitus, Experimental Enzyme Induction Ferrochelatase Gene Expression Regulation Glucose Liver Male Phenobarbital Rats Rats, Inbred Strains RNA, Messenger Cánepa, Eduardo Tomás Páez Pereda, Marcelo Llambías, Elena Blanca Cecilia Grinstein, Moisés Regulation of phenobarbital-induced ferrochelatase mRNA activity by dibutyryl cAMP and glucose in normal and diabetic rat hepatocytes. |
| topic_facet |
bucladesine ferrochelatase glucose messenger RNA phenobarbital animal article biosynthesis drug effect enzyme induction experimental diabetes mellitus gene expression regulation liver male metabolism rat rat strain Animals Bucladesine Diabetes Mellitus, Experimental Enzyme Induction Ferrochelatase Gene Expression Regulation Glucose Liver Male Phenobarbital Rats Rats, Inbred Strains RNA, Messenger |
| description |
The induction of ferrochelatase activity by phenobarbital and its potentiation by dibutyryl cAMP assayed in normal rat hepatocytes are associated with increased activity of ferrochelatase mRNA. Glucose inhibits this stimulatory effect. This inhibition can be reversed with increasing concentrations of dibutyryl cAMP. The inducing effect exerted by phenobarbital on the activity of ferrochelatase mRNA in diabetic hepatocytes is greater than that observed in normal cells. This enhanced response in diabetic rat hepatocytes is neither potentiated by adding dibutyryl cAMP nor repressed by glucose. The absence of a glucose effect persists even when the endogenous cAMP content is lowered to normal levels. The results obtained in this study are consistent with those reported in other published studies of ferrochelatase activity. This adds more experimental evidence to support the concept that ferrochelatase is inducible. The results obtained suggest that ferrochelatase is more susceptible to induction with phenobarbital in diabetic rat hepatocytes than in normal rat hepatocytes. |
| author |
Cánepa, Eduardo Tomás Páez Pereda, Marcelo Llambías, Elena Blanca Cecilia Grinstein, Moisés |
| author_facet |
Cánepa, Eduardo Tomás Páez Pereda, Marcelo Llambías, Elena Blanca Cecilia Grinstein, Moisés |
| author_sort |
Cánepa, Eduardo Tomás |
| title |
Regulation of phenobarbital-induced ferrochelatase mRNA activity by dibutyryl cAMP and glucose in normal and diabetic rat hepatocytes. |
| title_short |
Regulation of phenobarbital-induced ferrochelatase mRNA activity by dibutyryl cAMP and glucose in normal and diabetic rat hepatocytes. |
| title_full |
Regulation of phenobarbital-induced ferrochelatase mRNA activity by dibutyryl cAMP and glucose in normal and diabetic rat hepatocytes. |
| title_fullStr |
Regulation of phenobarbital-induced ferrochelatase mRNA activity by dibutyryl cAMP and glucose in normal and diabetic rat hepatocytes. |
| title_full_unstemmed |
Regulation of phenobarbital-induced ferrochelatase mRNA activity by dibutyryl cAMP and glucose in normal and diabetic rat hepatocytes. |
| title_sort |
regulation of phenobarbital-induced ferrochelatase mrna activity by dibutyryl camp and glucose in normal and diabetic rat hepatocytes. |
| publishDate |
1992 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08298211_v70_n1_p26_Canepa http://hdl.handle.net/20.500.12110/paper_08298211_v70_n1_p26_Canepa |
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| _version_ |
1768542649781846016 |