Functional in vitro studies on the role and regulation of interleukin-6 in human somatotroph pituitary adenomas
Objective: Interleukin-6 (IL-6), a member of the gp130 cytokine family, is considered to be an important modulator of function and growth in endocrine anterior pituitary cells. In pituitary adenomas, where IL-6 is often produced by the tumour cells, it is thought to be involved in pituitary adenoma...
| Publicado: |
2003
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08044643_v149_n5_p455_Thiele http://hdl.handle.net/20.500.12110/paper_08044643_v149_n5_p455_Thiele |
| Aporte de: |
| id |
paper:paper_08044643_v149_n5_p455_Thiele |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_08044643_v149_n5_p455_Thiele2023-06-08T15:46:01Z Functional in vitro studies on the role and regulation of interleukin-6 in human somatotroph pituitary adenomas dexamethasone gonadorelin growth hormone interleukin 6 octreotide somatomedin C transforming growth factor beta1 adult article autocrine effect cell culture clinical article controlled study cytokine production cytokine release female growth hormone release hormone synthesis human human cell hypophysis adenoma immunohistology in vitro study male paracrine signaling pathophysiology priority journal protein function regulatory mechanism somatic cell transsphenoidal surgery Adenoma Adjuvants, Immunologic Adult Dose-Response Relationship, Drug Female Human Growth Hormone Humans Interleukin-6 Male Middle Aged Pituitary Neoplasms Tumor Cells, Cultured Objective: Interleukin-6 (IL-6), a member of the gp130 cytokine family, is considered to be an important modulator of function and growth in endocrine anterior pituitary cells. In pituitary adenomas, where IL-6 is often produced by the tumour cells, it is thought to be involved in pituitary adenoma pathophysiology via autocrine/paracrine mechanisms. Methods: We have studied in primary cell cultures of human somatotroph adenomas whether IL-6 stimulates growth hormone secretion and whether intratumoral IL-6 is affected by various IL-6-regulating factors. Results: Interleukin-6 stimulated GH secretion in 10 out of 11 somatotroph adenoma cultures (1.4- to 6.5-fold above basal levels). In comparative studies the GH-stimulatory potency of IL-6 was identical, or even stronger, than that of GHRH. In eight out of 11 adenoma cell cultures, IL-6 production was observed. This suggests that GH production might be stimulated by IL-6 in an autocrine/paracrine manner in these tumours. Dexamethasone strongly inhibited basal IL-6 secretion in all IL-6-producing adenoma cell cultures, whereas the IL-6 inhibitory or stimulatory action of other factors (octreotide, transforming growth factor-β1, insulin-like growth factor-I, pituitary adenylate cyclase-activating peptide and oestradiol) were heterogeneous in the different adenomas. Only transforming growth factor-α consistently stimulated IL-6 secretion in all of the adenomas studied. Conclusions: Intratumoral IL-6, which is differently regulated by various factors, might contribute to excessive GH production in the majority of somatotroph adenomas. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08044643_v149_n5_p455_Thiele http://hdl.handle.net/20.500.12110/paper_08044643_v149_n5_p455_Thiele |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
dexamethasone gonadorelin growth hormone interleukin 6 octreotide somatomedin C transforming growth factor beta1 adult article autocrine effect cell culture clinical article controlled study cytokine production cytokine release female growth hormone release hormone synthesis human human cell hypophysis adenoma immunohistology in vitro study male paracrine signaling pathophysiology priority journal protein function regulatory mechanism somatic cell transsphenoidal surgery Adenoma Adjuvants, Immunologic Adult Dose-Response Relationship, Drug Female Human Growth Hormone Humans Interleukin-6 Male Middle Aged Pituitary Neoplasms Tumor Cells, Cultured |
| spellingShingle |
dexamethasone gonadorelin growth hormone interleukin 6 octreotide somatomedin C transforming growth factor beta1 adult article autocrine effect cell culture clinical article controlled study cytokine production cytokine release female growth hormone release hormone synthesis human human cell hypophysis adenoma immunohistology in vitro study male paracrine signaling pathophysiology priority journal protein function regulatory mechanism somatic cell transsphenoidal surgery Adenoma Adjuvants, Immunologic Adult Dose-Response Relationship, Drug Female Human Growth Hormone Humans Interleukin-6 Male Middle Aged Pituitary Neoplasms Tumor Cells, Cultured Functional in vitro studies on the role and regulation of interleukin-6 in human somatotroph pituitary adenomas |
| topic_facet |
dexamethasone gonadorelin growth hormone interleukin 6 octreotide somatomedin C transforming growth factor beta1 adult article autocrine effect cell culture clinical article controlled study cytokine production cytokine release female growth hormone release hormone synthesis human human cell hypophysis adenoma immunohistology in vitro study male paracrine signaling pathophysiology priority journal protein function regulatory mechanism somatic cell transsphenoidal surgery Adenoma Adjuvants, Immunologic Adult Dose-Response Relationship, Drug Female Human Growth Hormone Humans Interleukin-6 Male Middle Aged Pituitary Neoplasms Tumor Cells, Cultured |
| description |
Objective: Interleukin-6 (IL-6), a member of the gp130 cytokine family, is considered to be an important modulator of function and growth in endocrine anterior pituitary cells. In pituitary adenomas, where IL-6 is often produced by the tumour cells, it is thought to be involved in pituitary adenoma pathophysiology via autocrine/paracrine mechanisms. Methods: We have studied in primary cell cultures of human somatotroph adenomas whether IL-6 stimulates growth hormone secretion and whether intratumoral IL-6 is affected by various IL-6-regulating factors. Results: Interleukin-6 stimulated GH secretion in 10 out of 11 somatotroph adenoma cultures (1.4- to 6.5-fold above basal levels). In comparative studies the GH-stimulatory potency of IL-6 was identical, or even stronger, than that of GHRH. In eight out of 11 adenoma cell cultures, IL-6 production was observed. This suggests that GH production might be stimulated by IL-6 in an autocrine/paracrine manner in these tumours. Dexamethasone strongly inhibited basal IL-6 secretion in all IL-6-producing adenoma cell cultures, whereas the IL-6 inhibitory or stimulatory action of other factors (octreotide, transforming growth factor-β1, insulin-like growth factor-I, pituitary adenylate cyclase-activating peptide and oestradiol) were heterogeneous in the different adenomas. Only transforming growth factor-α consistently stimulated IL-6 secretion in all of the adenomas studied. Conclusions: Intratumoral IL-6, which is differently regulated by various factors, might contribute to excessive GH production in the majority of somatotroph adenomas. |
| title |
Functional in vitro studies on the role and regulation of interleukin-6 in human somatotroph pituitary adenomas |
| title_short |
Functional in vitro studies on the role and regulation of interleukin-6 in human somatotroph pituitary adenomas |
| title_full |
Functional in vitro studies on the role and regulation of interleukin-6 in human somatotroph pituitary adenomas |
| title_fullStr |
Functional in vitro studies on the role and regulation of interleukin-6 in human somatotroph pituitary adenomas |
| title_full_unstemmed |
Functional in vitro studies on the role and regulation of interleukin-6 in human somatotroph pituitary adenomas |
| title_sort |
functional in vitro studies on the role and regulation of interleukin-6 in human somatotroph pituitary adenomas |
| publishDate |
2003 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08044643_v149_n5_p455_Thiele http://hdl.handle.net/20.500.12110/paper_08044643_v149_n5_p455_Thiele |
| _version_ |
1768544691125485568 |