One-Photon Lithography for High-Quality Lipid Bilayer Micropatterns
A relevant question in cell biology with broad implications in biomedicine is how the organization and dynamics of interacting membranes modulate signaling cascades that involve cell-cell contact. The functionalization of surfaces with supported lipid bilayers containing tethered proteins is a parti...
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2015
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07437463_v31_n43_p11943_Sanchez http://hdl.handle.net/20.500.12110/paper_07437463_v31_n43_p11943_Sanchez |
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paper:paper_07437463_v31_n43_p11943_Sanchez |
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paper:paper_07437463_v31_n43_p11943_Sanchez2023-06-08T15:45:02Z One-Photon Lithography for High-Quality Lipid Bilayer Micropatterns Cell signaling Cells Cytology Electron device manufacture Fluorescence Fluorescence spectroscopy Photobleaching Photons Proteins Spectroscopic analysis Direct interactions Fluorescence Correlation Spectroscopy Fluorescence recovery after photobleaching Functionalizations Laser scanning microscope Microcontact printing techniques Signaling cascades Supported lipid bilayers Lipid bilayers ligand lipid bilayer chemistry lipid bilayer photon spectrofluorometry Ligands Lipid Bilayers Photons Spectrometry, Fluorescence A relevant question in cell biology with broad implications in biomedicine is how the organization and dynamics of interacting membranes modulate signaling cascades that involve cell-cell contact. The functionalization of surfaces with supported lipid bilayers containing tethered proteins is a particularly useful method to present ligands with membrane-like mobility to cells. Here, we present a method to generate micrometer-sized patches of lipid bilayers decorated with proteins. The method uses an economic microcontact printing technique based on one-photon lithography that can be easily implemented in a commercial laser scanning microscope. We verified that both proteins and lipids freely diffuse within the patterned bilayer, as assessed by z-scan fluorescence correlation spectroscopy and fluorescence recovery after photobleaching. These results suggest that the supported lipid bilayer patterns constitute an optimal system to explore processes involving direct interactions between cells. We also illustrate possible applications of this method by exploring the interaction of cells expressing the Fas receptor and patterns of lipid bilayers containing an agonist antibody against Fas. © 2015 American Chemical Society. 2015 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07437463_v31_n43_p11943_Sanchez http://hdl.handle.net/20.500.12110/paper_07437463_v31_n43_p11943_Sanchez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cell signaling Cells Cytology Electron device manufacture Fluorescence Fluorescence spectroscopy Photobleaching Photons Proteins Spectroscopic analysis Direct interactions Fluorescence Correlation Spectroscopy Fluorescence recovery after photobleaching Functionalizations Laser scanning microscope Microcontact printing techniques Signaling cascades Supported lipid bilayers Lipid bilayers ligand lipid bilayer chemistry lipid bilayer photon spectrofluorometry Ligands Lipid Bilayers Photons Spectrometry, Fluorescence |
| spellingShingle |
Cell signaling Cells Cytology Electron device manufacture Fluorescence Fluorescence spectroscopy Photobleaching Photons Proteins Spectroscopic analysis Direct interactions Fluorescence Correlation Spectroscopy Fluorescence recovery after photobleaching Functionalizations Laser scanning microscope Microcontact printing techniques Signaling cascades Supported lipid bilayers Lipid bilayers ligand lipid bilayer chemistry lipid bilayer photon spectrofluorometry Ligands Lipid Bilayers Photons Spectrometry, Fluorescence One-Photon Lithography for High-Quality Lipid Bilayer Micropatterns |
| topic_facet |
Cell signaling Cells Cytology Electron device manufacture Fluorescence Fluorescence spectroscopy Photobleaching Photons Proteins Spectroscopic analysis Direct interactions Fluorescence Correlation Spectroscopy Fluorescence recovery after photobleaching Functionalizations Laser scanning microscope Microcontact printing techniques Signaling cascades Supported lipid bilayers Lipid bilayers ligand lipid bilayer chemistry lipid bilayer photon spectrofluorometry Ligands Lipid Bilayers Photons Spectrometry, Fluorescence |
| description |
A relevant question in cell biology with broad implications in biomedicine is how the organization and dynamics of interacting membranes modulate signaling cascades that involve cell-cell contact. The functionalization of surfaces with supported lipid bilayers containing tethered proteins is a particularly useful method to present ligands with membrane-like mobility to cells. Here, we present a method to generate micrometer-sized patches of lipid bilayers decorated with proteins. The method uses an economic microcontact printing technique based on one-photon lithography that can be easily implemented in a commercial laser scanning microscope. We verified that both proteins and lipids freely diffuse within the patterned bilayer, as assessed by z-scan fluorescence correlation spectroscopy and fluorescence recovery after photobleaching. These results suggest that the supported lipid bilayer patterns constitute an optimal system to explore processes involving direct interactions between cells. We also illustrate possible applications of this method by exploring the interaction of cells expressing the Fas receptor and patterns of lipid bilayers containing an agonist antibody against Fas. © 2015 American Chemical Society. |
| title |
One-Photon Lithography for High-Quality Lipid Bilayer Micropatterns |
| title_short |
One-Photon Lithography for High-Quality Lipid Bilayer Micropatterns |
| title_full |
One-Photon Lithography for High-Quality Lipid Bilayer Micropatterns |
| title_fullStr |
One-Photon Lithography for High-Quality Lipid Bilayer Micropatterns |
| title_full_unstemmed |
One-Photon Lithography for High-Quality Lipid Bilayer Micropatterns |
| title_sort |
one-photon lithography for high-quality lipid bilayer micropatterns |
| publishDate |
2015 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07437463_v31_n43_p11943_Sanchez http://hdl.handle.net/20.500.12110/paper_07437463_v31_n43_p11943_Sanchez |
| _version_ |
1768543186127421440 |