Photophysics on surfaces: Determination of absolute fluorescence quantum yields from reflectance spectra
A method for the calculation of absolute fluorescence quantum yields for dyes attached to solid particles based on reflectance measurements is reported. The same procedure allows calculation of true reflectance spectra (free of fluorescence) for highly fluorescent materials as well. Samples of cresy...
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paper:paper_07437463_v20_n9_p3690_Mirenda2023-06-08T15:44:53Z Photophysics on surfaces: Determination of absolute fluorescence quantum yields from reflectance spectra Mirenda, Martín Lagorio, María Gabriela Cellulose Diffusion Fluorescence Light absorption Light emission Light reflection Light scattering Photochemical reactions Spectroscopic analysis Dye aggregation Excitation radiation Fluorescence quantum yields Stokes shift Dyes Cellulose Diffusion Dyes Light Absorption Optical Scattering Photochemistry Spectrochemical Analysis A method for the calculation of absolute fluorescence quantum yields for dyes attached to solid particles based on reflectance measurements is reported. The same procedure allows calculation of true reflectance spectra (free of fluorescence) for highly fluorescent materials as well. Samples of cresyl violet were immobilized by adsorption on microgranular cellulose in the concentration range 4.5 × 10-9 to 3.8 × 10-6 mol g -1. Diffuse and total reflectance spectra were recorded with and without insertion of an optical absorption filter between the output of the integrating sphere of a reflectance spectrometer and the photodetector in order to block fluorescence partially. From these data, the relative emission spectrum of the dye, the filter transmission spectrum, and the detector sensitivity, true reflectances and absolute fluorescence quantum yields were recovered. Observed fluorescence quantum yields, affected by dye aggregation and inner filter effects, were concentration and wavelength dependent, ranging approximately between 0.1 and 0.6. The analysis of remission function spectra showed that dye aggregation is negligible up to a concentration of 1.41 × 10-7 mol g-1. Fluorescence data were corrected for reemission and reabsorption using a suitable model [Lagorio, M. G.; Dicelio, L. E.; Litter, M. I.; San Román, E. J. Chem. Soc., Faraday Trans. 1998, 94, 419]. Application of this model to samples showing no aggregation yielded a wavelength-independent true fluorescence quantum yield of 0.60 ± 0.05, similar to values found in solution. The usage of cresyl violet as a reference for the evaluation of fluorescence quantum yields for weakly fluorescing samples in the solid phase is discussed. Fil:Mirenda, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lagorio, M.G. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2004 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07437463_v20_n9_p3690_Mirenda http://hdl.handle.net/20.500.12110/paper_07437463_v20_n9_p3690_Mirenda |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Cellulose Diffusion Fluorescence Light absorption Light emission Light reflection Light scattering Photochemical reactions Spectroscopic analysis Dye aggregation Excitation radiation Fluorescence quantum yields Stokes shift Dyes Cellulose Diffusion Dyes Light Absorption Optical Scattering Photochemistry Spectrochemical Analysis |
spellingShingle |
Cellulose Diffusion Fluorescence Light absorption Light emission Light reflection Light scattering Photochemical reactions Spectroscopic analysis Dye aggregation Excitation radiation Fluorescence quantum yields Stokes shift Dyes Cellulose Diffusion Dyes Light Absorption Optical Scattering Photochemistry Spectrochemical Analysis Mirenda, Martín Lagorio, María Gabriela Photophysics on surfaces: Determination of absolute fluorescence quantum yields from reflectance spectra |
topic_facet |
Cellulose Diffusion Fluorescence Light absorption Light emission Light reflection Light scattering Photochemical reactions Spectroscopic analysis Dye aggregation Excitation radiation Fluorescence quantum yields Stokes shift Dyes Cellulose Diffusion Dyes Light Absorption Optical Scattering Photochemistry Spectrochemical Analysis |
description |
A method for the calculation of absolute fluorescence quantum yields for dyes attached to solid particles based on reflectance measurements is reported. The same procedure allows calculation of true reflectance spectra (free of fluorescence) for highly fluorescent materials as well. Samples of cresyl violet were immobilized by adsorption on microgranular cellulose in the concentration range 4.5 × 10-9 to 3.8 × 10-6 mol g -1. Diffuse and total reflectance spectra were recorded with and without insertion of an optical absorption filter between the output of the integrating sphere of a reflectance spectrometer and the photodetector in order to block fluorescence partially. From these data, the relative emission spectrum of the dye, the filter transmission spectrum, and the detector sensitivity, true reflectances and absolute fluorescence quantum yields were recovered. Observed fluorescence quantum yields, affected by dye aggregation and inner filter effects, were concentration and wavelength dependent, ranging approximately between 0.1 and 0.6. The analysis of remission function spectra showed that dye aggregation is negligible up to a concentration of 1.41 × 10-7 mol g-1. Fluorescence data were corrected for reemission and reabsorption using a suitable model [Lagorio, M. G.; Dicelio, L. E.; Litter, M. I.; San Román, E. J. Chem. Soc., Faraday Trans. 1998, 94, 419]. Application of this model to samples showing no aggregation yielded a wavelength-independent true fluorescence quantum yield of 0.60 ± 0.05, similar to values found in solution. The usage of cresyl violet as a reference for the evaluation of fluorescence quantum yields for weakly fluorescing samples in the solid phase is discussed. |
author |
Mirenda, Martín Lagorio, María Gabriela |
author_facet |
Mirenda, Martín Lagorio, María Gabriela |
author_sort |
Mirenda, Martín |
title |
Photophysics on surfaces: Determination of absolute fluorescence quantum yields from reflectance spectra |
title_short |
Photophysics on surfaces: Determination of absolute fluorescence quantum yields from reflectance spectra |
title_full |
Photophysics on surfaces: Determination of absolute fluorescence quantum yields from reflectance spectra |
title_fullStr |
Photophysics on surfaces: Determination of absolute fluorescence quantum yields from reflectance spectra |
title_full_unstemmed |
Photophysics on surfaces: Determination of absolute fluorescence quantum yields from reflectance spectra |
title_sort |
photophysics on surfaces: determination of absolute fluorescence quantum yields from reflectance spectra |
publishDate |
2004 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07437463_v20_n9_p3690_Mirenda http://hdl.handle.net/20.500.12110/paper_07437463_v20_n9_p3690_Mirenda |
work_keys_str_mv |
AT mirendamartin photophysicsonsurfacesdeterminationofabsolutefluorescencequantumyieldsfromreflectancespectra AT lagoriomariagabriela photophysicsonsurfacesdeterminationofabsolutefluorescencequantumyieldsfromreflectancespectra |
_version_ |
1768546680494358528 |