Electrochemical behavior of nitrate reductase immobilized in self-assembled structures with redox polyviologen
We report on a novel bioelectrode based on self-assembled multilayers of nitrate reductase (NR) intercalated with a cationic viologen-functionalized polyvinylpyridinium (PV) polymer specially synthesized for this purpose. Different samples of polymer proved to have varying substitution ratios, accor...
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2003
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07437463_v19_n9_p3864_Ferreyra http://hdl.handle.net/20.500.12110/paper_07437463_v19_n9_p3864_Ferreyra |
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paper:paper_07437463_v19_n9_p3864_Ferreyra2023-06-08T15:44:52Z Electrochemical behavior of nitrate reductase immobilized in self-assembled structures with redox polyviologen Calvo, Ernesto Julio Cyclic voltammetry Electrostatics Ion exchange Ionic strength Nuclear magnetic resonance Self assembly Redox polyviologen Nitrates We report on a novel bioelectrode based on self-assembled multilayers of nitrate reductase (NR) intercalated with a cationic viologen-functionalized polyvinylpyridinium (PV) polymer specially synthesized for this purpose. Different samples of polymer proved to have varying substitution ratios, according to NMR experiments. The electrostatic self-assemblies were built up on a thiol-modified gold surface using a strategy previously described by Hodak et al. The electrochemical behavior of PV and the response of the electrodes toward nitrate were followed by cyclic voltammetry. The catalytic currents obtained were proportional to the number of NR-immobilized layers, as well as to the polymer substitution degree, proving that the enzyme remained active under immobilization conditions and that it can be regenerated by PV redox moieties. Experiments with quartz crystal microbalance electroacoustic impedance were carried out to follow quantitatively the multilayer film formation. The kinetics of the adsorption processes and the influence of the ionic strength are investigated. The evaluation of water and ion exchange during the reduction of PV is also achieved from electroacoustic and electrochemical experiments. Results indicate an important water exchange associated with the polymer redox behavior. Fil:Calvo, E.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2003 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07437463_v19_n9_p3864_Ferreyra http://hdl.handle.net/20.500.12110/paper_07437463_v19_n9_p3864_Ferreyra |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cyclic voltammetry Electrostatics Ion exchange Ionic strength Nuclear magnetic resonance Self assembly Redox polyviologen Nitrates |
| spellingShingle |
Cyclic voltammetry Electrostatics Ion exchange Ionic strength Nuclear magnetic resonance Self assembly Redox polyviologen Nitrates Calvo, Ernesto Julio Electrochemical behavior of nitrate reductase immobilized in self-assembled structures with redox polyviologen |
| topic_facet |
Cyclic voltammetry Electrostatics Ion exchange Ionic strength Nuclear magnetic resonance Self assembly Redox polyviologen Nitrates |
| description |
We report on a novel bioelectrode based on self-assembled multilayers of nitrate reductase (NR) intercalated with a cationic viologen-functionalized polyvinylpyridinium (PV) polymer specially synthesized for this purpose. Different samples of polymer proved to have varying substitution ratios, according to NMR experiments. The electrostatic self-assemblies were built up on a thiol-modified gold surface using a strategy previously described by Hodak et al. The electrochemical behavior of PV and the response of the electrodes toward nitrate were followed by cyclic voltammetry. The catalytic currents obtained were proportional to the number of NR-immobilized layers, as well as to the polymer substitution degree, proving that the enzyme remained active under immobilization conditions and that it can be regenerated by PV redox moieties. Experiments with quartz crystal microbalance electroacoustic impedance were carried out to follow quantitatively the multilayer film formation. The kinetics of the adsorption processes and the influence of the ionic strength are investigated. The evaluation of water and ion exchange during the reduction of PV is also achieved from electroacoustic and electrochemical experiments. Results indicate an important water exchange associated with the polymer redox behavior. |
| author |
Calvo, Ernesto Julio |
| author_facet |
Calvo, Ernesto Julio |
| author_sort |
Calvo, Ernesto Julio |
| title |
Electrochemical behavior of nitrate reductase immobilized in self-assembled structures with redox polyviologen |
| title_short |
Electrochemical behavior of nitrate reductase immobilized in self-assembled structures with redox polyviologen |
| title_full |
Electrochemical behavior of nitrate reductase immobilized in self-assembled structures with redox polyviologen |
| title_fullStr |
Electrochemical behavior of nitrate reductase immobilized in self-assembled structures with redox polyviologen |
| title_full_unstemmed |
Electrochemical behavior of nitrate reductase immobilized in self-assembled structures with redox polyviologen |
| title_sort |
electrochemical behavior of nitrate reductase immobilized in self-assembled structures with redox polyviologen |
| publishDate |
2003 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07437463_v19_n9_p3864_Ferreyra http://hdl.handle.net/20.500.12110/paper_07437463_v19_n9_p3864_Ferreyra |
| work_keys_str_mv |
AT calvoernestojulio electrochemicalbehaviorofnitratereductaseimmobilizedinselfassembledstructureswithredoxpolyviologen |
| _version_ |
1768544916769603584 |