Dose-dependence of the effects of corticosterone and the non- glucocorticoid, 18-hydroxycorticosterone, on the brush-border Na+/K+ exchanger

Up to now, only glucocorticoids were thought to act on the renal proximal Na+/H+ exchanger. Using fluorimetric techniques we studied the kinetics of Na+/H+ exchange in brush border vesicles from ADX rats treated with increasing doses of corticosterone (B) and 18-hydroxycorticosterone (18OHB). Signif...

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Detalles Bibliográficos
Autor principal: Calvo, Juan Carlos
Publicado: 1998
Materias:
18 hydroxycorticosterone
adenosine triphosphatase (potassium sodium)
corticosterone
animal tissue
brush border
conference paper
dose response
fluorometry
glycogen synthesis
hormone action
male
nonhuman
potassium transport
priority journal
proton transport
rat
sodium transport
transport kinetics
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07435800_v24_n3-4_p601_Igarreta
http://hdl.handle.net/20.500.12110/paper_07435800_v24_n3-4_p601_Igarreta
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_07435800_v24_n3-4_p601_Igarreta
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spelling paper:paper_07435800_v24_n3-4_p601_Igarreta2023-06-08T15:44:46Z Dose-dependence of the effects of corticosterone and the non- glucocorticoid, 18-hydroxycorticosterone, on the brush-border Na+/K+ exchanger Calvo, Juan Carlos 18 hydroxycorticosterone adenosine triphosphatase (potassium sodium) corticosterone animal tissue brush border conference paper dose response fluorometry glycogen synthesis hormone action male nonhuman potassium transport priority journal proton transport rat sodium transport transport kinetics Up to now, only glucocorticoids were thought to act on the renal proximal Na+/H+ exchanger. Using fluorimetric techniques we studied the kinetics of Na+/H+ exchange in brush border vesicles from ADX rats treated with increasing doses of corticosterone (B) and 18-hydroxycorticosterone (18OHB). Significant linear correlations were obtained when the Vmax of each treatment were plotted against log doses. 18OHB exhibits a slightly higher sensitivity than B and log-dose responses were steeper for 18OHB than for B treated rats. Differences between both treatments were highly significant at the 4.8 μg/100 g level, corresponding to the physiological blood level of 18OHB. Physiological doses of both steroids elicited equal Na+/H+ exchange- responses. 18OHB is not a glucocorticoid since even 88μg/100g did not promote hepatic glycogen deposition while the same dose of B increases glycogen deposits 3.5-fold. These results demonstrate the importance of the Na+/H+ exchanger as a mediator between corticoid action and H+ transport and that of the non-glucocorticoid 18OHB in this process. Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1998 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07435800_v24_n3-4_p601_Igarreta http://hdl.handle.net/20.500.12110/paper_07435800_v24_n3-4_p601_Igarreta
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 18 hydroxycorticosterone
adenosine triphosphatase (potassium sodium)
corticosterone
animal tissue
brush border
conference paper
dose response
fluorometry
glycogen synthesis
hormone action
male
nonhuman
potassium transport
priority journal
proton transport
rat
sodium transport
transport kinetics
spellingShingle 18 hydroxycorticosterone
adenosine triphosphatase (potassium sodium)
corticosterone
animal tissue
brush border
conference paper
dose response
fluorometry
glycogen synthesis
hormone action
male
nonhuman
potassium transport
priority journal
proton transport
rat
sodium transport
transport kinetics
Calvo, Juan Carlos
Dose-dependence of the effects of corticosterone and the non- glucocorticoid, 18-hydroxycorticosterone, on the brush-border Na+/K+ exchanger
topic_facet 18 hydroxycorticosterone
adenosine triphosphatase (potassium sodium)
corticosterone
animal tissue
brush border
conference paper
dose response
fluorometry
glycogen synthesis
hormone action
male
nonhuman
potassium transport
priority journal
proton transport
rat
sodium transport
transport kinetics
description Up to now, only glucocorticoids were thought to act on the renal proximal Na+/H+ exchanger. Using fluorimetric techniques we studied the kinetics of Na+/H+ exchange in brush border vesicles from ADX rats treated with increasing doses of corticosterone (B) and 18-hydroxycorticosterone (18OHB). Significant linear correlations were obtained when the Vmax of each treatment were plotted against log doses. 18OHB exhibits a slightly higher sensitivity than B and log-dose responses were steeper for 18OHB than for B treated rats. Differences between both treatments were highly significant at the 4.8 μg/100 g level, corresponding to the physiological blood level of 18OHB. Physiological doses of both steroids elicited equal Na+/H+ exchange- responses. 18OHB is not a glucocorticoid since even 88μg/100g did not promote hepatic glycogen deposition while the same dose of B increases glycogen deposits 3.5-fold. These results demonstrate the importance of the Na+/H+ exchanger as a mediator between corticoid action and H+ transport and that of the non-glucocorticoid 18OHB in this process.
author Calvo, Juan Carlos
author_facet Calvo, Juan Carlos
author_sort Calvo, Juan Carlos
title Dose-dependence of the effects of corticosterone and the non- glucocorticoid, 18-hydroxycorticosterone, on the brush-border Na+/K+ exchanger
title_short Dose-dependence of the effects of corticosterone and the non- glucocorticoid, 18-hydroxycorticosterone, on the brush-border Na+/K+ exchanger
title_full Dose-dependence of the effects of corticosterone and the non- glucocorticoid, 18-hydroxycorticosterone, on the brush-border Na+/K+ exchanger
title_fullStr Dose-dependence of the effects of corticosterone and the non- glucocorticoid, 18-hydroxycorticosterone, on the brush-border Na+/K+ exchanger
title_full_unstemmed Dose-dependence of the effects of corticosterone and the non- glucocorticoid, 18-hydroxycorticosterone, on the brush-border Na+/K+ exchanger
title_sort dose-dependence of the effects of corticosterone and the non- glucocorticoid, 18-hydroxycorticosterone, on the brush-border na+/k+ exchanger
publishDate 1998
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07435800_v24_n3-4_p601_Igarreta
http://hdl.handle.net/20.500.12110/paper_07435800_v24_n3-4_p601_Igarreta
work_keys_str_mv AT calvojuancarlos dosedependenceoftheeffectsofcorticosteroneandthenonglucocorticoid18hydroxycorticosteroneonthebrushbordernakexchanger
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