Corticosteroid synthesis in the central nervous system
The possibility that adrenocorticosteroids might be synthesized in the central nervous system was assessed by RT-PCR using primers for the CYP11B1 gene which codes for 11β-hydroxylase, the enzyme responsible for corticosterone and cortisol formation in the zona fasciculata, incubation of minces of s...
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1996
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07435800_v22_n4_p463_GomezSanchez http://hdl.handle.net/20.500.12110/paper_07435800_v22_n4_p463_GomezSanchez |
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paper:paper_07435800_v22_n4_p463_GomezSanchez2023-06-08T15:44:45Z Corticosteroid synthesis in the central nervous system Cozza, Eduardo Néstor 19 ethynyldeoxycorticosterone corticosterone dehydrocorticosterone steroid 11beta monooxygenase steroid 11beta monooxygenase inhibitor unclassified drug animal cell animal tissue blood pressure measurement central nervous system function conference paper dose response enzyme mechanism hypertension inhibition kinetics intracerebroventricular drug administration male nonhuman priority journal rat steroidogenesis tissue distribution The possibility that adrenocorticosteroids might be synthesized in the central nervous system was assessed by RT-PCR using primers for the CYP11B1 gene which codes for 11β-hydroxylase, the enzyme responsible for corticosterone and cortisol formation in the zona fasciculata, incubation of minces of several areas of the brain with 3H-DOC and measuring steroid metabolites, and determining the effect of the intracerebroventricular infusion of the 11β-hydroxylase mechanism-based inhibitor 19- ethynyldeoxycorticosterone upon the salt-induced increase in blood pressure in SS/jr rats. Significant, though small relative to the adrenal, amounts of mRNA for 11β-hydroxylase was found in the aorta, cerebrum, cerebellum, hippocampus, hypothalamus and amygdala, but not in the heart. Brain minces converted 3H-DOC to corticosterone and 11-dehydrocorticosterone to a greater degree than to 18-OH-DOC. The effect of 19- ethynyldeoxycorticosterone was dose dependent, with the lower doses preventing salt-induced hypertension and the higher doses having no effect or increasing the blood pressure. Fil:Cozza, E.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1996 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07435800_v22_n4_p463_GomezSanchez http://hdl.handle.net/20.500.12110/paper_07435800_v22_n4_p463_GomezSanchez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
19 ethynyldeoxycorticosterone corticosterone dehydrocorticosterone steroid 11beta monooxygenase steroid 11beta monooxygenase inhibitor unclassified drug animal cell animal tissue blood pressure measurement central nervous system function conference paper dose response enzyme mechanism hypertension inhibition kinetics intracerebroventricular drug administration male nonhuman priority journal rat steroidogenesis tissue distribution |
| spellingShingle |
19 ethynyldeoxycorticosterone corticosterone dehydrocorticosterone steroid 11beta monooxygenase steroid 11beta monooxygenase inhibitor unclassified drug animal cell animal tissue blood pressure measurement central nervous system function conference paper dose response enzyme mechanism hypertension inhibition kinetics intracerebroventricular drug administration male nonhuman priority journal rat steroidogenesis tissue distribution Cozza, Eduardo Néstor Corticosteroid synthesis in the central nervous system |
| topic_facet |
19 ethynyldeoxycorticosterone corticosterone dehydrocorticosterone steroid 11beta monooxygenase steroid 11beta monooxygenase inhibitor unclassified drug animal cell animal tissue blood pressure measurement central nervous system function conference paper dose response enzyme mechanism hypertension inhibition kinetics intracerebroventricular drug administration male nonhuman priority journal rat steroidogenesis tissue distribution |
| description |
The possibility that adrenocorticosteroids might be synthesized in the central nervous system was assessed by RT-PCR using primers for the CYP11B1 gene which codes for 11β-hydroxylase, the enzyme responsible for corticosterone and cortisol formation in the zona fasciculata, incubation of minces of several areas of the brain with 3H-DOC and measuring steroid metabolites, and determining the effect of the intracerebroventricular infusion of the 11β-hydroxylase mechanism-based inhibitor 19- ethynyldeoxycorticosterone upon the salt-induced increase in blood pressure in SS/jr rats. Significant, though small relative to the adrenal, amounts of mRNA for 11β-hydroxylase was found in the aorta, cerebrum, cerebellum, hippocampus, hypothalamus and amygdala, but not in the heart. Brain minces converted 3H-DOC to corticosterone and 11-dehydrocorticosterone to a greater degree than to 18-OH-DOC. The effect of 19- ethynyldeoxycorticosterone was dose dependent, with the lower doses preventing salt-induced hypertension and the higher doses having no effect or increasing the blood pressure. |
| author |
Cozza, Eduardo Néstor |
| author_facet |
Cozza, Eduardo Néstor |
| author_sort |
Cozza, Eduardo Néstor |
| title |
Corticosteroid synthesis in the central nervous system |
| title_short |
Corticosteroid synthesis in the central nervous system |
| title_full |
Corticosteroid synthesis in the central nervous system |
| title_fullStr |
Corticosteroid synthesis in the central nervous system |
| title_full_unstemmed |
Corticosteroid synthesis in the central nervous system |
| title_sort |
corticosteroid synthesis in the central nervous system |
| publishDate |
1996 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07435800_v22_n4_p463_GomezSanchez http://hdl.handle.net/20.500.12110/paper_07435800_v22_n4_p463_GomezSanchez |
| work_keys_str_mv |
AT cozzaeduardonestor corticosteroidsynthesisinthecentralnervoussystem |
| _version_ |
1768545883720253440 |