High δ-aminolevulinic acid uptake in rat cerebral cortex: effect on porphyrin biosynthesis

The response of nerve cells to high exogenous aminolevulinic acid (ALA) concentrations was studied by examining the changes in its uptake and in porphyrin biosynthesis. ALA was shown to be taken up by cerebral cortex particles by a non-saturable process. As opposed to other previously described expe...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Juknat, Adela Ana, Batlle, Alcira María del Carmen
Publicado:	1995
Materias:	Acute porphyric attac AIP brain tissue model ALA accumulation ALA uptake Cerebral cortex particles Glucose uptake PBG accumulation Porphyrin biosynthesis aminolevulinic acid glucose porphyrin animal tissue article bioaccumulation brain cortex controlled study male nerve cell nonhuman porphyria priority journal protein synthesis rat Aminolevulinic Acid Animal Cerebral Cortex Chromatography, High Pressure Liquid Glucose Heme In Vitro Male Porphyrins Rats Spectrometry, Fluorescence Support, Non-U.S. Gov't
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07428413_v111_n1_p143_Juknat http://hdl.handle.net/20.500.12110/paper_07428413_v111_n1_p143_Juknat
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_07428413_v111_n1_p143_Juknat
record_format	dspace
spelling	paper:paper_07428413_v111_n1_p143_Juknat2023-06-08T15:44:45Z High δ-aminolevulinic acid uptake in rat cerebral cortex: effect on porphyrin biosynthesis Juknat, Adela Ana Batlle, Alcira María del Carmen Acute porphyric attac AIP brain tissue model ALA accumulation ALA uptake Cerebral cortex particles Glucose uptake PBG accumulation Porphyrin biosynthesis aminolevulinic acid glucose porphyrin animal tissue article bioaccumulation brain cortex controlled study male nerve cell nonhuman porphyria priority journal protein synthesis rat Aminolevulinic Acid Animal Cerebral Cortex Chromatography, High Pressure Liquid Glucose Heme In Vitro Male Porphyrins Rats Spectrometry, Fluorescence Support, Non-U.S. Gov't The response of nerve cells to high exogenous aminolevulinic acid (ALA) concentrations was studied by examining the changes in its uptake and in porphyrin biosynthesis. ALA was shown to be taken up by cerebral cortex particles by a non-saturable process. As opposed to other previously described experimental systems, it was also observed that 84-87% of porphyrins formed was found within the cells. Exposure of cerebral cortex particles to high exogenous ALA (0.8-4.0 mM) showed that ALA can be accumulated in relatively high concentrations in brain cells (21.04 ± 1.05 nmol/mg protein). Under these experimental conditions, porphyrin biosynthesis was found to be markedly inhibited (52%). 2.4 mM ALA caused an initial stimulation of glucose uptake after 1 hr incubation and a later fall to below control values, being consistent with the fact that acute porphyric crisis could be precipitated by the action of ALA on energy metabolism. ALA toxicity could be due both to its accumulation in the cells and to deficient heme concentrations, with an additional effect on glucose metabolism. These findings provide the basis for a useful brain tissue model to investigate the nature of the metabolic mechanisms occurring in acute intermittent porphyria (AIP) patients. © 1995. Fil:Juknat, A.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:del Carmen Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1995 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07428413_v111_n1_p143_Juknat http://hdl.handle.net/20.500.12110/paper_07428413_v111_n1_p143_Juknat
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Acute porphyric attac AIP brain tissue model ALA accumulation ALA uptake Cerebral cortex particles Glucose uptake PBG accumulation Porphyrin biosynthesis aminolevulinic acid glucose porphyrin animal tissue article bioaccumulation brain cortex controlled study male nerve cell nonhuman porphyria priority journal protein synthesis rat Aminolevulinic Acid Animal Cerebral Cortex Chromatography, High Pressure Liquid Glucose Heme In Vitro Male Porphyrins Rats Spectrometry, Fluorescence Support, Non-U.S. Gov't
spellingShingle	Acute porphyric attac AIP brain tissue model ALA accumulation ALA uptake Cerebral cortex particles Glucose uptake PBG accumulation Porphyrin biosynthesis aminolevulinic acid glucose porphyrin animal tissue article bioaccumulation brain cortex controlled study male nerve cell nonhuman porphyria priority journal protein synthesis rat Aminolevulinic Acid Animal Cerebral Cortex Chromatography, High Pressure Liquid Glucose Heme In Vitro Male Porphyrins Rats Spectrometry, Fluorescence Support, Non-U.S. Gov't Juknat, Adela Ana Batlle, Alcira María del Carmen High δ-aminolevulinic acid uptake in rat cerebral cortex: effect on porphyrin biosynthesis
topic_facet	Acute porphyric attac AIP brain tissue model ALA accumulation ALA uptake Cerebral cortex particles Glucose uptake PBG accumulation Porphyrin biosynthesis aminolevulinic acid glucose porphyrin animal tissue article bioaccumulation brain cortex controlled study male nerve cell nonhuman porphyria priority journal protein synthesis rat Aminolevulinic Acid Animal Cerebral Cortex Chromatography, High Pressure Liquid Glucose Heme In Vitro Male Porphyrins Rats Spectrometry, Fluorescence Support, Non-U.S. Gov't
description	The response of nerve cells to high exogenous aminolevulinic acid (ALA) concentrations was studied by examining the changes in its uptake and in porphyrin biosynthesis. ALA was shown to be taken up by cerebral cortex particles by a non-saturable process. As opposed to other previously described experimental systems, it was also observed that 84-87% of porphyrins formed was found within the cells. Exposure of cerebral cortex particles to high exogenous ALA (0.8-4.0 mM) showed that ALA can be accumulated in relatively high concentrations in brain cells (21.04 ± 1.05 nmol/mg protein). Under these experimental conditions, porphyrin biosynthesis was found to be markedly inhibited (52%). 2.4 mM ALA caused an initial stimulation of glucose uptake after 1 hr incubation and a later fall to below control values, being consistent with the fact that acute porphyric crisis could be precipitated by the action of ALA on energy metabolism. ALA toxicity could be due both to its accumulation in the cells and to deficient heme concentrations, with an additional effect on glucose metabolism. These findings provide the basis for a useful brain tissue model to investigate the nature of the metabolic mechanisms occurring in acute intermittent porphyria (AIP) patients. © 1995.
author	Juknat, Adela Ana Batlle, Alcira María del Carmen
author_facet	Juknat, Adela Ana Batlle, Alcira María del Carmen
author_sort	Juknat, Adela Ana
title	High δ-aminolevulinic acid uptake in rat cerebral cortex: effect on porphyrin biosynthesis
title_short	High δ-aminolevulinic acid uptake in rat cerebral cortex: effect on porphyrin biosynthesis
title_full	High δ-aminolevulinic acid uptake in rat cerebral cortex: effect on porphyrin biosynthesis
title_fullStr	High δ-aminolevulinic acid uptake in rat cerebral cortex: effect on porphyrin biosynthesis
title_full_unstemmed	High δ-aminolevulinic acid uptake in rat cerebral cortex: effect on porphyrin biosynthesis
title_sort	high δ-aminolevulinic acid uptake in rat cerebral cortex: effect on porphyrin biosynthesis
publishDate	1995
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07428413_v111_n1_p143_Juknat http://hdl.handle.net/20.500.12110/paper_07428413_v111_n1_p143_Juknat
work_keys_str_mv	AT juknatadelaana highdaminolevulinicaciduptakeinratcerebralcortexeffectonporphyrinbiosynthesis AT batllealciramariadelcarmen highdaminolevulinicaciduptakeinratcerebralcortexeffectonporphyrinbiosynthesis
_version_	1768545055614697472

High δ-aminolevulinic acid uptake in rat cerebral cortex: effect on porphyrin biosynthesis

Ejemplares similares