Melatonin's antioxidant protection against δ-aminolevulinic acid-induced oxidative damage in rat cerebellum
δ-aminolevulinic acid (ALA) promotes the generation of reactive oxygen species (ROS). Accumulation of ALA, as occurs in acute intermittent porphyria (AIP), is a potential endogenous source of ROS, which can then exert oxidative damage to cell structures. In this work we investigated the role of phar...
Guardado en:
| Publicado: |
1997
|
|---|---|
| Materias: | |
| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07423098_v23_n1_p40_Princ http://hdl.handle.net/20.500.12110/paper_07423098_v23_n1_p40_Princ |
| Aporte de: |
| id |
paper:paper_07423098_v23_n1_p40_Princ |
|---|---|
| record_format |
dspace |
| spelling |
paper:paper_07423098_v23_n1_p40_Princ2023-06-08T15:44:42Z Melatonin's antioxidant protection against δ-aminolevulinic acid-induced oxidative damage in rat cerebellum Melatonin Porphyrinogenesis Rat cerebellum Reactive oxygen species δ-aminolevulinic acid aminolevulinic acid antioxidant malonaldehyde melatonin porphyrin reactive oxygen metabolite animal article cerebellum drug effect lipid peroxidation male metabolism rat Aminolevulinic Acid Animals Antioxidants Cerebellum Lipid Peroxidation Male Malondialdehyde Melatonin Porphyrins Rats Reactive Oxygen Species δ-aminolevulinic acid (ALA) promotes the generation of reactive oxygen species (ROS). Accumulation of ALA, as occurs in acute intermittent porphyria (AIP), is a potential endogenous source of ROS, which can then exert oxidative damage to cell structures. In this work we investigated the role of pharmacological concentrations of melatonin on the deleterious effect of ALA and its effect on porphyrin biosynthesis. Rat cerebellum incubations were carried out with either ALA (1.0 mM) together with increasing concentrations of melatonin (0.1-2.0 mM) or 2.0 mM melatonin together with varying ALA concentrations (0.05-2.0 mM) for different times (1-4 hr). ALA-induced lipid peroxidation was significantly diminished by melatonin in a concentration-dependent manner. In all conditions 2.0 mM melatonin restored malondialdehyde levels to control values. In incubations without ALA, melatonin markedly reduced (36-40%) the basal levels of lipid peroxidation when compared with the corresponding controls. ALA uptake and porphyrin accumulation were increased 30% in incubations with 1.0-2.0 mM ALA for 4 hr in the presence of 2.0 mM melatonin, providing evidence for the involvement of ALA-promoted ROS in the damage of enzymes related to porphyrin biosynthesis. These results are further support for the protective role of melatonin against oxidative damage induced by ALA; this protective action of melatonin is probably due to melatonin's antioxidant and free radical scavenger properties. The development of a new therapeutic approach for AIP patients employing melatonin alone or in combination with conventional treatments should be considered. 1997 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07423098_v23_n1_p40_Princ http://hdl.handle.net/20.500.12110/paper_07423098_v23_n1_p40_Princ |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Melatonin Porphyrinogenesis Rat cerebellum Reactive oxygen species δ-aminolevulinic acid aminolevulinic acid antioxidant malonaldehyde melatonin porphyrin reactive oxygen metabolite animal article cerebellum drug effect lipid peroxidation male metabolism rat Aminolevulinic Acid Animals Antioxidants Cerebellum Lipid Peroxidation Male Malondialdehyde Melatonin Porphyrins Rats Reactive Oxygen Species |
| spellingShingle |
Melatonin Porphyrinogenesis Rat cerebellum Reactive oxygen species δ-aminolevulinic acid aminolevulinic acid antioxidant malonaldehyde melatonin porphyrin reactive oxygen metabolite animal article cerebellum drug effect lipid peroxidation male metabolism rat Aminolevulinic Acid Animals Antioxidants Cerebellum Lipid Peroxidation Male Malondialdehyde Melatonin Porphyrins Rats Reactive Oxygen Species Melatonin's antioxidant protection against δ-aminolevulinic acid-induced oxidative damage in rat cerebellum |
| topic_facet |
Melatonin Porphyrinogenesis Rat cerebellum Reactive oxygen species δ-aminolevulinic acid aminolevulinic acid antioxidant malonaldehyde melatonin porphyrin reactive oxygen metabolite animal article cerebellum drug effect lipid peroxidation male metabolism rat Aminolevulinic Acid Animals Antioxidants Cerebellum Lipid Peroxidation Male Malondialdehyde Melatonin Porphyrins Rats Reactive Oxygen Species |
| description |
δ-aminolevulinic acid (ALA) promotes the generation of reactive oxygen species (ROS). Accumulation of ALA, as occurs in acute intermittent porphyria (AIP), is a potential endogenous source of ROS, which can then exert oxidative damage to cell structures. In this work we investigated the role of pharmacological concentrations of melatonin on the deleterious effect of ALA and its effect on porphyrin biosynthesis. Rat cerebellum incubations were carried out with either ALA (1.0 mM) together with increasing concentrations of melatonin (0.1-2.0 mM) or 2.0 mM melatonin together with varying ALA concentrations (0.05-2.0 mM) for different times (1-4 hr). ALA-induced lipid peroxidation was significantly diminished by melatonin in a concentration-dependent manner. In all conditions 2.0 mM melatonin restored malondialdehyde levels to control values. In incubations without ALA, melatonin markedly reduced (36-40%) the basal levels of lipid peroxidation when compared with the corresponding controls. ALA uptake and porphyrin accumulation were increased 30% in incubations with 1.0-2.0 mM ALA for 4 hr in the presence of 2.0 mM melatonin, providing evidence for the involvement of ALA-promoted ROS in the damage of enzymes related to porphyrin biosynthesis. These results are further support for the protective role of melatonin against oxidative damage induced by ALA; this protective action of melatonin is probably due to melatonin's antioxidant and free radical scavenger properties. The development of a new therapeutic approach for AIP patients employing melatonin alone or in combination with conventional treatments should be considered. |
| title |
Melatonin's antioxidant protection against δ-aminolevulinic acid-induced oxidative damage in rat cerebellum |
| title_short |
Melatonin's antioxidant protection against δ-aminolevulinic acid-induced oxidative damage in rat cerebellum |
| title_full |
Melatonin's antioxidant protection against δ-aminolevulinic acid-induced oxidative damage in rat cerebellum |
| title_fullStr |
Melatonin's antioxidant protection against δ-aminolevulinic acid-induced oxidative damage in rat cerebellum |
| title_full_unstemmed |
Melatonin's antioxidant protection against δ-aminolevulinic acid-induced oxidative damage in rat cerebellum |
| title_sort |
melatonin's antioxidant protection against δ-aminolevulinic acid-induced oxidative damage in rat cerebellum |
| publishDate |
1997 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07423098_v23_n1_p40_Princ http://hdl.handle.net/20.500.12110/paper_07423098_v23_n1_p40_Princ |
| _version_ |
1768541899090558976 |