Male and female reproductive toxicity induced by sub-chronic ethanol exposure in CF-1 mice

Since genetic damage induced by ethanol exposure is controversial and incomplete and because germ and somatic cells constitute bioindicators for monitoring reproductive toxicity and genotoxic actions of ethanol consumption, the purpose of the present investigation was to evaluate morphological sperm...

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Autores principales:	Cebral, Elisa, Abrevaya, Ximena Celeste, Mudry, Marta Dolores
Publicado:	2011
Materias:	CF-1 mice Ethanol Micronucleus Oocyte Spermatozoa alcohol adult animal alcohol blood level alcohol consumption animal cell animal experiment animal model article cell activation cell damage cell nucleus cell structure CF1 mouse conception controlled study cytotoxicity female gamete genotoxicity gestation period male micronucleus mouse mouse strain nonhuman oocyte priority journal reproductive toxicity sperm Alcohol Drinking Animals Female Male Mice Mice, Inbred Strains Micronuclei, Chromosome-Defective Mutagenicity Tests Oocytes Pregnancy Reproduction Mus
Acceso en línea:	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07422091_v27_n4_p237_Cebral http://hdl.handle.net/20.500.12110/paper_07422091_v27_n4_p237_Cebral
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	paper:paper_07422091_v27_n4_p237_Cebral
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spelling	paper:paper_07422091_v27_n4_p237_Cebral2023-06-08T15:44:42Z Male and female reproductive toxicity induced by sub-chronic ethanol exposure in CF-1 mice Cebral, Elisa Abrevaya, Ximena Celeste Mudry, Marta Dolores CF-1 mice Ethanol Micronucleus Oocyte Spermatozoa alcohol adult animal alcohol blood level alcohol consumption animal cell animal experiment animal model article cell activation cell damage cell nucleus cell structure CF1 mouse conception controlled study cytotoxicity female gamete genotoxicity gestation period male micronucleus mouse mouse strain nonhuman oocyte priority journal reproductive toxicity sperm Alcohol Drinking Animals Ethanol Female Male Mice Mice, Inbred Strains Micronuclei, Chromosome-Defective Mutagenicity Tests Oocytes Pregnancy Reproduction Spermatozoa Mus Since genetic damage induced by ethanol exposure is controversial and incomplete and because germ and somatic cells constitute bioindicators for monitoring reproductive toxicity and genotoxic actions of ethanol consumption, the purpose of the present investigation was to evaluate morphological sperm, oocyte alterations and parental genotoxic effects after sub-chronic ethanol intake in the CF-1 outbred mouse strain. Ethanol 10% was administered to CF-1 adult male (treated males, TM) and female (treated females, TF) mice for 27 days, whereas water was given to controls from both sexes too (CM and CF). Post-treatment micronucleus frequency (MN-PCE/1,000/mouse) and gamete morphology were evaluated. To test whether change of female reproductive status results in maternal genotoxicity, CF-1 females received ethanol 10% (exposed group, periconceptionally treated females (PTF)) or water (control group, pregnant control females (PCF)) in drinking water for 17 days previous and up to 10 days of gestation. TM had a high percentage of abnormal spermatozoa vs CM (p < 0.001) and elevated parthenogenetic activated oocyte frequency appeared in TF vs CF (p < 0.001). Sub-chronic ethanol ingestion induced increased MN frequency in TM and TF (p < 0.01). In PTF, where blood alcohol concentrations were between 19-28 mg/dl, very significantly increased MN frequency was found vs PCF (p < 0.01), whereas MN values were similar to TF. These results show that sub-chronic alcohol ingestion in CF-1 mice produces sperm head dysmorphogenesis and oocyte nuclear anomalies, suggesting that morphological abnormalities in germ cells are probably related to parental genotoxicity after ethanol consumption. © 2011 Springer Science+Business Media B.V. Fil:Cebral, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Abrevaya, X.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Mudry, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2011 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07422091_v27_n4_p237_Cebral http://hdl.handle.net/20.500.12110/paper_07422091_v27_n4_p237_Cebral
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	CF-1 mice Ethanol Micronucleus Oocyte Spermatozoa alcohol adult animal alcohol blood level alcohol consumption animal cell animal experiment animal model article cell activation cell damage cell nucleus cell structure CF1 mouse conception controlled study cytotoxicity female gamete genotoxicity gestation period male micronucleus mouse mouse strain nonhuman oocyte priority journal reproductive toxicity sperm Alcohol Drinking Animals Ethanol Female Male Mice Mice, Inbred Strains Micronuclei, Chromosome-Defective Mutagenicity Tests Oocytes Pregnancy Reproduction Spermatozoa Mus
spellingShingle	CF-1 mice Ethanol Micronucleus Oocyte Spermatozoa alcohol adult animal alcohol blood level alcohol consumption animal cell animal experiment animal model article cell activation cell damage cell nucleus cell structure CF1 mouse conception controlled study cytotoxicity female gamete genotoxicity gestation period male micronucleus mouse mouse strain nonhuman oocyte priority journal reproductive toxicity sperm Alcohol Drinking Animals Ethanol Female Male Mice Mice, Inbred Strains Micronuclei, Chromosome-Defective Mutagenicity Tests Oocytes Pregnancy Reproduction Spermatozoa Mus Cebral, Elisa Abrevaya, Ximena Celeste Mudry, Marta Dolores Male and female reproductive toxicity induced by sub-chronic ethanol exposure in CF-1 mice
topic_facet	CF-1 mice Ethanol Micronucleus Oocyte Spermatozoa alcohol adult animal alcohol blood level alcohol consumption animal cell animal experiment animal model article cell activation cell damage cell nucleus cell structure CF1 mouse conception controlled study cytotoxicity female gamete genotoxicity gestation period male micronucleus mouse mouse strain nonhuman oocyte priority journal reproductive toxicity sperm Alcohol Drinking Animals Ethanol Female Male Mice Mice, Inbred Strains Micronuclei, Chromosome-Defective Mutagenicity Tests Oocytes Pregnancy Reproduction Spermatozoa Mus
description	Since genetic damage induced by ethanol exposure is controversial and incomplete and because germ and somatic cells constitute bioindicators for monitoring reproductive toxicity and genotoxic actions of ethanol consumption, the purpose of the present investigation was to evaluate morphological sperm, oocyte alterations and parental genotoxic effects after sub-chronic ethanol intake in the CF-1 outbred mouse strain. Ethanol 10% was administered to CF-1 adult male (treated males, TM) and female (treated females, TF) mice for 27 days, whereas water was given to controls from both sexes too (CM and CF). Post-treatment micronucleus frequency (MN-PCE/1,000/mouse) and gamete morphology were evaluated. To test whether change of female reproductive status results in maternal genotoxicity, CF-1 females received ethanol 10% (exposed group, periconceptionally treated females (PTF)) or water (control group, pregnant control females (PCF)) in drinking water for 17 days previous and up to 10 days of gestation. TM had a high percentage of abnormal spermatozoa vs CM (p < 0.001) and elevated parthenogenetic activated oocyte frequency appeared in TF vs CF (p < 0.001). Sub-chronic ethanol ingestion induced increased MN frequency in TM and TF (p < 0.01). In PTF, where blood alcohol concentrations were between 19-28 mg/dl, very significantly increased MN frequency was found vs PCF (p < 0.01), whereas MN values were similar to TF. These results show that sub-chronic alcohol ingestion in CF-1 mice produces sperm head dysmorphogenesis and oocyte nuclear anomalies, suggesting that morphological abnormalities in germ cells are probably related to parental genotoxicity after ethanol consumption. © 2011 Springer Science+Business Media B.V.
author	Cebral, Elisa Abrevaya, Ximena Celeste Mudry, Marta Dolores
author_facet	Cebral, Elisa Abrevaya, Ximena Celeste Mudry, Marta Dolores
author_sort	Cebral, Elisa
title	Male and female reproductive toxicity induced by sub-chronic ethanol exposure in CF-1 mice
title_short	Male and female reproductive toxicity induced by sub-chronic ethanol exposure in CF-1 mice
title_full	Male and female reproductive toxicity induced by sub-chronic ethanol exposure in CF-1 mice
title_fullStr	Male and female reproductive toxicity induced by sub-chronic ethanol exposure in CF-1 mice
title_full_unstemmed	Male and female reproductive toxicity induced by sub-chronic ethanol exposure in CF-1 mice
title_sort	male and female reproductive toxicity induced by sub-chronic ethanol exposure in cf-1 mice
publishDate	2011
url	https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07422091_v27_n4_p237_Cebral http://hdl.handle.net/20.500.12110/paper_07422091_v27_n4_p237_Cebral
work_keys_str_mv	AT cebralelisa maleandfemalereproductivetoxicityinducedbysubchronicethanolexposureincf1mice AT abrevayaximenaceleste maleandfemalereproductivetoxicityinducedbysubchronicethanolexposureincf1mice AT mudrymartadolores maleandfemalereproductivetoxicityinducedbysubchronicethanolexposureincf1mice
_version_	1768546120169947136

Male and female reproductive toxicity induced by sub-chronic ethanol exposure in CF-1 mice

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