Early effects of insulin-like growth factor-1 in activated human T lymphocytes
This study evaluates the effects of insulin-like growth factor (IGF)-1 receptor (IGF-1R) down-regulation in stimulated T lymphocytes by investigating the expression of early activation proteins CD69, CD25, and interleukin (IL)-2. We found that IGF-1 does not modify CD69 expression but increases tran...
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2001
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| Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07415400_v70_n2_p297_Brocardo http://hdl.handle.net/20.500.12110/paper_07415400_v70_n2_p297_Brocardo |
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paper:paper_07415400_v70_n2_p297_Brocardo2023-06-08T15:44:40Z Early effects of insulin-like growth factor-1 in activated human T lymphocytes CD25 IGF-1 mRNA IGF-1 receptor IL-2 MAPK CD69 antigen interleukin 2 interleukin 2 receptor microtubule associated protein protein kinase (calcium,calmodulin) somatomedin C somatomedin C receptor transcription factor transcription factor ea transcription factor eb unclassified drug antigen expression article autocrine effect controlled study human human cell immunostimulation insulin like activity leukemia cell line microtubule assembly paracrine signaling pathophysiology priority journal protein expression protein modification protein phosphorylation protein synthesis receptor down regulation T lymphocyte activation transcription regulation Antigens, CD Antigens, Differentiation, T-Lymphocyte Down-Regulation Humans Immediate-Early Proteins Insulin-Like Growth Factor I Interleukin-2 Jurkat Cells Kinetics Lymphocyte Activation Mitogen-Activated Protein Kinases Receptor, IGF Type 1 Receptors, Interleukin-2 RNA, Messenger T-Lymphocytes Transcription, Genetic This study evaluates the effects of insulin-like growth factor (IGF)-1 receptor (IGF-1R) down-regulation in stimulated T lymphocytes by investigating the expression of early activation proteins CD69, CD25, and interleukin (IL)-2. We found that IGF-1 does not modify CD69 expression but increases transcription and protein synthesis of CD25 and IL-2. The lowest level of IGF-1R detected after 15 min of activation suggested that the effects of IGF-1 occur at the initiation of cell activation. The activation of IGF-1R was confirmed by IGF-1R phosphorylation and increased phosphorylation of microtubule-associated protein kinase. We also detected the alternative IGF-1 transcripts Ea, with paracrine/autocrine regulation, and Eb, with endocrine regulation, in Jurkat cells and in quiescent T lymphocytes, and we detected IGF-1 protein in the culture medium after stimulation. These data suggest that the proliferative effects of IGF-1 on T lymphocytes include both autocrine/paracrine and endocrine processes. 2001 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07415400_v70_n2_p297_Brocardo http://hdl.handle.net/20.500.12110/paper_07415400_v70_n2_p297_Brocardo |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
CD25 IGF-1 mRNA IGF-1 receptor IL-2 MAPK CD69 antigen interleukin 2 interleukin 2 receptor microtubule associated protein protein kinase (calcium,calmodulin) somatomedin C somatomedin C receptor transcription factor transcription factor ea transcription factor eb unclassified drug antigen expression article autocrine effect controlled study human human cell immunostimulation insulin like activity leukemia cell line microtubule assembly paracrine signaling pathophysiology priority journal protein expression protein modification protein phosphorylation protein synthesis receptor down regulation T lymphocyte activation transcription regulation Antigens, CD Antigens, Differentiation, T-Lymphocyte Down-Regulation Humans Immediate-Early Proteins Insulin-Like Growth Factor I Interleukin-2 Jurkat Cells Kinetics Lymphocyte Activation Mitogen-Activated Protein Kinases Receptor, IGF Type 1 Receptors, Interleukin-2 RNA, Messenger T-Lymphocytes Transcription, Genetic |
| spellingShingle |
CD25 IGF-1 mRNA IGF-1 receptor IL-2 MAPK CD69 antigen interleukin 2 interleukin 2 receptor microtubule associated protein protein kinase (calcium,calmodulin) somatomedin C somatomedin C receptor transcription factor transcription factor ea transcription factor eb unclassified drug antigen expression article autocrine effect controlled study human human cell immunostimulation insulin like activity leukemia cell line microtubule assembly paracrine signaling pathophysiology priority journal protein expression protein modification protein phosphorylation protein synthesis receptor down regulation T lymphocyte activation transcription regulation Antigens, CD Antigens, Differentiation, T-Lymphocyte Down-Regulation Humans Immediate-Early Proteins Insulin-Like Growth Factor I Interleukin-2 Jurkat Cells Kinetics Lymphocyte Activation Mitogen-Activated Protein Kinases Receptor, IGF Type 1 Receptors, Interleukin-2 RNA, Messenger T-Lymphocytes Transcription, Genetic Early effects of insulin-like growth factor-1 in activated human T lymphocytes |
| topic_facet |
CD25 IGF-1 mRNA IGF-1 receptor IL-2 MAPK CD69 antigen interleukin 2 interleukin 2 receptor microtubule associated protein protein kinase (calcium,calmodulin) somatomedin C somatomedin C receptor transcription factor transcription factor ea transcription factor eb unclassified drug antigen expression article autocrine effect controlled study human human cell immunostimulation insulin like activity leukemia cell line microtubule assembly paracrine signaling pathophysiology priority journal protein expression protein modification protein phosphorylation protein synthesis receptor down regulation T lymphocyte activation transcription regulation Antigens, CD Antigens, Differentiation, T-Lymphocyte Down-Regulation Humans Immediate-Early Proteins Insulin-Like Growth Factor I Interleukin-2 Jurkat Cells Kinetics Lymphocyte Activation Mitogen-Activated Protein Kinases Receptor, IGF Type 1 Receptors, Interleukin-2 RNA, Messenger T-Lymphocytes Transcription, Genetic |
| description |
This study evaluates the effects of insulin-like growth factor (IGF)-1 receptor (IGF-1R) down-regulation in stimulated T lymphocytes by investigating the expression of early activation proteins CD69, CD25, and interleukin (IL)-2. We found that IGF-1 does not modify CD69 expression but increases transcription and protein synthesis of CD25 and IL-2. The lowest level of IGF-1R detected after 15 min of activation suggested that the effects of IGF-1 occur at the initiation of cell activation. The activation of IGF-1R was confirmed by IGF-1R phosphorylation and increased phosphorylation of microtubule-associated protein kinase. We also detected the alternative IGF-1 transcripts Ea, with paracrine/autocrine regulation, and Eb, with endocrine regulation, in Jurkat cells and in quiescent T lymphocytes, and we detected IGF-1 protein in the culture medium after stimulation. These data suggest that the proliferative effects of IGF-1 on T lymphocytes include both autocrine/paracrine and endocrine processes. |
| title |
Early effects of insulin-like growth factor-1 in activated human T lymphocytes |
| title_short |
Early effects of insulin-like growth factor-1 in activated human T lymphocytes |
| title_full |
Early effects of insulin-like growth factor-1 in activated human T lymphocytes |
| title_fullStr |
Early effects of insulin-like growth factor-1 in activated human T lymphocytes |
| title_full_unstemmed |
Early effects of insulin-like growth factor-1 in activated human T lymphocytes |
| title_sort |
early effects of insulin-like growth factor-1 in activated human t lymphocytes |
| publishDate |
2001 |
| url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07415400_v70_n2_p297_Brocardo http://hdl.handle.net/20.500.12110/paper_07415400_v70_n2_p297_Brocardo |
| _version_ |
1768544415722242048 |