Biochemical, antimicrobial and molecular characterization of a noncytotoxic bacteriocin produced by Lactobacillus plantarum ST71KS

Lactobacillus (Lb.) plantarum ST71KS was isolated from homemade goat feta cheese and identified using biochemical and molecular biology techniques. As shown by Tricine-SDS-PAGE, this lactic acid bacterium produces a bacteriocin (ST71KS) with an estimated molecular weight of 5.0 kDa. Bacteriocin ST71...

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Detalles Bibliográficos
Autores principales: Wachsman, Mónica Beatriz, Torres, Nicolás I.
Publicado: 2013
Materias:
Antimicrobial activity
Bacteriocin
Bacteriocin-encoding genes
Cytotoxicity
Lactobacillus plantarum
Listeria monocytogenes
bacteriocin
animal
article
cell line
Cercopithecus
cheese
chemistry
genetics
human
isolation and purification
metabolism
microbiology
pH
Vero cell
Animals
Bacteriocins
Cell Line
Cercopithecus aethiops
Cheese
Humans
Hydrogen-Ion Concentration
Vero Cells
Bacteria (microorganisms)
Capra hircus
Lactobacillus
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07400020_v34_n2_p376_Martinez
http://hdl.handle.net/20.500.12110/paper_07400020_v34_n2_p376_Martinez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:Lactobacillus (Lb.) plantarum ST71KS was isolated from homemade goat feta cheese and identified using biochemical and molecular biology techniques. As shown by Tricine-SDS-PAGE, this lactic acid bacterium produces a bacteriocin (ST71KS) with an estimated molecular weight of 5.0 kDa. Bacteriocin ST71KS was not affected by the presence of α-amylase, catalase and remained stable in a wide range of pH and after treatment with Triton X-100, Triton X-114, Tween 20, Tween 80, NaCl, SDS, urea and EDTA. This bacteriocin also remained active after being heated at 100 °C for 2 h and even after 20 min at 121 °C; however, it was inactivated by proteolitic enzymes. Production of bacteriocin ST71KS reached 6400 AU/mL during stationary growth phase of Lb. plantarum cultivated in MRS at 30 °C and 37 °C. Bacteriocin ST71KS displayed a bactericidal effect against Listeria monocytogenes strains 603 and 607 and did not adsorb to the producer cells. Lb. plantarum ST71KS harbors two bacteriocin genes with homology to plantaricin S and pediocin PA-1. These characteristics indicate that bacteriocin ST71KS is a class IIa bacteriocin. The peptide presented no toxic effect when tested in vitro with kidney Vero cells, indicating safe technological application to control L. monocytogenes in foods. © 2013 Elsevier Ltd.

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