Biochemical, antimicrobial and molecular characterization of a noncytotoxic bacteriocin produced by Lactobacillus plantarum ST71KS
Lactobacillus (Lb.) plantarum ST71KS was isolated from homemade goat feta cheese and identified using biochemical and molecular biology techniques. As shown by Tricine-SDS-PAGE, this lactic acid bacterium produces a bacteriocin (ST71KS) with an estimated molecular weight of 5.0 kDa. Bacteriocin ST71...
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Acceso en línea: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07400020_v34_n2_p376_Martinez http://hdl.handle.net/20.500.12110/paper_07400020_v34_n2_p376_Martinez |
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paper:paper_07400020_v34_n2_p376_Martinez2023-06-08T15:44:27Z Biochemical, antimicrobial and molecular characterization of a noncytotoxic bacteriocin produced by Lactobacillus plantarum ST71KS Wachsman, Mónica Beatriz Torres, Nicolás I. Antimicrobial activity Bacteriocin Bacteriocin-encoding genes Cytotoxicity Lactobacillus plantarum Listeria monocytogenes bacteriocin animal article cell line Cercopithecus cheese chemistry genetics human isolation and purification Lactobacillus plantarum metabolism microbiology pH Vero cell Animals Bacteriocins Cell Line Cercopithecus aethiops Cheese Humans Hydrogen-Ion Concentration Lactobacillus plantarum Vero Cells Bacteria (microorganisms) Capra hircus Lactobacillus Lactobacillus plantarum Listeria monocytogenes Lactobacillus (Lb.) plantarum ST71KS was isolated from homemade goat feta cheese and identified using biochemical and molecular biology techniques. As shown by Tricine-SDS-PAGE, this lactic acid bacterium produces a bacteriocin (ST71KS) with an estimated molecular weight of 5.0 kDa. Bacteriocin ST71KS was not affected by the presence of α-amylase, catalase and remained stable in a wide range of pH and after treatment with Triton X-100, Triton X-114, Tween 20, Tween 80, NaCl, SDS, urea and EDTA. This bacteriocin also remained active after being heated at 100 °C for 2 h and even after 20 min at 121 °C; however, it was inactivated by proteolitic enzymes. Production of bacteriocin ST71KS reached 6400 AU/mL during stationary growth phase of Lb. plantarum cultivated in MRS at 30 °C and 37 °C. Bacteriocin ST71KS displayed a bactericidal effect against Listeria monocytogenes strains 603 and 607 and did not adsorb to the producer cells. Lb. plantarum ST71KS harbors two bacteriocin genes with homology to plantaricin S and pediocin PA-1. These characteristics indicate that bacteriocin ST71KS is a class IIa bacteriocin. The peptide presented no toxic effect when tested in vitro with kidney Vero cells, indicating safe technological application to control L. monocytogenes in foods. © 2013 Elsevier Ltd. Fil:Wachsman, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, N.I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 2013 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07400020_v34_n2_p376_Martinez http://hdl.handle.net/20.500.12110/paper_07400020_v34_n2_p376_Martinez |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
Antimicrobial activity Bacteriocin Bacteriocin-encoding genes Cytotoxicity Lactobacillus plantarum Listeria monocytogenes bacteriocin animal article cell line Cercopithecus cheese chemistry genetics human isolation and purification Lactobacillus plantarum metabolism microbiology pH Vero cell Animals Bacteriocins Cell Line Cercopithecus aethiops Cheese Humans Hydrogen-Ion Concentration Lactobacillus plantarum Vero Cells Bacteria (microorganisms) Capra hircus Lactobacillus Lactobacillus plantarum Listeria monocytogenes |
spellingShingle |
Antimicrobial activity Bacteriocin Bacteriocin-encoding genes Cytotoxicity Lactobacillus plantarum Listeria monocytogenes bacteriocin animal article cell line Cercopithecus cheese chemistry genetics human isolation and purification Lactobacillus plantarum metabolism microbiology pH Vero cell Animals Bacteriocins Cell Line Cercopithecus aethiops Cheese Humans Hydrogen-Ion Concentration Lactobacillus plantarum Vero Cells Bacteria (microorganisms) Capra hircus Lactobacillus Lactobacillus plantarum Listeria monocytogenes Wachsman, Mónica Beatriz Torres, Nicolás I. Biochemical, antimicrobial and molecular characterization of a noncytotoxic bacteriocin produced by Lactobacillus plantarum ST71KS |
topic_facet |
Antimicrobial activity Bacteriocin Bacteriocin-encoding genes Cytotoxicity Lactobacillus plantarum Listeria monocytogenes bacteriocin animal article cell line Cercopithecus cheese chemistry genetics human isolation and purification Lactobacillus plantarum metabolism microbiology pH Vero cell Animals Bacteriocins Cell Line Cercopithecus aethiops Cheese Humans Hydrogen-Ion Concentration Lactobacillus plantarum Vero Cells Bacteria (microorganisms) Capra hircus Lactobacillus Lactobacillus plantarum Listeria monocytogenes |
description |
Lactobacillus (Lb.) plantarum ST71KS was isolated from homemade goat feta cheese and identified using biochemical and molecular biology techniques. As shown by Tricine-SDS-PAGE, this lactic acid bacterium produces a bacteriocin (ST71KS) with an estimated molecular weight of 5.0 kDa. Bacteriocin ST71KS was not affected by the presence of α-amylase, catalase and remained stable in a wide range of pH and after treatment with Triton X-100, Triton X-114, Tween 20, Tween 80, NaCl, SDS, urea and EDTA. This bacteriocin also remained active after being heated at 100 °C for 2 h and even after 20 min at 121 °C; however, it was inactivated by proteolitic enzymes. Production of bacteriocin ST71KS reached 6400 AU/mL during stationary growth phase of Lb. plantarum cultivated in MRS at 30 °C and 37 °C. Bacteriocin ST71KS displayed a bactericidal effect against Listeria monocytogenes strains 603 and 607 and did not adsorb to the producer cells. Lb. plantarum ST71KS harbors two bacteriocin genes with homology to plantaricin S and pediocin PA-1. These characteristics indicate that bacteriocin ST71KS is a class IIa bacteriocin. The peptide presented no toxic effect when tested in vitro with kidney Vero cells, indicating safe technological application to control L. monocytogenes in foods. © 2013 Elsevier Ltd. |
author |
Wachsman, Mónica Beatriz Torres, Nicolás I. |
author_facet |
Wachsman, Mónica Beatriz Torres, Nicolás I. |
author_sort |
Wachsman, Mónica Beatriz |
title |
Biochemical, antimicrobial and molecular characterization of a noncytotoxic bacteriocin produced by Lactobacillus plantarum ST71KS |
title_short |
Biochemical, antimicrobial and molecular characterization of a noncytotoxic bacteriocin produced by Lactobacillus plantarum ST71KS |
title_full |
Biochemical, antimicrobial and molecular characterization of a noncytotoxic bacteriocin produced by Lactobacillus plantarum ST71KS |
title_fullStr |
Biochemical, antimicrobial and molecular characterization of a noncytotoxic bacteriocin produced by Lactobacillus plantarum ST71KS |
title_full_unstemmed |
Biochemical, antimicrobial and molecular characterization of a noncytotoxic bacteriocin produced by Lactobacillus plantarum ST71KS |
title_sort |
biochemical, antimicrobial and molecular characterization of a noncytotoxic bacteriocin produced by lactobacillus plantarum st71ks |
publishDate |
2013 |
url |
https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07400020_v34_n2_p376_Martinez http://hdl.handle.net/20.500.12110/paper_07400020_v34_n2_p376_Martinez |
work_keys_str_mv |
AT wachsmanmonicabeatriz biochemicalantimicrobialandmolecularcharacterizationofanoncytotoxicbacteriocinproducedbylactobacillusplantarumst71ks AT torresnicolasi biochemicalantimicrobialandmolecularcharacterizationofanoncytotoxicbacteriocinproducedbylactobacillusplantarumst71ks |
_version_ |
1768543806101127168 |