Synthesis and deposition of PCG‐100, the main pupal cuticle glycoprotein of the medfly Ceratitis capitata

We have studied the developmental expression of the main pupal cuticle glycoprotein, PCG‐100, in the Medfly Ceratitis capitata. A polyclonal antiserum was raised against this protein. Western blotting analysis showed that this glycoprotein is integument‐ and stage‐specific. No PCG‐100 or immunologic...

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Detalles Bibliográficos
Autor principal: Boccaccio, Graciela Lidia
Publicado: 1994
Materias:
Ceratitis capitata
insect epidermis
integument
metamorphosis
Acceso en línea:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07394462_v27_n3_p217_Boccaccio
http://hdl.handle.net/20.500.12110/paper_07394462_v27_n3_p217_Boccaccio
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id paper:paper_07394462_v27_n3_p217_Boccaccio
record_format dspace
spelling paper:paper_07394462_v27_n3_p217_Boccaccio2023-06-08T15:44:24Z Synthesis and deposition of PCG‐100, the main pupal cuticle glycoprotein of the medfly Ceratitis capitata Boccaccio, Graciela Lidia Ceratitis capitata insect epidermis integument metamorphosis We have studied the developmental expression of the main pupal cuticle glycoprotein, PCG‐100, in the Medfly Ceratitis capitata. A polyclonal antiserum was raised against this protein. Western blotting analysis showed that this glycoprotein is integument‐ and stage‐specific. No PCG‐100 or immunologically related polypeptides were detected in other tissues or instars. As studied by microinjection of [35S]methionine in individual flies, in vivo synthesis and deposition of PCG‐100 begins approximately 48 h after the onset of pupariation, shortly after the time of head eversion. Synthesis is maximal at 54–64 h, decreases at 72 h, and practically ceases in fully shaped 4‐day‐old pupae. The time required for PCG‐100 deposition into the cuticle was found to be less than 10 min after its synthesis. This is the first time such in vivo analysis has been performed. © 1994 Wiley‐Liss, Inc. Copyright © 1994 Wiley‐Liss, Inc. Fil:Boccaccio, G.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. 1994 https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07394462_v27_n3_p217_Boccaccio http://hdl.handle.net/20.500.12110/paper_07394462_v27_n3_p217_Boccaccio
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Ceratitis capitata
insect epidermis
integument
metamorphosis
spellingShingle Ceratitis capitata
insect epidermis
integument
metamorphosis
Boccaccio, Graciela Lidia
Synthesis and deposition of PCG‐100, the main pupal cuticle glycoprotein of the medfly Ceratitis capitata
topic_facet Ceratitis capitata
insect epidermis
integument
metamorphosis
description We have studied the developmental expression of the main pupal cuticle glycoprotein, PCG‐100, in the Medfly Ceratitis capitata. A polyclonal antiserum was raised against this protein. Western blotting analysis showed that this glycoprotein is integument‐ and stage‐specific. No PCG‐100 or immunologically related polypeptides were detected in other tissues or instars. As studied by microinjection of [35S]methionine in individual flies, in vivo synthesis and deposition of PCG‐100 begins approximately 48 h after the onset of pupariation, shortly after the time of head eversion. Synthesis is maximal at 54–64 h, decreases at 72 h, and practically ceases in fully shaped 4‐day‐old pupae. The time required for PCG‐100 deposition into the cuticle was found to be less than 10 min after its synthesis. This is the first time such in vivo analysis has been performed. © 1994 Wiley‐Liss, Inc. Copyright © 1994 Wiley‐Liss, Inc.
author Boccaccio, Graciela Lidia
author_facet Boccaccio, Graciela Lidia
author_sort Boccaccio, Graciela Lidia
title Synthesis and deposition of PCG‐100, the main pupal cuticle glycoprotein of the medfly Ceratitis capitata
title_short Synthesis and deposition of PCG‐100, the main pupal cuticle glycoprotein of the medfly Ceratitis capitata
title_full Synthesis and deposition of PCG‐100, the main pupal cuticle glycoprotein of the medfly Ceratitis capitata
title_fullStr Synthesis and deposition of PCG‐100, the main pupal cuticle glycoprotein of the medfly Ceratitis capitata
title_full_unstemmed Synthesis and deposition of PCG‐100, the main pupal cuticle glycoprotein of the medfly Ceratitis capitata
title_sort synthesis and deposition of pcg‐100, the main pupal cuticle glycoprotein of the medfly ceratitis capitata
publishDate 1994
url https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_07394462_v27_n3_p217_Boccaccio
http://hdl.handle.net/20.500.12110/paper_07394462_v27_n3_p217_Boccaccio
work_keys_str_mv AT boccacciogracielalidia synthesisanddepositionofpcg100themainpupalcuticleglycoproteinofthemedflyceratitiscapitata
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